Functionalized Asymmetric Bola-Type Amphiphiles for Efficient Gene and Drug Delivery
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials and Methods
2.2. Synthesis of the Bolaamphiphiles
2.2.1. Preparation of Compound 2
2.2.2. Preparation of Compound 3
2.2.3. Preparation of the Target Bolaamphiphiles
2.3. Formation of Bolasomes and Bolaplexes
2.4. Gel Retardation Assay
2.5. In Vitro Transfection Procedure
2.6. Cytotoxicity Assays
2.7. Flow Cytometry Assay
2.8. Confocal Laser Scanning Microscopy (CLSM)
2.9. Determination of the Critical Aggregation Concentration (CAC)
2.10. Loading of DOX into Bolaamphiphiles Aggregates
3. Results and Discussion
3.1. Synthesis of the Bolaamphiphiles
3.2. Interaction between the Bolasomes and DNA
3.3. In Vitro Gene Transfection Studies
3.4. Cytotoxicity
3.5. In Vitro Drug Delivery Studies
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Material | NP Preparation | CAC [mg/mL] | Drug Loading | |||
---|---|---|---|---|---|---|
Size [nm] | ζ-Potential [mV] | PDI | EE [%] | DL [%] | ||
Lys-His | 119.9 ± 3.1 | 37.7 ± 4.0 | 0.311 ± 0.072 | 0.022 | - | - |
Lys-TPP | 122.0 ± 2.2 | 34.5 ± 1.7 | 0.258 ± 0.017 | 0.015 | - | - |
Lys-Gal | 91.4 ± 5.3 | 28.7 ± 1.6 | 0.357 ± 0.038 | 0.021 | - | - |
Lys-Biotin | 105.0 ± 1.4 | 33.5 ± 1.3 | 0.316 ± 0.020 | 0.031 | - | - |
Lys-14-10 | 109.7 ± 0.4 | 42.2 ± 4.3 | 0.214 ± 0.020 | 0.061 | - | - |
DOX@Lys-His | 149.2 ± 1.7 | 36.6 ± 1.6 | 0.219 ± 0.003 | - | 97.5 ± 0.5 | 16.3 ± 0.07 |
DOX@Lys-TPP | 158.0 ± 2.4 | 32.8 ± 0.9 | 0.119 ± 0.017 | - | 76.3 ± 0.3 | 13.2 ± 0.04 |
DOX@Lys-Gal | 131.7 ± 3.1 | 34.9 ± 0.6 | 0.318 ± 0.026 | - | 70.5 ± 0.5 | 12.4 ± 0.08 |
DOX@Lys-Biotin | 123.1 ± 0.4 | 41.3 ± 1.1 | 0.211 ± 0.012 | - | 86.5 ± 0.5 | 14.7 ± 0.07 |
DOX@Lys-14-10 | 198.1 ± 2.5 | 39.0 ± 0.6 | 0.154 ± 0.007 | - | 81.0 ± 0.5 | 13.9 ± 0.07 |
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Huang, Z.; Zhao, D.-M.; Deng, X.; Zhang, J.; Zhang, Y.-M.; Yu, X.-Q. Functionalized Asymmetric Bola-Type Amphiphiles for Efficient Gene and Drug Delivery. Nanomaterials 2018, 8, 115. https://doi.org/10.3390/nano8020115
Huang Z, Zhao D-M, Deng X, Zhang J, Zhang Y-M, Yu X-Q. Functionalized Asymmetric Bola-Type Amphiphiles for Efficient Gene and Drug Delivery. Nanomaterials. 2018; 8(2):115. https://doi.org/10.3390/nano8020115
Chicago/Turabian StyleHuang, Zheng, Dong-Mei Zhao, Xuan Deng, Ji Zhang, Yi-Mei Zhang, and Xiao-Qi Yu. 2018. "Functionalized Asymmetric Bola-Type Amphiphiles for Efficient Gene and Drug Delivery" Nanomaterials 8, no. 2: 115. https://doi.org/10.3390/nano8020115