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Article

Healing Capacity of Bone Surrounding Biofilm-Infected and Non-Infected Gutta-Percha: A Study of Rat Calvaria

by 1,2,†, 3,†, 4, 5 and 1,2,6,*
1
Endodontics and Dental Trauma Department, School of Graduate Dentistry, Rambam Health Care Campus, P.O. Box 9602, Haifa 3109601, Israel
2
Ruth and Bruce Rappaport Faculty of Medicine, Israel Institute of Technology—Technion, Haifa 3525433, Israel
3
Department of Endodontology, The Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv 6100000, Israel
4
Department of Pathology, Rambam Health Care Campus, Haifa 3500000, Israel
5
Clinical Microbiology Laboratory, Rambam Health Care Campus, Haifa 3500000, Israel
6
The Israeli National Center for Trauma and Emergency Medicine Research Gertner Institute, Tel Hashomer 5262000, Israel
*
Author to whom correspondence should be addressed.
Equal contribution.
Academic Editor: Vittorio Checchi
Appl. Sci. 2021, 11(15), 6710; https://doi.org/10.3390/app11156710
Received: 16 June 2021 / Revised: 17 July 2021 / Accepted: 19 July 2021 / Published: 22 July 2021
(This article belongs to the Special Issue Dental Materials: Latest Advances and Prospects)
This paper aims to evaluate the healing capacity of bony lesions around biofilm-infected and non-infected gutta-percha (GP) points. Bony defects were created in the calvaria of 28 Wistar rats. The rats were divided into three groups: Group 1—Implantation of infected GP particles in the bony defect; Group 2—Positive control implantation of non-infected GP particles in the bony defect; and Group 3—Negative control, in which no GP particles were implanted. The biofilm consisted of three strains of bacteria: Enterococcus faecalis, Streptococcus sanguis, and Porphyromonas gingivalis. The animals were sacrificed 60 days postoperation, and histological assessments were performed. In Group 1, the biofilm-infected group, we observed a mild foreign body reaction with a few inflammatory cells adjacent to the capsule and a newly woven bone matrix surrounded by osteoblasts and mature bone. In Group 2, the non-infected GP particles group, minimal inflammatory cell reactions were observed in the adjacent tissue, and a newly woven bone matrix was surrounded by osteoblasts. This study shows that bone healing is possible around both sterile and infected GP points. This contradicts the claim that some root canal treatments fail because of non-microbial factors, including extruded root canal filling materials, which may cause foreign body reactions. The healing observed suggests that overextension should not be considered an indication for endodontic surgery. View Full-Text
Keywords: biofilm; bone healing capacity; infected gutta-percha; rat calvarium biofilm; bone healing capacity; infected gutta-percha; rat calvarium
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MDPI and ACS Style

Moreinos, D.; Wigler, R.; Geffen, Y.; Akrish, S.; Lin, S. Healing Capacity of Bone Surrounding Biofilm-Infected and Non-Infected Gutta-Percha: A Study of Rat Calvaria. Appl. Sci. 2021, 11, 6710. https://doi.org/10.3390/app11156710

AMA Style

Moreinos D, Wigler R, Geffen Y, Akrish S, Lin S. Healing Capacity of Bone Surrounding Biofilm-Infected and Non-Infected Gutta-Percha: A Study of Rat Calvaria. Applied Sciences. 2021; 11(15):6710. https://doi.org/10.3390/app11156710

Chicago/Turabian Style

Moreinos, Daniel, Ronald Wigler, Yuval Geffen, Sharon Akrish, and Shaul Lin. 2021. "Healing Capacity of Bone Surrounding Biofilm-Infected and Non-Infected Gutta-Percha: A Study of Rat Calvaria" Applied Sciences 11, no. 15: 6710. https://doi.org/10.3390/app11156710

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