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Article

The Effect of the Use of the Beneficial Acetic Acid Bacteria Starter Cultures on the Microbiological and Physicochemical Quality of Raw Ripening Sausages

by
Anna Okoń
1,*,
Anna Łepecka
1,
Piotr Szymański
1 and
Katarzyna Neffe-Skocińska
2,*
1
Department of Meat and Fat Technology, Prof. Waclaw Dabrowski Institute of Agricultural and Food Biotechnology—State Research Institute, 02-532 Warsaw, Poland
2
Institute of Human Nutrition Sciences, Warsaw University of Life Sciences (WULS), Nowoursynowska St. 159C, 02-776 Warszawa, Poland
*
Authors to whom correspondence should be addressed.
Appl. Sci. 2025, 15(1), 263; https://doi.org/10.3390/app15010263
Submission received: 16 November 2024 / Revised: 27 December 2024 / Accepted: 28 December 2024 / Published: 30 December 2024
(This article belongs to the Special Issue Role of Microbes in Agriculture and Food, 2nd Edition)

Abstract

The aim of the study was to assess the possibility of using novel Gluconobacter oxydans strains in the technology of raw ripening sausages and to assess their impact on the microbiological and physico-chemical quality after production and after 6 months of storage process. Four variants of sausages were prepared: two control sausages (with salt and with curing salts addition), and two study variants with different acetic acid bacteria starters addition. Microbiological and physicochemical analyses were carried out. All variants of study sausages showed good microbiological quality concerning the total number of microorganisms, lactic acid bacteria, and the absence of pathogenic microorganisms. The synergistic effect of lactic acid bacteria and acetic acid bacteria was observed and a positive effect on the survivability of the native lactic microbiota of study sausages was shown. It was shown that sausage with the addition of acetic acid bacteria (AAB) strains were characterized by higher antioxidant properties compare to control samples. These results were confirmed by the analysis of oxidation–reduction potential and lipid oxidation products, which showed low oxidation–reduction potential (ORP value) and amounts of malondialdehyde (MDA) in the study products. Based on the obtained results of microbiological and physicochemical analysis, the technological usefulness of the study AAB strains, as starter cultures for the production of raw ripening meat products, was demonstrated.
Keywords: acetic acid bacteria (AAB); beneficial starters; meat fermentation; biopreservation process acetic acid bacteria (AAB); beneficial starters; meat fermentation; biopreservation process

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MDPI and ACS Style

Okoń, A.; Łepecka, A.; Szymański, P.; Neffe-Skocińska, K. The Effect of the Use of the Beneficial Acetic Acid Bacteria Starter Cultures on the Microbiological and Physicochemical Quality of Raw Ripening Sausages. Appl. Sci. 2025, 15, 263. https://doi.org/10.3390/app15010263

AMA Style

Okoń A, Łepecka A, Szymański P, Neffe-Skocińska K. The Effect of the Use of the Beneficial Acetic Acid Bacteria Starter Cultures on the Microbiological and Physicochemical Quality of Raw Ripening Sausages. Applied Sciences. 2025; 15(1):263. https://doi.org/10.3390/app15010263

Chicago/Turabian Style

Okoń, Anna, Anna Łepecka, Piotr Szymański, and Katarzyna Neffe-Skocińska. 2025. "The Effect of the Use of the Beneficial Acetic Acid Bacteria Starter Cultures on the Microbiological and Physicochemical Quality of Raw Ripening Sausages" Applied Sciences 15, no. 1: 263. https://doi.org/10.3390/app15010263

APA Style

Okoń, A., Łepecka, A., Szymański, P., & Neffe-Skocińska, K. (2025). The Effect of the Use of the Beneficial Acetic Acid Bacteria Starter Cultures on the Microbiological and Physicochemical Quality of Raw Ripening Sausages. Applied Sciences, 15(1), 263. https://doi.org/10.3390/app15010263

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