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Extracellular Matrix Behaviour in Masseter Muscle of Patients Affected by Unilateral Posterior Crossbite: An Immunofluorescence Study
 
 
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Volume 11
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10.3390/app11125350
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Article

Use of Immunofluorescence Technique to Perform a Quantitative Analysis of Masseter Muscle Fibers in Unilateral Posterior Crossbite: A Pilot Study

by
Giovanna Vermiglio
1,
Mariagrazia Piancino
2,
Michele Runci Anastasi
3,
Giacomo Picciolo
1,
Antonio Centofanti
1,*,
Giuseppe Santoro
1,
Mariachiara Malandrino
4,
Giuseppina Cutroneo
1 and
Giuseppe Anastasi
1
1
Department of Biomedical and Dental Science and Morphofunctional Imaging, University of Messina, 98122 Messina, Italy
2
Department of Surgical Sciences, Dental School, Orthodontics, University of Turin, 10124 Torino, Italy
3
Centro Neurolesi “Bonino-Pulejo”, I.R.C.C.S (Istituto di Ricovero e Cura a Carattere Scientifico), 98100 Messina, Italy
4
School of Maxillofacial Surgery, University of Turin, 10124 Torino, Italy
*
Author to whom correspondence should be addressed.
Appl. Sci. 2021, 11(12), 5350; https://doi.org/10.3390/app11125350
Submission received: 12 May 2021 / Revised: 4 June 2021 / Accepted: 7 June 2021 / Published: 9 June 2021
(This article belongs to the Special Issue Immunohistochemical Expression Volume II)
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Abstract

Unilateral posterior crossbite is a type of malocclusion that involves morpho-functional characteristics of masticatory muscle, such as the masseter: electrophysiological data have shown that the affected side works less than the contralateral muscle, which shows a normal or increased activity, probably in order to compensate for the affected side. The aim of present work was to measure the diameter and the cross-sectional area of ipsilateral and contralateral muscle fibers to verify if hypertrophy and/or hypotrophy take place in this malocclusion. We used immunofluorescence pictures to measure, using ImageJ software, the diameter and the cross-sectional area of fibers from control and crossbite groups; after that, the data were processed to perform statistical analyses. Results show that the fiber diameters of contralateral muscle are larger than the diameters of ipsilateral and control fibers, and that this difference is statistically significant. No statistically significant difference was found between the fiber diameters of the ipsilateral and control muscles. All these data suggest that, during unilateral posterior crossbite, morphological changes take place in the contralateral masseter muscle, which undergoes hypertrophy, probably to compensate for the low activity of the affected muscle.
Keywords: masseter muscle; crossbite; ImageJ; immunofluorescence; fiber size masseter muscle; crossbite; ImageJ; immunofluorescence; fiber size

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MDPI and ACS Style

Vermiglio, G.; Piancino, M.; Runci Anastasi, M.; Picciolo, G.; Centofanti, A.; Santoro, G.; Malandrino, M.; Cutroneo, G.; Anastasi, G. Use of Immunofluorescence Technique to Perform a Quantitative Analysis of Masseter Muscle Fibers in Unilateral Posterior Crossbite: A Pilot Study. Appl. Sci. 2021, 11, 5350. https://doi.org/10.3390/app11125350

AMA Style

Vermiglio G, Piancino M, Runci Anastasi M, Picciolo G, Centofanti A, Santoro G, Malandrino M, Cutroneo G, Anastasi G. Use of Immunofluorescence Technique to Perform a Quantitative Analysis of Masseter Muscle Fibers in Unilateral Posterior Crossbite: A Pilot Study. Applied Sciences. 2021; 11(12):5350. https://doi.org/10.3390/app11125350

Chicago/Turabian Style

Vermiglio, Giovanna, Mariagrazia Piancino, Michele Runci Anastasi, Giacomo Picciolo, Antonio Centofanti, Giuseppe Santoro, Mariachiara Malandrino, Giuseppina Cutroneo, and Giuseppe Anastasi. 2021. "Use of Immunofluorescence Technique to Perform a Quantitative Analysis of Masseter Muscle Fibers in Unilateral Posterior Crossbite: A Pilot Study" Applied Sciences 11, no. 12: 5350. https://doi.org/10.3390/app11125350

APA Style

Vermiglio, G., Piancino, M., Runci Anastasi, M., Picciolo, G., Centofanti, A., Santoro, G., Malandrino, M., Cutroneo, G., & Anastasi, G. (2021). Use of Immunofluorescence Technique to Perform a Quantitative Analysis of Masseter Muscle Fibers in Unilateral Posterior Crossbite: A Pilot Study. Applied Sciences, 11(12), 5350. https://doi.org/10.3390/app11125350

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