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Article

The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

1
Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech Republic
2
Institute of Macromolecular Chemistry of the Czech Academy of Sciences, Heyrovskeho nam. 2, 162 06 Prague 6, Czech Republic
3
Faculty of Textile Engineering, Technical University of Liberec, Studentska 1402/2, 461 17 Liberec 1, Czech Republic
4
Faculty of Science, Humanities and Education, Technical University of Liberec, Studentska 1402/2, 461 17 Liberec 1, Czech Republic
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Institute of Nanomaterials, Advanced Technologies and Innovation, Bendlova 1409/7, 460 01 Liberec 1, Czech Republic
6
Faculty of Health, Technical University of Liberec, Studentska 1402/2, 461 17 Liberec 1, Czech Republic
7
Regional Hospital Liberec, Husova 357/28, 460 01 Liberec 1, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Amir Fahmi
Nanomaterials 2021, 11(2), 457; https://doi.org/10.3390/nano11020457
Received: 8 January 2021 / Revised: 2 February 2021 / Accepted: 8 February 2021 / Published: 11 February 2021
(This article belongs to the Special Issue Hybrid Nanofibers: Fabrication, Properties and Applications)
Chronic wounds affect millions of patients worldwide, and it is estimated that this number will increase steadily in the future due to population ageing. The research of new therapeutic approaches to wound healing includes the development of nanofibrous meshes and the use of platelet lysate (PL) to stimulate skin regeneration. This study considers a combination of a degradable electrospun nanofibrous blend of poly(L-lactide-co-ε-caprolactone) and poly(ε-caprolactone) (PLCL/PCL) membranes (NF) and fibrin loaded with various concentrations of PL aimed at the development of bioactive skin wound healing dressings. The cytocompatibility of the NF membranes, as well as the effect of PL, was evaluated in both monocultures and co-cultures of human keratinocytes and human endothelial cells. We determined that the keratinocytes were able to adhere on all the membranes, and their increased proliferation and differentiation was observed on the membranes that contained fibrin with at least 50% of PL (Fbg + PL) after 14 days. With respect to the co-culture experiments, the membranes with fibrin with 20% of PL were observed to enhance the metabolic activity of endothelial cells and their migration, and the proliferation and differentiation of keratinocytes. The results suggest that the newly developed NF combined with fibrin and PL, described in the study, provides a promising dressing for chronic wound healing purposes. View Full-Text
Keywords: electrospun nanofibre; skin wound healing; keratinocytes; platelet lysate; fibrin; endothelial cells; in vitro co-culture system electrospun nanofibre; skin wound healing; keratinocytes; platelet lysate; fibrin; endothelial cells; in vitro co-culture system
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MDPI and ACS Style

Blanquer, A.; Musilkova, J.; Filova, E.; Taborska, J.; Brynda, E.; Riedel, T.; Klapstova, A.; Jencova, V.; Mullerova, J.; Kostakova, E.K.; Prochazkova, R.; Bacakova, L. The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System. Nanomaterials 2021, 11, 457. https://doi.org/10.3390/nano11020457

AMA Style

Blanquer A, Musilkova J, Filova E, Taborska J, Brynda E, Riedel T, Klapstova A, Jencova V, Mullerova J, Kostakova EK, Prochazkova R, Bacakova L. The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System. Nanomaterials. 2021; 11(2):457. https://doi.org/10.3390/nano11020457

Chicago/Turabian Style

Blanquer, Andreu, Jana Musilkova, Elena Filova, Johanka Taborska, Eduard Brynda, Tomas Riedel, Andrea Klapstova, Vera Jencova, Jana Mullerova, Eva K. Kostakova, Renata Prochazkova, and Lucie Bacakova. 2021. "The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System" Nanomaterials 11, no. 2: 457. https://doi.org/10.3390/nano11020457

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