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Volume 12
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Article

Chagas Disease Diagnosis with Trypanosoma cruzi-Exclusive Epitopes in GFP

by
Andressa da M. Durans
1,2,3,
Paloma Napoleão-Pêgo
1,2,
Flavia C. G. Reis
1,
Evandro R. Dias
1,4,
Luciana E. S. F. Machado
1,5,
Guilherme C. Lechuga
1,2,
Angela C. V. Junqueira
4,
Salvatore G. De-Simone
1,2,6 and
David W. Provance, Jr.
1,2,*
1
Center for Technological Development in Health, National Institute of Science and Technology for Innovation in Neglected Population Diseases, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, Brazil
2
Epidemiology and Molecular Systematics Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, Brazil
3
Interdisciplinary Laboratory of Medical Researchers, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, Brazil
4
Laboratory on Parasitic Diseases, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, Brazil
5
Department of Genetics and Biology Evolution, Institute of Biosciences, University of São Paulo, São Paulo 05508-090, Brazil
6
Program of Post-Graduation on Science and Biotechnology, Department of Molecular and Cellular Biology, Biology Institute, Federal Fluminense University, Niterói 22040-036, Brazil
*
Author to whom correspondence should be addressed.
Vaccines 2024, 12(9), 1029; https://doi.org/10.3390/vaccines12091029
Submission received: 21 May 2024 / Revised: 11 June 2024 / Accepted: 12 June 2024 / Published: 8 September 2024
(This article belongs to the Special Issue Advances in Vaccines Against Infectious Diseases)
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Abstract

Serological tests are critical tools in the fight against infectious disease. They detect antibodies produced during an adaptive immune response against a pathogen with an immunological reagent, whose antibody binding characteristics define the specificity and sensitivity of the assay. While pathogen proteins have conveniently served as reagents, their performance is limited by the natural grouping of specific and non-specific antibody binding sites, epitopes. An attractive solution is to build synthetic proteins that only contains pathogen-specific epitopes, which could theoretically reach 100% specificity. However, the genesis of de novo proteins remains a challenge. To address the uncertainty of producing a synthetic protein, we have repurposed the beta barrel of fluorescent proteins into a receptacle that can receive several epitope sequences without compromising its ability to be expressed. Here, two versions of a multiepitope protein were built using the receptacle that differ by their grouping of epitopes specific to the parasite Trypanosoma cruzi, the causative agent for Chagas disease. An evaluation of their performance as the capture reagent in ELISAs showed near-complete agreement with recommended diagnostic protocols. The results suggest that a single assay could be developed for the diagnosis of Chagas disease and that this approach could be applied to other diseases.
Keywords: Chagas disease; Green Fluorescent Protein; Serodiagnosis; ELISA; Trypanosoma cruzi Chagas disease; Green Fluorescent Protein; Serodiagnosis; ELISA; Trypanosoma cruzi

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MDPI and ACS Style

Durans, A.d.M.; Napoleão-Pêgo, P.; Reis, F.C.G.; Dias, E.R.; Machado, L.E.S.F.; Lechuga, G.C.; Junqueira, A.C.V.; De-Simone, S.G.; Provance, D.W., Jr. Chagas Disease Diagnosis with Trypanosoma cruzi-Exclusive Epitopes in GFP. Vaccines 2024, 12, 1029. https://doi.org/10.3390/vaccines12091029

AMA Style

Durans AdM, Napoleão-Pêgo P, Reis FCG, Dias ER, Machado LESF, Lechuga GC, Junqueira ACV, De-Simone SG, Provance DW Jr. Chagas Disease Diagnosis with Trypanosoma cruzi-Exclusive Epitopes in GFP. Vaccines. 2024; 12(9):1029. https://doi.org/10.3390/vaccines12091029

Chicago/Turabian Style

Durans, Andressa da M., Paloma Napoleão-Pêgo, Flavia C. G. Reis, Evandro R. Dias, Luciana E. S. F. Machado, Guilherme C. Lechuga, Angela C. V. Junqueira, Salvatore G. De-Simone, and David W. Provance, Jr. 2024. "Chagas Disease Diagnosis with Trypanosoma cruzi-Exclusive Epitopes in GFP" Vaccines 12, no. 9: 1029. https://doi.org/10.3390/vaccines12091029

APA Style

Durans, A. d. M., Napoleão-Pêgo, P., Reis, F. C. G., Dias, E. R., Machado, L. E. S. F., Lechuga, G. C., Junqueira, A. C. V., De-Simone, S. G., & Provance, D. W., Jr. (2024). Chagas Disease Diagnosis with Trypanosoma cruzi-Exclusive Epitopes in GFP. Vaccines, 12(9), 1029. https://doi.org/10.3390/vaccines12091029

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