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Biosensors 2018, 8(4), 117; https://doi.org/10.3390/bios8040117

A Liposomal Platform for Sensing of Extracellular Analytes Near Cells

1
ARC Centre of Excellence for Nanoscale BioPhotonics, Institute of Photonics and Advanced Sensing, Department of Chemistry, School of Physical Sciences, The University of Adelaide, Adelaide SA 5005, Australia
2
Discipline of Physiology, Faculty of Health Sciences, The University of Adelaide, Adelaide SA 5005, Australia
3
Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide SA 5005, Australia
4
Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne VIC 3010, Australia
*
Authors to whom correspondence should be addressed.
Received: 10 October 2018 / Revised: 20 November 2018 / Accepted: 21 November 2018 / Published: 26 November 2018
Full-Text   |   PDF [2358 KB, uploaded 26 November 2018]   |  

Abstract

Cell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been incorporated into yeast total lipid extract-based liposomes to suppress inherent cell permeability to allow the detection of extracellular Ca2+, GSH, and Zn2+, respectively. The repurposed sensors have enhanced aqueous solubility and the ability to quantitatively measure biologically relevant concentrations of Ca2+ (0.25 mM–1 mM), Zn2+ (6.25 µM–50 µM), and GSH (0.25 mM–1 mM) by fluorescence in aqueous media. In addition, the liposomal sensors are nontoxic to HEK293 cells and have the ability to detect exogenously added Zn2+ (1 mM), Ca2+ (1 mM), or GSH (1 mM) near cells without internalisation. This new sensing platform provides a means to repurpose a range of intracellular fluorescent sensors to specifically detect extracellular analytes, while also improving biocompatibility for overall enhanced use in a wide range of biomedical applications. View Full-Text
Keywords: liposome; biosensing; extracellular sensing; fluorescence; small-molecule sensors liposome; biosensing; extracellular sensing; fluorescence; small-molecule sensors
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Zhang, X.; Heng, S.; Pei, J.; Morey, J.R.; McDevitt, C.A.; Abell, A.D. A Liposomal Platform for Sensing of Extracellular Analytes Near Cells. Biosensors 2018, 8, 117.

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