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Antioxidants 2018, 7(8), 104; https://doi.org/10.3390/antiox7080104

Human Lymphocyte-Protective Effects of an Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) Fruit Pulp

1
Laboratory of Applied Biochemistry and Chemistry (LABIOCA), UFR-SVT, University Ouaga 1 Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso
2
Laboratory of Animal Physiology (LPA), UFR-SVT, University Ouaga 1 Pr Joseph+ KI-ZERBO, 09 BP 848, Ouagadougou 09, Burkina Faso
*
Author to whom correspondence should be addressed.
Received: 15 July 2018 / Revised: 1 August 2018 / Accepted: 2 August 2018 / Published: 4 August 2018
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Abstract

The current study aimed to evaluate, in vitro, the antioxidant capacity and the human lymphocyte-protective effect of the ethanolic extract from Detarium microcarpum fruit pulp against oxidative stress damage. Human lymphocytes were incubated with different concentrations of extract, followed by the addition of hydrogen peroxide or tert-butyl hydroperoxide. Cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Furthermore, the antioxidant property of the extract was evaluated in vitro using hydrogen peroxide and nitric oxide radical-scavenging assays. Compared to the vehicle, the fruit pulp ethanol extract did not exhibit a cytotoxic effect on human lymphocytes. Furthermore, the cytotoxicity of hydrogen peroxide and tert-butyl hydroperoxide to human lymphocytes was significantly reduced by fruit extract pretreatment. The extract and ascorbic acid exhibited similar cytoprotective activity (p > 0.05). The fruit pulp extract showed more antioxidant activity than gallic acid in the hydrogen peroxide-scavenging model, while in the nitric oxide-quenching model, the fruit extract and gallic acid showed similar activity. The fruit pulp of D. microcarpum contains potent antioxidant and cell-protective compounds. The use of the fruit pulp of D. microcarpum as a food supplement could rescue cellular oxidative damage responsible for numerous pathologies. View Full-Text
Keywords: antioxidant; cytoprotective; hydrogen peroxide; tert-butyl hydroperoxide antioxidant; cytoprotective; hydrogen peroxide; tert-butyl hydroperoxide
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Rouamba, A.; Compaoré, M.; Ouédraogo, M.; Kiendrebeogo, M. Human Lymphocyte-Protective Effects of an Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) Fruit Pulp. Antioxidants 2018, 7, 104.

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