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Biosensors 2018, 8(4), 118; https://doi.org/10.3390/bios8040118

Microwave Spectroscopic Detection of Human Hsp70 Protein on Annealed Gold Nanostructures on ITO Glass Strips

1
Light, Nanomaterials and Nanotechnology (L2N), FRE-CNRS 2019, Institute Charles Delaunay (ICD), University of Technology of Troyes, 12 Rue Marie Curie CS 42060, 10004 Troyes CEDEX, France
2
Laboratory Interdisciplinaire Carnot de Bourgogne (ICB), UMR-CNRS 6303, University of Bourgogne Franche-Comté, 9 Avenue Alain Savary, 21078 Dijon CEDEX, France
*
Author to whom correspondence should be addressed.
Received: 10 October 2018 / Revised: 10 November 2018 / Accepted: 21 November 2018 / Published: 27 November 2018
(This article belongs to the Special Issue Immunoanalytical Tool with Electrochemical and Optical Detection)
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Abstract

Conductive indium-tin oxide (ITO) and non-conductive glass substrates were successfully modified with embedded gold nanoparticles (AuNPs) formed by controlled thermal annealing at 550 °C for 8 h in a preselected oven. The authors characterized the formation of AuNPs using two microscopic techniques: scanning electron microscopy (SEM) and atomic force microscopy (AFM). The analytical performances of the nanostructured-glasses were compared regarding biosensing of Hsp70, an ATP-driven molecular chaperone. In this work, the human heat-shock protein (Hsp70), was chosen as a model biomarker of body stress disorders for microwave spectroscopic investigations. It was found that microwave screening at 4 GHz allowed for the first time the detection of 12 ng/µL/cm2 of Hsp70. View Full-Text
Keywords: biosensor; ITO functionalization; label-free microwave detection; molecular chaperone Hsp70 biosensor; ITO functionalization; label-free microwave detection; molecular chaperone Hsp70
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Ionescu, R.E.; Selon, R.; Pocholle, N.; Zhou, L.; Rumyantseva, A.; Bourillot, E.; Lesniewska, E. Microwave Spectroscopic Detection of Human Hsp70 Protein on Annealed Gold Nanostructures on ITO Glass Strips. Biosensors 2018, 8, 118.

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