Next Article in Journal
Continuous Glucose Monitoring in Resource-Constrained Settings for Hypoglycaemia Detection: Looking at the Problem from the Other Side of the Coin
Next Article in Special Issue
Fluorescent Nanosensor Based on Molecularly Imprinted Polymers Coated on Graphene Quantum Dots for Fast Detection of Antibiotics
Previous Article in Journal
Olfactory Dysfunction as a Global Biomarker for Sniffing out Alzheimer’s Disease: A Meta-Analysis
Article Menu
Issue 2 (June) cover image

Export Article

Open AccessArticle
Biosensors 2018, 8(2), 42; https://doi.org/10.3390/bios8020042

Laser Scribed Graphene Biosensor for Detection of Biogenic Amines in Food Samples Using Locally Sourced Materials

1
Department of Food Engineering, Universidad del Valle, Cali 760032, Colombia
2
Department of Biological and Agricultural Engineering, Texas A&M University, College Station, TX 77843, USA
3
Department of Biology, Universidad del Valle, Cali 760032, Colombia
4
Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA
5
Department of Agricultural and Biological Engineering, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA
*
Author to whom correspondence should be addressed.
Received: 6 February 2018 / Revised: 11 April 2018 / Accepted: 19 April 2018 / Published: 24 April 2018
(This article belongs to the Special Issue Food Safety and Quality Monitoring)
Full-Text   |   PDF [4073 KB, uploaded 3 May 2018]   |  

Abstract

In foods, high levels of biogenic amines (BA) are the result of microbial metabolism that could be affected by temperatures and storage conditions. Thus, the level of BA is commonly used as an indicator of food safety and quality. This manuscript outlines the development of laser scribed graphene electrodes, with locally sourced materials, for reagent-free food safety biosensing. To fabricate the biosensors, the graphene surface was functionalized with copper microparticles and diamine oxidase, purchased from a local supermarket; and then compared to biosensors fabricated with analytical grade materials. The amperometric biosensor exhibits good electrochemical performance, with an average histamine sensitivity of 23.3 µA/mM, a lower detection limit of 11.6 µM, and a response time of 7.3 s, showing similar performance to biosensors constructed from analytical grade materials. We demonstrated the application of the biosensor by testing total BA concentration in fish paste samples subjected to fermentation with lactic acid bacteria. Biogenic amines concentrations prior to lactic acid fermentation were below the detection limit of the biosensor, while concentration after fermentation was 19.24 ± 8.21 mg histamine/kg, confirming that the sensor was selective in a complex food matrix. The low-cost, rapid, and accurate device is a promising tool for biogenic amine estimation in food samples, particularly in situations where standard laboratory techniques are unavailable, or are cost prohibitive. This biosensor can be used for screening food samples, potentially limiting food waste, while reducing chances of foodborne outbreaks. View Full-Text
Keywords: biogenic amines; laser scribed graphene; diamine oxidase; disposable sensor; food quality; risk assessment; planetary health biogenic amines; laser scribed graphene; diamine oxidase; disposable sensor; food quality; risk assessment; planetary health
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Vanegas, D.C.; Patiño, L.; Mendez, C.; Oliveira, D.A.; Torres, A.M.; Gomes, C.L.; McLamore, E.S. Laser Scribed Graphene Biosensor for Detection of Biogenic Amines in Food Samples Using Locally Sourced Materials. Biosensors 2018, 8, 42.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top