The Effects of a Glucocorticoid Receptor Agonist (GRA) on the Immune Function, Nutrient Digestibility, and Wean-to-Finish Growth Performance of Early-Weaned Pigs
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Treatments
2.2. Sample Collection
2.3. Sample Processing and Analysis
2.4. Statistical Analysis
3. Results
3.1. Complete Blood Cell Count and Blood Chemistry
3.2. Histomorphology of the Intestinal Lining, Intestinal Enzymes, and Nutrient Digestibility
3.3. Growth Performance
3.3.1. Nursery Period (0 to 28 Days Post-Weaning)
3.3.2. Grow-to-Finish Phase (49 to 126 Days Post-Weaning)
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
- Moeser, A.J.; Klok, C.V.; Ryan, K.A.; Wooten, J.G.; Little, D.; Cook, V.L.; Blikslager, A.T. Stress signaling pathways activated by weaning mediate intestinal dysfunction in the pig. Am. J. Physiol. Gastrointest. Liver Physiol. 2007, 292, G173–G181. [Google Scholar] [CrossRef] [PubMed]
- Campbell, J.M.; Crenshaw, J.D.; Polo, J. The biological stress of early weaned piglets. J. Anim. Sci. Biotechnol. 2013, 4, 19. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Wooten, H.; McGlone, J.J.; Wachtel, M.; Thompson, G.; Rakhshandeh, A.R.; Rakhshandeh, A. A glucocorticoid receptor agonist improves post-weaning growth performance in segregated early-weaned pigs. Anim. Int. J. Anim. Biosci. 2019, 13, 1972–1981. [Google Scholar] [CrossRef] [PubMed]
- Pluske, J.R.; Dividich, J.L.; Verstegen, M.W.A. Weaning the Pig: Concepts and Consequences; Academic Publishers: Wageningen, The Netherlands, 2003; ISBN 978-90-76998-17-6. [Google Scholar]
- Kats, L.J.; Tokach, M.D.; Goodband, R.D.; Nelssen, J.L. Influence of Weaning Weight and Growth During the First Week Postweaning on Subsequent Pig Performance; Agricultural Experiment Station and Cooperative Extension Service; Kansas State University: Manhattan, KS, USA, 1992; pp. 15–17. [Google Scholar]
- Schinckel, A.P.; Richert, B.T.; Sholly, D.M.; Radcliffe, J.S.; Sutton, A.L.; Einstein, M.E. Impact of Weaning Weight and Early Postweaning Growth of Pigs to Late Finishing Growth When Fed Either Corn- and Soybean Meal-Based Diets or Low Nutrient Excretion Nutrient-Dense Diets. Prof. Anim. Sci. 2009, 25, 175–188. [Google Scholar] [CrossRef]
- Collins, C.L.; Pluske, J.R.; Morrison, R.S.; McDonald, T.N.; Smits, R.J.; Henman, D.J.; Stensland, I.; Dunshea, F.R. Post-weaning and whole-of-life performance of pigs is determined by live weight at weaning and the complexity of the diet fed after weaning. Anim. Nutr. Zhongguo Xu Mu Shou Yi Xue Hui 2017, 3, 372–379. [Google Scholar] [CrossRef]
- Conrad, M.S.; Sutton, B.P.; Larsen, R.; Van Alstine, W.G.; Johnson, R.W. Early postnatal respiratory viral infection induces structural and neurochemical changes in the neonatal piglet brain. Brain. Behav. Immun. 2015, 48, 326–335. [Google Scholar] [CrossRef] [Green Version]
- Moeser, A.J.; Pohl, C.S.; Rajput, M. Weaning stress and gastrointestinal barrier development: Implications for lifelong gut health in pigs. Anim. Nutr. Zhongguo Xu Mu Shou Yi Xue Hui 2017, 3, 313–321. [Google Scholar] [CrossRef]
- Dibner, J.J.; Richards, J.D. Antibiotic growth promoters in agriculture: History and mode of action. Poult. Sci. 2005, 84, 634–643. [Google Scholar] [CrossRef]
- Niewold, T.A. Mechanisms of Antibiotics: How Do They Really Work. Adv. Pork Prod. 2013, 24, 103–106. [Google Scholar]
- McBride, W.D.; Key, N.; Mathews, K.H. Subtherapeutic Antibiotics and Productivity in U.S. Hog Production. Appl. Econ. Perspect. Policy 2008, 30, 270–288. [Google Scholar] [CrossRef]
- Sneeringer, S.; MacDonald, J.M.; Key, N.; McBride, W.D.; Mathews, K. Economics of Antibiotic Use in U.S. Livestock Production; Social Science Research Network: Rochester, NY, USA, 2017. [Google Scholar]
- Robert, S.; Passillé, A.-M.B.D.; St-Pierre, N.; Pelletier, G.; Petitclerc, D.; Dubreuil, P.; Brazeau, P. Effect of the Stress of Injections on the Serum Concentration of Cortisol, Prolactin, and Growth Hormone in Gilts and Lactating Sows. Can. J. Anim. Sci. 1989, 69, 663–672. [Google Scholar] [CrossRef]
- Donkoh, A.; Moughan, P.J.; Smith, W.C. Comparison of the slaughter method and simple T-piece cannulation of the terminal ileum for determining ileal amino acid digestibility in meat and bone meal for the growing pig. Anim. Feed Sci. Technol. 1994, 49, 43–56. [Google Scholar] [CrossRef]
- Hu, C.H.; Xiao, K.; Luan, Z.S.; Song, J. Early weaning increases intestinal permeability, alters expression of cytokine and tight junction proteins, and activates mitogen-activated protein kinases in pigs. J. Anim. Sci. 2013, 91, 1094–1101. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- McGilvray, W.D.; Klein, D.; Wooten, H.; Dawson, J.A.; Hewitt, D.; Rakhshandeh, A.R.; De Lange, C.F.M.; Rakhshandeh, A. Immune system stimulation induced by Escherichia coli lipopolysaccharide alters plasma free amino acid flux and dietary nitrogen utilization in growing pigs. J. Anim. Sci. 2019, 97, 315–326. [Google Scholar] [CrossRef]
- Official Methods of Analysis, 16th ed.; AOAC: Gaithersburg, MD, USA, 1997.
- Pié, S.; Lallès, J.P.; Blazy, F.; Laffitte, J.; Sève, B.; Oswald, I.P. Weaning Is Associated with an Upregulation of Expression of Inflammatory Cytokines in the Intestine of Piglets. J. Nutr. 2004, 134, 641–647. [Google Scholar] [CrossRef] [Green Version]
- Stokes, C.R.; Bailey, M.; Haverson, K.; Harris, C.; Jones, P.; Inman, C.; Pié, S.; Oswald, I.P.; Williams, B.A.; Akkermans, A.D.L.; et al. Postnatal development of intestinal immune system in piglets: Implications for the process of weaning. Anim. Res. 2004, 53, 325–334. [Google Scholar] [CrossRef] [Green Version]
- Smith, F.; Clark, J.E.; Overman, B.L.; Tozel, C.C.; Huang, J.H.; Rivier, J.E.F.; Blisklager, A.T.; Moeser, A.J. Early weaning stress impairs development of mucosal barrier function in the porcine intestine. Am. J. Physiol.-Gastrointest. Liver Physiol. 2009, 298, G352–G363. [Google Scholar] [CrossRef] [Green Version]
