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Article

Green Synthesized Magnetic Nanoparticles as Effective Nanosupport for the Immobilization of Lipase: Application for the Synthesis of Lipophenols

1
Biotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, Greece
2
Department of Materials Science and Engineering, Cornell University, Ithaca, NY 14853, USA
3
Department of Materials Science and Engineering, University of Ioannina, 45110 Ioannina, Greece
*
Author to whom correspondence should be addressed.
Academic Editors: Angelina Angelova and José Luis Barriada Pereira
Nanomaterials 2021, 11(2), 458; https://doi.org/10.3390/nano11020458
Received: 19 December 2020 / Revised: 3 February 2021 / Accepted: 8 February 2021 / Published: 11 February 2021
(This article belongs to the Special Issue Green Synthesis of Nanomaterials and Its Applications)
In this work, hybrid zinc oxide–iron oxide (ZnOFe) magnetic nanoparticles were synthesized employing Olea europaea leaf aqueous extract as a reducing/chelating and capping medium. The resulting magnetic nanoparticles were characterized by basic spectroscopic and microscopic techniques, namely, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), fourier-transform infrared (FTIR) and atomic force microscopy (AFM), exhibiting a spherical shape, average size of 15–17 nm, and a functionalized surface. Lipase from Thermomyces lanuginosus (TLL) was efficiently immobilized on the surface of ZnOFe nanoparticles through physical absorption. The activity of immobilized lipase was found to directly depend on the enzyme to support the mass ratio, and also demonstrated improved pH and temperature activity range compared to free lipase. Furthermore, the novel magnetic nanobiocatalyst (ZnOFe-TLL) was applied to the preparation of hydroxytyrosyl fatty acid esters, including derivatives with omega-3 fatty acids, in non-aqueous media. Conversion yields up to 90% were observed in non-polar solvents, including hydrophobic ionic liquids. Different factors affecting the biocatalyst performance were studied. ZnOFe-TLL was reutilized for eight subsequent cycles, exhibiting 90% remaining esterification activity (720 h of total operation at 50 °C). The green synthesized magnetic nanoparticles, reported here for the first time, are excellent candidates as nanosupports for the immobilization of enzymes with industrial interest, giving rise to nanobiocatalysts with elevated features. View Full-Text
Keywords: green synthesis; Olea europaea; magnetic nanoparticles; lipase; immobilization; biocatalysis; bioactive lipophenols; hydroxytyrosol green synthesis; Olea europaea; magnetic nanoparticles; lipase; immobilization; biocatalysis; bioactive lipophenols; hydroxytyrosol
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MDPI and ACS Style

Fotiadou, R.; Chatzikonstantinou, A.V.; Hammami, M.A.; Chalmpes, N.; Moschovas, D.; Spyrou, K.; Polydera, A.C.; Avgeropoulos, A.; Gournis, D.; Stamatis, H. Green Synthesized Magnetic Nanoparticles as Effective Nanosupport for the Immobilization of Lipase: Application for the Synthesis of Lipophenols. Nanomaterials 2021, 11, 458. https://doi.org/10.3390/nano11020458

AMA Style

Fotiadou R, Chatzikonstantinou AV, Hammami MA, Chalmpes N, Moschovas D, Spyrou K, Polydera AC, Avgeropoulos A, Gournis D, Stamatis H. Green Synthesized Magnetic Nanoparticles as Effective Nanosupport for the Immobilization of Lipase: Application for the Synthesis of Lipophenols. Nanomaterials. 2021; 11(2):458. https://doi.org/10.3390/nano11020458

Chicago/Turabian Style

Fotiadou, Renia, Alexandra V. Chatzikonstantinou, Mohamed A. Hammami, Nikolaos Chalmpes, Dimitrios Moschovas, Konstantinos Spyrou, Angeliki C. Polydera, Apostolos Avgeropoulos, Dimitrios Gournis, and Haralambos Stamatis. 2021. "Green Synthesized Magnetic Nanoparticles as Effective Nanosupport for the Immobilization of Lipase: Application for the Synthesis of Lipophenols" Nanomaterials 11, no. 2: 458. https://doi.org/10.3390/nano11020458

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