Next Article in Journal
Skin Cutibacterium acnes Mediates Fermentation to Suppress the Calcium Phosphate-Induced Itching: A Butyric Acid Derivative with Potential for Uremic Pruritus
Next Article in Special Issue
Sexual Dimorphism of NAFLD in Adults. Focus on Clinical Aspects and Implications for Practice and Translational Research
Previous Article in Journal
Predictors of Mental Health Outcomes in Road Traffic Accident Survivors
Previous Article in Special Issue
Randomised Clinical Trial: Calorie Restriction Regimen with Tomato Juice Supplementation Ameliorates Oxidative Stress and Preserves a Proper Immune Surveillance Modulating Mitochondrial Bioenergetics of T-Lymphocytes in Obese Children Affected by Non-Alcoholic Fatty Liver Disease (NAFLD)
Open AccessArticle

Plasma Krebs Cycle Intermediates in Nonalcoholic Fatty Liver Disease

1
Department of Chemistry, Cleveland State University, Cleveland, OH 44115, USA
2
MetroHealth Medical Center, Case Western Reserve University, Cleveland, OH 44139, USA
3
Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44115, USA
4
Department of Gastroenterology and Hepatology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44115, USA
*
Author to whom correspondence should be addressed.
These authors have the same contribution.
J. Clin. Med. 2020, 9(2), 314; https://doi.org/10.3390/jcm9020314
Received: 9 December 2019 / Revised: 17 January 2020 / Accepted: 20 January 2020 / Published: 22 January 2020
(This article belongs to the Special Issue Should Physicians Consider NAFLD a Primary or Secondary Disease?)
Nonalcoholic liver disease (NAFLD) is manifested with a wide spectrum of clinical symptoms and is closely associated with the metabolic syndrome, inflammation, and mitochondrial dysfunction. Although the mechanism of mitochondrial dysfunction in NAFLD is still not fully elucidated, multiple studies have demonstrated evidence of molecular, biochemical, and biophysical mitochondrial abnormalities in NAFLD. Given the association between NAFLD and mitochondrial dysfunction, the aim of this study is to analyze circulating levels of Krebs cycle intermediates in a cohort of NAFLD-affected individuals and matching healthy controls and to correlate our findings with the liver function metrics. Standard serum biochemistry and Krebs cycle intermediates were analyzed in NAFLD (n = 22) and matched control (n = 67) cohorts. Circulating levels of isocitrate and citrate were significantly (p < 0.05) elevated in the NAFLD cohort of patients. The area under the curve (AUROC) for these two metabolites exhibited a moderate clinical utility. Correlations between plasma Krebs cycle intermediates and standard clinical plasma metrics were explored by Pearson’s correlation coefficient. The data obtained for plasma Krebs cycle intermediates suggest pathophysiological insights that link mitochondrial dysfunction with NAFLD. Our findings reveal that plasma isocitrate and citrate can discriminate between normal and NAFLD cohorts and can be utilized as noninvasive markers of mitochondrial dysfunction in NAFLD. Future studies with large populations at different NAFLD stages are warranted. View Full-Text
Keywords: plasma Krebs cycle intermediates; nonalcoholic fatty liver disease (NAFLD); mitochondrial dysfunction plasma Krebs cycle intermediates; nonalcoholic fatty liver disease (NAFLD); mitochondrial dysfunction
Show Figures

Figure 1

MDPI and ACS Style

Sandlers, Y.; Shah, R.R.; Pearce, R.W.; Dasarathy, J.; McCullough, A.J.; Dasarathy, S. Plasma Krebs Cycle Intermediates in Nonalcoholic Fatty Liver Disease. J. Clin. Med. 2020, 9, 314. https://doi.org/10.3390/jcm9020314

AMA Style

Sandlers Y, Shah RR, Pearce RW, Dasarathy J, McCullough AJ, Dasarathy S. Plasma Krebs Cycle Intermediates in Nonalcoholic Fatty Liver Disease. Journal of Clinical Medicine. 2020; 9(2):314. https://doi.org/10.3390/jcm9020314

Chicago/Turabian Style

Sandlers, Yana; Shah, Rohan R.; Pearce, Ryan W.; Dasarathy, Jaividhya; McCullough, Arthur J.; Dasarathy, Srinivasan. 2020. "Plasma Krebs Cycle Intermediates in Nonalcoholic Fatty Liver Disease" J. Clin. Med. 9, no. 2: 314. https://doi.org/10.3390/jcm9020314

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop