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Ameliorative Effect of Bacillus subtilis on Growth Performance and Intestinal Architecture in Broiler Infected with Salmonella

Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia
Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt
Department of Poultry Production, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 333516, Egypt
Author to whom correspondence should be addressed.
Animals 2019, 9(4), 190;
Received: 6 March 2019 / Revised: 9 April 2019 / Accepted: 12 April 2019 / Published: 23 April 2019
(This article belongs to the Special Issue Poultry Nutrition)
PDF [211 KB, uploaded 23 April 2019]

Simple Summary

Salmonellosis is a dangerous disease in broilers that causes huge economic losses. We assumed that instead of antibiotics, a Bacillus-based probiotic may serve as an alternative to alleviate the negative effects of Salmonella infection. A control group with no feed additive, a positive control supplemented with a standard antibiotic and two groups that were supplemented with different strains and levels of Bacillus subtilis were the experimental animals of the present study. It was revealed that supplementation of probiotic bacteria induced similar results in terms of feed intake, body weight gain and feed efficiency in comparison with the group treated with antibiotics. In addition, the dimensions of intestinal villi were also improved in the probiotic-treated birds. As concluded from the results of the present study, probiotic bacteria could be used as an alternative to antibiotics against Salmonella in broilers.


A total of 600 day-old broiler chicks (Ross 308) confirmed for the absence of Salmonella were randomly allocated to five treatments each with 10 replicates: negative control (basal diet only); positive control (basal diet) + infected with Salmonella; T1, Salmonella infected + avilamycin; T2, Salmonella infected + Bacillus subtilis (ATCC PTA-6737; 2 × 107 CFU/g) and T3, Salmonella infected + B. subtilis (DSM 172999; 1.2 × 106 CFU/g). The results revealed that feed intake (FI) and body weight (BW) were significantly (p < 0.01) lower in T1 compared to T2. The feed conversion ratio (FCR) was significantly (p < 0.01) lower in T2 and T3 compared to other treatments. Similarly, the performance efficiency factor (PEF) was also significantly (p < 0.01) higher in T2 and T3 compared to positive control. Villus height was significantly (p < 0.01) higher in T2 compared to all other treatments. However, villus width and surface area were significantly (p < 0.01) higher in T1. In conclusion, dietary supplementation with B. subtilis improved growth and intestinal health by reversing the negative effects of Salmonellosis. View Full-Text
Keywords: broiler; growth; intestinal villi; Salmonella broiler; growth; intestinal villi; Salmonella
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Abudabos, A.M.; Ali, M.H.; Nassan, M.A.; Saleh, A.A. Ameliorative Effect of Bacillus subtilis on Growth Performance and Intestinal Architecture in Broiler Infected with Salmonella. Animals 2019, 9, 190.

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