- Medland, J.E.; Pohl, C.S.; Edwards, L.L.; Frandsen, S.; Bagley, K.; Li, Y.; Moeser, A.J. Early life adversity in piglets induces long-term upregulation of the enteric cholinergic nervous system and heightened, sex-specific secretomotor neuron responses. Neurogastroenterol. Motil. 2016, 28, 1317–1329. [Google Scholar] [CrossRef] [Green Version]
- Wyns, H.; Meyer, E.; Watteyn, A.; Plessers, E.; De Baere, S.; De Backer, P.; Croubels, S. Pharmacokinetics of dexamethasone after intravenous and intramuscular administration in pigs. Vet. J. 2013, 198, 286–288. [Google Scholar] [CrossRef] [Green Version]
- Sugiharto, S.; Hedemann, M.S.; Lauridsen, C. Plasma metabolomic profiles and immune responses of piglets after weaning and challenge with E. coli. J. Anim. Sci. Biotechnol. 2014, 5, 17. [Google Scholar] [CrossRef] [Green Version]
- Ronchetti, S.; Ricci, E.; Migliorati, G.; Gentili, M.; Riccardi, C. How Glucocorticoids Affect the Neutrophil Life. Int. J. Mol. Sci. 2018, 19, 4090. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Coutinho, A.E.; Chapman, K.E. The anti-inflammatory and immunosuppressive effects of glucocorticoids, recent developments and mechanistic insights. Mol. Cell. Endocrinol. 2011, 335, 2–13. [Google Scholar] [CrossRef] [PubMed]
- Praituan, W.; Rojnuckarin, P. Faster platelet recovery by high-dose dexamethasone compared with standard-dose prednisolone in adult immune thrombocytopenia: A prospective randomized trial. J. Thromb. Haemost. 2009, 7, 1036–1038. [Google Scholar] [CrossRef] [PubMed]
- Borges, T.J.; Wieten, L.; van Herwijnen, M.J.C.; Broere, F.; Van Der Zee, R.; Bonorino, C.; Van Eden, W. The anti-inflammatory mechanisms of Hsp70. Front. Immunol. 2012, 3. [Google Scholar] [CrossRef] [Green Version]
- Wang, Y.; Lin, F.; Zhu, X.; Leone, V.A.; Dalal, S.; Tao, Y.; Messer, J.S.; Chang, E.B. Distinct roles of intracellular heat shock protein 70 in maintaining gastrointestinal homeostasis. Am. J. Physiol.-Gastrointest. Liver Physiol. 2017, 314, G164–G178. [Google Scholar] [CrossRef]
- Zhang, M.; Yue, Z.; Liu, Z.; Islam, A.; Rehana, B.; Tang, S.; Bao, E.; Hartung, J. Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times. J. Vet. Sci. 2012, 13, 253–259. [Google Scholar] [CrossRef] [Green Version]
- Estaki, M.; DeCoffe, D.; Gibson, D.L. Interplay between intestinal alkaline phosphatase, diet, gut microbes and immunity. World J. Gastroenterol. WJG 2014, 20, 15650–15656. [Google Scholar] [CrossRef]
- Alam, S.N.; Yammine, H.; Moaven, O.; Ahmed, R.; Moss, A.K.; Biswas, B.; Muhammad, N.; Biswas, R.; Raychowdhury, A.; Kaliannan, K.; et al. Intestinal Alkaline Phosphatase Prevents Antibiotic-Induced Susceptibility to Enteric Pathogens. Ann. Surg. 2014, 259, 715–722. [Google Scholar] [CrossRef]
- Wiedmeyer, C.E.; Solter, P.F.; Hoffmann, W.E. Alkaline phosphatase expression in tissues from glucocorticoid-treated dogs. Am. J. Vet. Res. 2002, 63, 1083–1088. [Google Scholar] [CrossRef]
