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Multiplex Lateral Flow Immunoassay: An Overview of Strategies towards High-throughput Point-of-Need Testing

Department of Chemistry, University of Turin, Via Giuria, 5, I-10125 Turin, Italy
Author to whom correspondence should be addressed.
Biosensors 2019, 9(1), 2;
Received: 15 November 2018 / Revised: 19 December 2018 / Accepted: 23 December 2018 / Published: 26 December 2018
(This article belongs to the Special Issue Immunoanalytical Tool with Electrochemical and Optical Detection)
PDF [1535 KB, uploaded 26 December 2018]


Simultaneous measurement of different substances from a single sample is an emerging issue for achieving efficient and high-throughput detection in several fields of application. Although immunoanalytical techniques have well-established and prevailing advantages over alternative screening analytical platforms, one of the incoming challenges for immunoassay is exact multiplexing. Lateral flow immunoassay (LFIA) is a leading immunoanalytical technique for onsite analysis, thanks to its simplicity, rapidity, and cost-effectiveness. Moreover, LFIA architecture is adaptable to multiplexing, and is therefore a possible answer to the pressing demand of multiplexing point-of-need analysis. This review presents an overview of diverse approaches for multiplex LFIA, with a special focus on strategies based on new types of magnetic, fluorescent, and colored labels. View Full-Text
Keywords: immunoassay; rapid test; point-of-care testing; immunochromatographic test immunoassay; rapid test; point-of-care testing; immunochromatographic test

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Anfossi, L.; Di Nardo, F.; Cavalera, S.; Giovannoli, C.; Baggiani, C. Multiplex Lateral Flow Immunoassay: An Overview of Strategies towards High-throughput Point-of-Need Testing. Biosensors 2019, 9, 2.

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