A Liposomal Platform for Sensing of Extracellular Analytes Near Cells
AbstractCell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been incorporated into yeast total lipid extract-based liposomes to suppress inherent cell permeability to allow the detection of extracellular Ca2+, GSH, and Zn2+, respectively. The repurposed sensors have enhanced aqueous solubility and the ability to quantitatively measure biologically relevant concentrations of Ca2+ (0.25 mM–1 mM), Zn2+ (6.25 µM–50 µM), and GSH (0.25 mM–1 mM) by fluorescence in aqueous media. In addition, the liposomal sensors are nontoxic to HEK293 cells and have the ability to detect exogenously added Zn2+ (1 mM), Ca2+ (1 mM), or GSH (1 mM) near cells without internalisation. This new sensing platform provides a means to repurpose a range of intracellular fluorescent sensors to specifically detect extracellular analytes, while also improving biocompatibility for overall enhanced use in a wide range of biomedical applications. View Full-Text
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Zhang, X.; Heng, S.; Pei, J.; Morey, J.R.; McDevitt, C.A.; Abell, A.D. A Liposomal Platform for Sensing of Extracellular Analytes Near Cells. Biosensors 2018, 8, 117.
Zhang X, Heng S, Pei J, Morey JR, McDevitt CA, Abell AD. A Liposomal Platform for Sensing of Extracellular Analytes Near Cells. Biosensors. 2018; 8(4):117.Chicago/Turabian Style
Zhang, Xiaozhou; Heng, Sabrina; Pei, Jinxin; Morey, Jacqueline R.; McDevitt, Christopher A.; Abell, Andrew D. 2018. "A Liposomal Platform for Sensing of Extracellular Analytes Near Cells." Biosensors 8, no. 4: 117.
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