- Lackeyram, D.; Yang, C.; Archbold, T.; Swanson, K.C.; Fan, M.Z. Early Weaning Reduces Small Intestinal Alkaline Phosphatase Expression in Pigs. J. Nutr. 2010, 140, 461–468. [Google Scholar] [CrossRef] [Green Version]
- Hosten, A.O. BUN and Creatinine. In Clinical Methods: The History, Physical, and Laboratory Examinations; Walker, H.K., Hall, W.D., Hurst, J.W., Eds.; Butterworths: Boston, MA, USA, 1990; ISBN 978-0-409-90077-4. [Google Scholar]
- Sterndale, S.O.; Miller, D.W.; Mansfield, J.P.; Kim, J.C.; Pluske, J.R. Corticosteroid administration can mitigate the stress response of post-weaned pigs exposed to mixing. Adv. Anim. Biosci. Manip. Pig Prod. XVII 2019, 10, s44. [Google Scholar] [CrossRef] [Green Version]
- De Jm, K.; Aa, W. Incidence of chronic inflammations in gilts and castrated boars. Tijdschr. Diergeneeskd. 1988, 113, 415–417. [Google Scholar]
- Rehman, K.; Akash, M.S.H. Mechanisms of inflammatory responses and development of insulin resistance: How are they interlinked? J. Biomed. Sci. 2016, 23, 87. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ruis, M.A.W.; Te Brake, J.H.A.; Engel, B.; Ekkel, E.D.; Buist, W.G.; Blokhuis, H.J.; Koolhaas, J.M. The Circadian Rhythm of Salivary Cortisol in Growing Pigs: Effects of Age, Gender, and Stress. Physiol. Behav. 1997, 62, 623–630. [Google Scholar] [CrossRef]
- National Research Council. Nutrient Requirements of Swine: Eleventh Revised Edition, 11th ed.; National Academic Press: Washington, DC, USA, 2012; ISBN 978-0-309-48903-4. [Google Scholar]
- Kim, Y.S.; Ho, S.B. Intestinal Goblet Cells and Mucins in Health and Disease: Recent Insights and Progress. Curr. Gastroenterol. Rep. 2010, 12, 319–330. [Google Scholar] [CrossRef] [Green Version]
- Leppkes, M.; Roulis, M.; Neurath, M.F.; Kollias, G.; Becker, C. Pleiotropic functions of TNF-α in the regulation of the intestinal epithelial response to inflammation. Int. Immunol. 2014, 26, 509–515. [Google Scholar] [CrossRef]
- Mangos, G.J.; Whitworth, J.A.; Williamson, P.M.; Kelly, J.J. Glucocorticoids and the kidney. Nephrology 2003, 8, 267–273. [Google Scholar] [CrossRef]
- Kem, D.C.; Gomez-Sanchez, C.; Kramer, N.J.; Holland, O.B.; Higgins, J.R. Plasma Aldosterone and Renin Activity Response to ACTH Infusion in Dexamethasone-Suppressed Normal and Sodium-Depleted Man. J. Clin. Endocrinol. Metab. 1975, 40, 116–124. [Google Scholar] [CrossRef]
- Bruininx, E.M.; van der Peet-Schwering, C.M.C.; Schrama, J.W.; Vereijken, P.F.G.; Vesseur, P.C.; Everts, H.; den Hartog, L.A.; Beynen, A.C. Individually measured feed intake characteristics and growth performance of group-housed weanling pigs: Effects of sex, initial body weight, and body weight distribution within groups. J. Anim. Sci. 2001, 79, 301–308. [Google Scholar] [CrossRef]
- Hyun, Y.; Ellis, M. Effect of group size and feeder type on growth performance and feeding patterns in growing pigs. J. Anim. Sci. 2001, 79, 803–810. [Google Scholar] [CrossRef] [Green Version]
