Effect of Different Immersion Tank Water Temperatures on the Microbiological Quality of Rabbit Carcasses
Abstract
:1. Introduction
2. Materials and Methods
2.1. Characteristics of the Establishment and Samples
2.2. Count of Indicator Microorganisms
2.3. Salmonella spp. Isolation
2.4. Statistical Analysis
3. Results
3.1. Count of Indicator Microorganisms on the Surface of the Rabbit Carcass
3.2. Count of Indicator Microorganisms in Pre-Chiller Water and Ice
3.3. Presence of Salmonella spp.
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
- Petracci, M.; Soglia, F.; Leroy, F. Rabbit Meat in Need of a Hat-Trick: From Tradition to Innovation (and Back). Meat Sci. 2018, 146, 93–100. [Google Scholar] [CrossRef] [PubMed]
- Tufarelli, V.; Tateo, A.; Schiavitto, M.; Mazzei, D.; Calzaretti, G.; Laudadio, V. Evaluating Productive Performance, Meat Quality and Oxidation Products of Italian White Breed Rabbits Under Free-Range and Cage Rearing System. Anim. Biosci. 2022, 35, 884–891. [Google Scholar] [CrossRef] [PubMed]
- Cullere, M.; Dalle Zotte, A. Rabbit Meat Production and Consumption: State of Knowledge and Future Perspectives. Meat Sci. 2018, 143, 137–146. [Google Scholar] [CrossRef]
- de Cerqueira Magalhães, L.C.; Costa, R.B.; de Camargo, G.M.F. Consumption of Rabbit Meat in Brazil: Potential and Limitations. Meat Sci. 2022, 191, 108873. [Google Scholar] [CrossRef] [PubMed]
- Abdel-Naeem, H.H.S.; Sallam, K.I.; Zaki, H.M.B.A. Effect of Different Cooking Methods of Rabbit Meat on Topographical Changes, Physicochemical Characteristics, Fatty Acids Profile, Microbial Quality and Sensory Attributes. Meat Sci. 2021, 181, 108612. [Google Scholar] [CrossRef] [PubMed]
- Bozzo, G.; Dimuccio, M.; Casalino, G.; Ceci, E.; D’Amico, F.; Petrontino, A.; Bonerba, E.; Camarda, A.; Circella, E. Preliminary Evidene Regarding the Detection of Cortisol and IL-6 to Asses Animal Welfare in Various Rabbit Housing Systems. Agriculture 2022, 12, 1622. [Google Scholar] [CrossRef]
- FAO Representante Da FAO Brasil Apresenta Cenário Da Demanda Por Alimentos. Available online: https://www.fao.org/brasil/noticias/detail-events/fr/c/901168/ (accessed on 6 December 2022).
- BRASIL Regulamentos Comuns Aos Produtos Cárneos e Seus Derivados. Available online: https://www.gov.br/agricultura/pt-br/assuntos/suasa/regulamentos-tecnicos-de-identidade-e-qualidade-de-produtos-de-origem-animal-1/rtiq-carneos-e-seus-derivados-1 (accessed on 6 December 2022).
- de M. Franco, B.D.G.; Landgraf, M. Microbiologia Dos Alimentos, 1st ed.; Editora Atheneu: São Paulo, Brazil, 2008. [Google Scholar]
- Perez-Arnedo, I.; Cantalejo, M.J.; Martínez-Laorden, A.; Gonzalez-Fandos, E. Effect of Processing on the Microbiological Quality and Safety of Chicken Carcasses at Slaughterhouse. Int. J. Food Sci. Technol. 2021, 56, 1855–1864. [Google Scholar] [CrossRef]
- Souza, L.C.T.; Pereira, J.G.; Spina, T.L.B.; Izidoro, T.B.; Oliveira, A.C.; Pinto, J.P.A.N. Microbiological Evaluation of Chicken Carcasses in an Immersion Chilling System with Water Renewal at 8 and 16 Hours. J. Food Prot. 2012, 75, 973–975. [Google Scholar] [CrossRef]
- Ferreira, A.; Kunh, S.S.; Cremonez, P.A.; Dieter, J.; Teleken, J.G.; Sampaio, S.C.; Kunh, P.D. Brazilian Poultry Activity Waste: Destinations and Energetic Potential. Renew. Sustain. Energy Rev. 2018, 81, 3081–3089. [Google Scholar] [CrossRef]
- Petracci, M.; Cavani, C. Rabbit Meat Processing: Historical Perspective to Future Directions. World Rabbit Sci. 2013, 21, 217–226. [Google Scholar] [CrossRef]
- Lopes, M.; Galhardo, J.A.; de Oliveira, J.T.; Tamanini, R.; Sanches, S.F.; Muller, E.E. Ernst Eckehardt Muller Pesquisa de Salmonella spp. e Microrganismos Indicadores Em Carcaçasde Frango e Água de Tanques de Pré-Resfriamento Em Abatedouro de Aves. Semin. CIENCIAS Agrar. 2007, 28, 465–475. [Google Scholar] [CrossRef] [Green Version]
- Silva, N.; Junqueira, V.C.A.; de A. Silveira, N.F.; Taniwaki, M.H.; Gomes, R.A.R.; Okazaki, M.M.; Iamanaka, B.T.; Katayama, M. Manual de Métodos de Análise Microbiológica de Alimentos e Água, 5th ed.; Blucher: São Paulo, Brazil, 2017. [Google Scholar]
- Cryan, J.F.; O’Riordan, K.J.; Cowan, C.S.M.; Sandhu, K.V.; Bastiaanssen, T.F.S.; Boehme, M.; Codagnone, M.G.; Cussotto, S.; Fulling, C.; Golubeva, A.V.; et al. The Microbiota-Gut-Brain Axis. Physiol. Rev. 2019, 99, 1877–2013. [Google Scholar] [CrossRef] [PubMed]
- Lillard, H.S. The Impact of Commercial Processing Procedures on the Bacterial Contamination and Cross-Contamination of Broiler Carcasses. J. Food Prot. 1990, 53, 202–204. [Google Scholar] [CrossRef] [PubMed]
- Castrica, M.; Menchetti, L.; Balzaretti, C.M.; Branciari, R.; Ranucci, D.; Cotozzolo, E.; Vigo, D.; Curone, G.; Brecchia, G.; Miraglia, D. Impact of Dietary Supplementation with Goji Berries (Lycium barbarum) on Microbiological Quality, Physico-Chemical, and Sensory Characteristics of Rabbit Meat. Foods 2020, 9, 1480. [Google Scholar] [CrossRef]
- Simas, V.S.; dos Santos, F.F.; Gouvêa, R.; de Aquino, M.H.C.; da Costa Abreu, D.L.; do Nascimento, E.R.; de Almeida Pereira, V.L. Immersion Chilling in Fecal Coliforms Count Reduction on Broiler Carcasses. Ciência Rural 2013, 43, 1618–1622. [Google Scholar] [CrossRef] [Green Version]
- Maharjan, S.; Rayamajhee, B.; Chhetri, V.S.; Sherchan, S.P.; Panta, O.P.; Karki, T.B. Microbial Quality of Poultry Meat in an ISO 22000:2005 Certified Poultry Processing Plant of Kathmandu Valley. Int. J. Food Contam. 2019, 6, 8. [Google Scholar] [CrossRef] [Green Version]
- Bortolaia, V.; Espinosa-Gongora, C.; Guardabassi, L. Human Health Risks Associated with Antimicrobial-Resistant Enterococci and Staphylococcus aureus on Poultry Meat. Clin. Microbiol. Infect. 2016, 22, 130–140. [Google Scholar] [CrossRef] [Green Version]
- Kohler, R.; Krause, G.; Beutin, L.; Stephan, R.; Zweifel, C. Shedding of Food-Borne Pathogens and Microbiological Carcass Contamination in Rabbits at Slaughter. Vet. Microbiol. 2008, 132, 149–157. [Google Scholar] [CrossRef] [Green Version]
- Merz, A.; Stephan, R.; Johler, S. Genotyping and DNA Microarray Based Characterization of Staphylococcus aureus Isolates from Rabbit Carcasses. Meat Sci. 2016, 112, 86–89. [Google Scholar] [CrossRef]
- Hermans, K.; Haesebrouck, F.; Vaneechoutte, M.; Devriese, L.; Godard, C.; De Herdt, P. Differentiation between High and Low Virulence Staphylococcus aureus Strains from Rabbits by Randomly Amplified Polymorphic DNA (RAPD) Analysis. Vet. Microbiol. 2000, 72, 311–319. [Google Scholar] [CrossRef]
- Ferreira, A.; Monteiro, J.M.; Vieira-Pinto, M. The Importance of Subcutaneous Abscess Infection by Pasteurella Spp. and Staphylococcus aureus as a Cause of Meat Condemnation in Slaughtered Commercial Rabbits. World Rabbit Sci. 2014, 22, 311. [Google Scholar] [CrossRef] [Green Version]
- Montanhini, M.T.M.; Bersot, L.D.S. Evaluation of Psychrotrophic Behavior and Lipolytic and Proteolytic Activity of Bacillus cereus Isolated from Refrigerated Dairy Products. Acta Sci. Technol. 2013, 35, 163–174. [Google Scholar] [CrossRef] [Green Version]
