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Article

Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen

1
Department of Oral and Maxillo Facial Sciences, “Sapienza” University of Rome, Via Caserta 6, 00161 Rome, Italy
2
Department of Biochemical Sciences “Alessandro Rossi Fanelli”, “Sapienza” University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
*
Authors to whom correspondence should be addressed.
J. Clin. Med. 2020, 9(4), 1099; https://doi.org/10.3390/jcm9041099
Received: 28 March 2020 / Revised: 9 April 2020 / Accepted: 10 April 2020 / Published: 12 April 2020
Liquid fibrinogen is an injectable platelet concentrate rich in platelets, leukocytes, and fibrinogen obtained by blood centrifugation. The aim of this study was to analyze the release of different growth factors in the liquid fibrinogen at different times and to assess possible correlations between growth factors and cell counts. The concentration of transforming growth factor beta 1 (TGF-β1), platelet-derived growth factor-AB (PDGF-AB), platelet-derived growth factor-BB (PDGF-BB), bone morphogenetic protein 2 (BMP-2), fibroblast growth factor 2 (FGF-2) and vascular endothelial growth factor (VEGF) released by liquid fibrinogen were examined with ELISA at three time points (T0, time of collection; T7, 7 days; T14, 14 days). The cellular content of the liquid fibrinogen and whole blood was also calculated for each volunteer. A mean accumulation of platelets of almost 1.5-fold in liquid fibrinogen compared to whole blood samples was found. An increase of TGF-β1, PDGF-AB, FGF-2, and VEGF levels was detected at T7. At T14, the level of TGF-β1 returned to T0 level; PDGF-AB amount remained high; the levels of FGF-2 and VEGF decreased with respect to T7, but remained higher than the T0 levels; PDGF-BB was high at all time points; BMP-2 level was low and remained constant at all time points. TGF-β1, PDGF-AB, and PDGF-BB showed a correlation with platelet amount, whereas BMP-2, FGF-2, and VEGF showed a mild correlation with platelet amount. Due to the high concentration of platelets, liquid fibrinogen does contain important growth factors for the regeneration of both soft and hard tissue. The centrifugation protocol tested in this study provides a valid solution to stimulate wound healing in oral and periodontal surgery. View Full-Text
Keywords: liquid fibrinogen; platelet concentrate; growth factors; platelet rich fibrin; PRF; platelets; leukocyte liquid fibrinogen; platelet concentrate; growth factors; platelet rich fibrin; PRF; platelets; leukocyte
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MDPI and ACS Style

Serafini, G.; Lopreiato, M.; Lollobrigida, M.; Lamazza, L.; Mazzucchi, G.; Fortunato, L.; Mariano, A.; Scotto d’Abusco, A.; Fontana, M.; De Biase, A. Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen. J. Clin. Med. 2020, 9, 1099. https://doi.org/10.3390/jcm9041099

AMA Style

Serafini G, Lopreiato M, Lollobrigida M, Lamazza L, Mazzucchi G, Fortunato L, Mariano A, Scotto d’Abusco A, Fontana M, De Biase A. Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen. Journal of Clinical Medicine. 2020; 9(4):1099. https://doi.org/10.3390/jcm9041099

Chicago/Turabian Style

Serafini, Giorgio, Mariangela Lopreiato, Marco Lollobrigida, Luca Lamazza, Giulia Mazzucchi, Lorenzo Fortunato, Alessia Mariano, Anna Scotto d’Abusco, Mario Fontana, and Alberto De Biase. 2020. "Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen" Journal of Clinical Medicine 9, no. 4: 1099. https://doi.org/10.3390/jcm9041099

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