Ingredient and Nutrient Contents | Diets 2 | ||
---|---|---|---|
Phase 1 | Phase 2 | Phase 3 | |
Ingredient, % (as fed) | |||
Corn | 33.75 | 42.91 | 60.65 |
SBM | 22 | 29.15 | 34 |
Whey powder | 27.5 | 17.59 | - |
Plasma powder | 4 | 3.02 | - |
Fish meal | 4.5 | - | - |
Fat | 2 | 1.51 | 1 |
Dicalcium phosphate | 0.4 | 1.51 | 1.4 |
Calcium carbonate | 0.7 | 0.7 | 0.7 |
Salt | 0.45 | 0.35 | 0.25 |
Swine premix 1 | 4 | 3 | 2 |
Zinc oxide | 0.3 | 0.25 | - |
TiO2 | 0.4 | 0.3 | 0.25 |
Calculated nutrient content, g/kg | |||
ME, MJ/kg | 14 | 14.1 | 13.7 |
CP (N × 6.25) | 206 | 193 | 181 |
SID Lys 3 | 13.6 | 12 | 10.1 |
Ca | 8.2 | 8.6 | 7.4 |
STTD P | 5 | 5.2 | 3.8 |
Ca:STTD P | 1.64 | 1.65 | 1.95 |
Measurement | GRA− | GRA+ | p≤ | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ANT− | ANT+ | ANT− | ANT+ | SE | GRA | ANT | SEX | GRA × ANT | GRA × SEX | ANT × SEX | GRA × ANT × SEX | |||||
Gilt | Barrow | Gilt | Barrow | Gilt | Barrow | Gilt | Barrow | |||||||||
Total WBC (K/μL) | 18.5 | 16.6 | 15.1 | 19.9 | 13.5 | 14.1 | 14.2 | 16.8 | 1.63 | 0.01 | 0.41 | 0.13 | 0.38 | 0.92 | 0.03 | 0.25 |
Neutrophils (K/μL) | 9.4 | 8.7 | 9.5 | 12.6 | 7.7 | 8.7 | 12.1 | 9.3 | 4.20 | 0.7 | 0.16 | 0.91 | 0.86 | 0.51 | 0.99 | 0.23 |
Lymphocytes (K/μL) | 6.4 | 6.4 | 4.8 | 5.8 | 5.5 | 4.8 | 5.8 | 5.9 | 0.80 | 0.45 | 0.59 | 0.75 | 0.03 | 0.35 | 0.3 | 0.91 |
N:L ratio (K/μL) | 1.6 | 1.5 | 2.1 | 2.1 | 1.9 | 2.0 | 2.5 | 2 | 0.82 | 0.5 | 0.22 | 0.70 | 0.70 | 0.80 | 0.73 | 0.62 |
Monocytes (K/μL) | 0.7 | 0.4 | 0.9 | 0.9 | 0.6 | 0.5 | 0.7 | 0.9 | 0.21 | 0.63 | 0.01 | 0.68 | 0.51 | 0.27 | 0.18 | 0.80 |
Eosinophils (K/μL) | 1.0 | 0.7 | 0.5 | 0.4 | 0.3 | 0.5 | 0.5 | 0.5 | 0.12 | 0.01 | 0.01 | 0.77 | 0.01 | 0.06 | 0.68 | 0.13 |
RBC (M/μL) | 6.8 | 6.8 | 7.1 | 7.1 | 7.2 | 7.1 | 6.8 | 6.9 | 0.24 | 0.69 | 0.91 | 0.73 | 0.07 | 0.93 | 0.62 | 0.46 |
Hemoglobin (g/dL) | 10.9 | 10.3 | 10.7 | 10.8 | 10.8 | 10.6 | 9.4 | 10.2 | 0.77 | 0.42 | 0.50 | 0.96 | 0.28 | 0.57 | 0.38 | 0.91 |
Hematocrit (%) | 38.7 | 37.4 | 38.8 | 39.1 | 38.7 | 39 | 34.7 | 37.4 | 3.04 | 0.56 | 0.59 | 0.78 | 0.31 | 0.6 | 0.58 | 0.92 |
Platelets (K/μL) | 705.5 | 679.6 | 767.6 | 758.4 | 931.6 | 885.5 | 726.3 | 887.5 | 151.22 | 0.05 | 0.81 | 0.76 | 0.19 | 0.57 | 0.39 | 0.47 |
Plasma protein (g/dL) | 6.1 | 5.9 | 5.4 | 5.8 | 5.4 | 4.8 | 6.1 | 5.6 | 0.24 | 0.03 | 0.12 | 0.13 | 0.01 | 0.03 | 0.25 | 0.35 |
Fibrinogen (mg/dL) | 700.3 | 647.2 | 578.3 | 572.1 | 550.3 | 578.9 | 577 | 574.7 | 40.83 | 0.03 | 0.08 | 0.73 | 0.03 | 0.38 | 0.87 | 0.42 |
Electrolyte Balance (mEq/L) | 37.9 | 38.5 | 39 | 38.2 | 39.2 | 38.5 | 38.1 | 38 | 1.25 | 0.95 | 0.66 | 0.69 | 0.26 | 0.86 | 0.66 | 0.32 |
Glucose (mg/dL) | 114.9 | 128.8 | 117.7 | 124.9 | 124.6 | 119 | 111.1 | 130.1 | 9.14 | 0.93 | 0.83 | 0.04 | 0.94 | 0.64 | 0.28 | 0.06 |
BUN (mg/dL) | 7.4 | 8.3 | 8.1 | 6.7 | 5.7 | 5.6 | 6.9 | 5.0 | 1.39 | 0.04 | 0.95 | 0.48 | 0.67 | 0.69 | 0.26 | 0.92 |