- Silva, H.O.; Lima, J.A.S.; Aguilar, C.E.G.; Rossi, G.A.M.; Mathias, L.A.; Vidal, A.M.C. Efficiency of Different Disinfectants on Bacillus cereus Sensu Stricto Biofilms on Stainless-Steel Surfaces in Contact with Milk. Front. Microbiol. 2018, 9, 2934. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Käsbohrer, A.; Schroeter, A.; Helmuth, R.; Tenhagen, B.-A. Salmonella Prevalence in Turkey Flocks before and after Implementation of the Control Program in Germany. Agriculture 2013, 3, 342–361. [Google Scholar] [CrossRef] [Green Version]
- Rezende, C.S.M.; de Mesquita, A.J.; Andrade, M.A.; Linhares, G.F.C.; de Mesquita, A.Q.; Minafra, C.S. 100_199-203. Rev. Port. Ciências Veterinárias 2005, 100, 555–556. [Google Scholar]
- Schädler, J.; Schwarz, J.; Peter-Egli, J.; Schüpbach-Regula, G.; Wiederkehr, D.; Albini, S. Survey of Salmonellae Occurrence in Meat-producing Rabbitries in Switzerland. Vet. Rec. Open 2022, 9, e24. [Google Scholar] [CrossRef]
Time | Temperature | MES (log CFU·mL−1) | SEM | p-Value | ||
---|---|---|---|---|---|---|
Time | Temp | Time × Temp | ||||
SB | 4 °C | 36.29 bB | 9.19 | 0.0397 | 0.0404 | 0.0104 |
7 °C | 54.40 aB | |||||
10 °C | 36.72 bB | |||||
SA | 4 °C | 38.69 aB | ||||
7 °C | 49.42 aB | |||||
10 °C | 88.06 aA |
Time | Treatment | EC (log CFU·mL−1) | SEM | p-Value | ||
---|---|---|---|---|---|---|
Time | Temp | Time × Temp | ||||
SB | 4 °C | 2.38 aB | 0.8962 | 0.4361 | 0.0337 | 0.7102 |
7 °C | 1.89 aB | |||||
10 °C | 2.97 aB | |||||
SA | 4 °C | 3.20 aA | ||||
7 °C | 4.15 aA | |||||
10 °C | 4.84 aA |
Time | Treatment | CPS (log CFU·mL−1) | SEM | p-Value | ||
---|---|---|---|---|---|---|
Time | Temp | Time × Temp | ||||
SB | 4 °C | 62.99 | 16.09 | 0.3918 | 0.1139 | 0.8465 |
7 °C | 53.66 | |||||
10 °C | 49.47 | |||||
SA | 4 °C | 95.11 | ||||
7 °C | 68.14 | |||||
10 °C | 67.55 |
Sample | Temperature | MES (log CFU·mL−1) | EC (log CFU·mL−1) | CPS (log CFU·mL−1) |
---|---|---|---|---|
SB | 4 °C | 0 | 0 | 0 |
7 °C | 0 | 0 | 0 | |
10 °C | 0 | 0 | 0 | |
SA | 4 °C | 8.0 | 6.66 | 2.57 |
7 °C | 13.0 | 6.07 | 7.95 | |
10 °C | 4.0 | 7.13 | 7.12 |
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Silva, J.L.M.; de Vasconcelos, M.L.; Oliveira, J.G.; da Fonseca, D.d.C.M.; Marino, E.D.; Vasconcellos, A.N.; Nascimento, L.O.; da Cruz Gomes, M.D.; Vaz, A.C.N.; de Souza, B.M.S.; et al. Effect of Different Immersion Tank Water Temperatures on the Microbiological Quality of Rabbit Carcasses. Agriculture 2023, 13, 270. https://doi.org/10.3390/agriculture13020270
Silva JLM, de Vasconcelos ML, Oliveira JG, da Fonseca DdCM, Marino ED, Vasconcellos AN, Nascimento LO, da Cruz Gomes MD, Vaz ACN, de Souza BMS, et al. Effect of Different Immersion Tank Water Temperatures on the Microbiological Quality of Rabbit Carcasses. Agriculture. 2023; 13(2):270. https://doi.org/10.3390/agriculture13020270
Chicago/Turabian StyleSilva, José Luiz Martins, Marta Liliane de Vasconcelos, Joyce Graziella Oliveira, Danielle de Cássia Martins da Fonseca, Elizangela Domenis Marino, Alenia Naliato Vasconcellos, Luciana Oliveira Nascimento, Marcia Delgado da Cruz Gomes, Andreia Cristina Nakashima Vaz, Bruna Maria Salotti de Souza, and et al. 2023. "Effect of Different Immersion Tank Water Temperatures on the Microbiological Quality of Rabbit Carcasses" Agriculture 13, no. 2: 270. https://doi.org/10.3390/agriculture13020270