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Open AccessArticle

Parameters Affecting the Antimicrobial Properties of Cold Atmospheric Plasma Jet

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Chemistry Division, Center for General Education, Chang Gung University, Taoyuan 33302, Taiwan
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Department of Nuclear Medicine and Molecular Imaging Center, Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan
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Department of Microbiology & Immunology, Chang Gung University, Taoyuan 33302, Taiwan
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Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan
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School of Medicine, China Medical University and Hospital, Taichung 40402, Taiwan
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Department of Urology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
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Center for Plasma and Thin Film Technologies, Ming Chi University of Technology, New Taipei 24301, Taiwan
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Department of Materials Engineering, Ming Chi University of Technology, New Taipei 24301, Taiwan
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Plastic and Reconstructive Surgery, and Craniofacial Research Center, Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan
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Department of Mechanical Engineering, Chang Gung University, Taoyuan 33302, Taiwan
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Author to whom correspondence should be addressed.
J. Clin. Med. 2019, 8(11), 1930; https://doi.org/10.3390/jcm8111930 (registering DOI)
Received: 19 October 2019 / Revised: 6 November 2019 / Accepted: 7 November 2019 / Published: 9 November 2019
(This article belongs to the Special Issue Biomaterial-Related Infections)
Using the Taguchi method to narrow experimental parameters, the antimicrobial efficiency of a cold atmospheric plasma jet (CAPJ) treatment was investigated. An L9 array with four parameters of CAPJ treatments, including the application voltage, CAPJ-sample distance, argon (Ar) gas flow rate, and CAPJ treatment time, were applied to examine the antimicrobial activity against Escherichia coli (E. coli). CAPJ treatment time was found to be the most influential parameter in its antimicrobial ability by evaluation of signal to noise ratios and analysis of variance. 100% bactericidal activity was achieved under the optimal bactericidal activity parameters including the application voltage of 8.5 kV, CAPJ-sample distance of 10 mm, Ar gas flow rate of 500 sccm, and CAPJ treatment time of 300 s, which confirms the efficacy of the Taguchi method in this design. In terms of the mechanism of CAPJ’s antimicrobial ability, the intensity of hydroxyl radical produced by CAPJ positively correlated to its antimicrobial efficiency. The CAPJ antimicrobial efficiency was further evaluated by both DNA double-strand breaks analysis and scanning electron microscopy examination of CAPJ treated bacteria. CAPJ destroyed the cell wall of E. coli and further damaged its DNA structure, thus leading to successful killing of bacteria. This study suggests that optimal conditions of CPAJ can provide effective antimicrobial activity and may be grounds for a novel approach for eradicating bacterial infections. View Full-Text
Keywords: Taguchi method; antimicrobial efficiency; cold atmospheric-pressure plasma jet (CAPJ); Escherichia coli; DNA double-strand breaks; scanning electron microscopy Taguchi method; antimicrobial efficiency; cold atmospheric-pressure plasma jet (CAPJ); Escherichia coli; DNA double-strand breaks; scanning electron microscopy
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Lou, B.-S.; Lai, C.-H.; Chu, T.-P.; Hsieh, J.-H.; Chen, C.-M.; Su, Y.-M.; Hou, C.-W.; Chou, P.-Y.; Lee, J.-W. Parameters Affecting the Antimicrobial Properties of Cold Atmospheric Plasma Jet. J. Clin. Med. 2019, 8, 1930.

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