Creatinine (mg/dL) | 1.26 | 1.23 | 1.24 | 1.19 | 1.09 | 1.15 | 1.08 | 1.14 | 0.095 | 0.02 | 0.68 | 0.8 | 0.81 | 0.31 | 0.9 | 0.93 |
Anion gap (mmol/L) | 16.8 | 17.6 | 17 | 17.3 | 17.3 | 16.8 | 17.1 | 17.7 | 0.74 | 0.92 | 0.78 | 0.46 | 0.59 | 0.54 | 0.7 | 0.32 |
Measurement | GRA− | GRA+ | p≤ | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ANT− | ANT+ | ANT− | ANT+ | SE | GRA | ANT | SEX | GRA × ANT | GRA × SEX | ANT × SEX | GRA × ANT × SEX | |||||
Gilt | Barrow | Gilt | Barrow | Gilt | Barrow | Gilt | Barrow | |||||||||
Mucosal morphology | ||||||||||||||||
Villus height (mm) | 0.26 | 0.26 | 0.28 | 0.29 | 0.25 | 0.23 | 0.26 | 0.23 | 0.013 | 0.01 | 0.03 | 0.23 | 0.3 | 0.04 | 0.99 | 0.84 |
Crypt depth (mm) | 0.23 | 0.24 | 0.25 | 0.25 | 0.27 | 0.25 | 0.25 | 0.23 | 0.012 | 0.31 | 0.86 | 0.81 | 0.03 | 0.23 | 0.9 | 0.84 |
Villus density (villi/mm) | 9.08 | 8.69 | 9.48 | 9.12 | 9.83 | 9.6 | 9.13 | 9.40 | 0.338 | 0.07 | 0.93 | 0.42 | 0.05 | 0.37 | 0.53 | 0.59 |
VH:CD | 1.18 | 1.14 | 1.19 | 1.22 | 1.01 | 0.97 | 1.13 | 1.07 | 0.078 | 0.01 | 0.12 | 0.53 | 0.50 | 0.71 | 0.8 | 0.66 |
Jejunum enzymes | ||||||||||||||||
SI (ng/mg) | 117 a | 107 a,b,c | 104 b,c | 104 bc | 105 b,c | 113 a,b | 107 a,b,c | 103 b,c | 5.10 | 0.77 | 0.17 | 0.37 | 0.61 | 0.09 | 0.88 | 0.01 |
MGA (ng/mg) | 6.5 | 6.5 | 7.0 | 7.0 | 8.1 | 8.1 | 7.5 | 7.0 | 1.26 | 0.31 | 0.86 | 0.17 | 0.43 | 0.23 | 0.15 | 0.13 |
APEP (pg/mg) | 105 | 100 | 174 | 150 | 106 | 117 | 137 | 131 | 14.5 | 0.29 | 0.01 | 0.30 | 0.04 | 0.14 | 0.14 | 0.89 |
AP (ng/mg) | 17.0 | 21.1 | 13.5 | 12.1 | 17.3 | 20.0 | 12.3 | 12.1 | 1.84 | 0.69 | 0.01 | 0.04 | 0.94 | 0.96 | 0.01 | 0.30 |
HSP-70 (ng/mg) | 52.3 b | 52.8 b | 55.0 b | 16.9 b | 92.3 a | 94.3 a | ND | ND | 20.19 | 0.81 | 0.01 | 0.94 | 0.01 | 0.02 | 0.93 | 0.02 |
Digestibility | ||||||||||||||||
ATTD of energy | 0.57 | 0.56 | 0.57 | 0.59 | 0.64 | 0.63 | 0.63 | 0.63 | 0.047 | 0.01 | 0.86 | 0.96 | 0.45 | 0.79 | 0.6 | 0.86 |
AID of protein | 0.74 | 0.77 | 0.84 | 0.89 | 0.93 | 0.89 | 0.77 | 0.60 | 0.059 | 0.7 | 0.11 | 0.49 | 0.01 | 0.11 | 0.34 | 0.22 |
Measurement | GRA− | GRA+ | p≤ | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ANT− | ANT+ | ANT− | ANT+ | SE | GRA | ANT | SEX | GRA × ANT | GRA × SEX | ANT × SEX | GRA × ANT × SEX | |||||
Gilt | Barrow | Gilt | Barrow | Gilt | Barrow | Gilt | Barrow | |||||||||
ADG, kg/d | ||||||||||||||||
Overall | 0.28 | 0.25 | 0.35 | 0.34 | 0.41 | 0.31 | 0.33 | 0.33 | 0.033 | 0.02 | 0.17 | 0.05 | 0.01 | 0.32 | 0.08 | 0.38 |
Day 7 | −0.02 z | 0.04 z | 0.12 z | 0.12 z | 0.21 y | 0.11 y | 0.13 z | 0.06 y | 0.066 | - | - | - | - | - | - | - |
Day 14 | 0.24 y | 0.16 y | 0.30 y | 0.29 y | 0.33 y | 0.33 x | 0.25 y,z | 0.37 x | 0.065 | - | - | - | - | - | - | - |
Day 21 | 0.51 x | 0.36 x | 0.45 x | 0.45 x,y | 0.49 x | 0.41 x | 0.56 x | 0.43 x | 0.050 | - | - | - | - | - | - | - |
Day 28 | 0.40 x,y | 0.43 x | 0.50 x | 0.52 x | 0.62 x | 0.39 x | 0.39 x,y | 0.45 x | 0.060 | - | - | - | - | - | - | - |
BW, kg | ||||||||||||||||
Overall | 8.9 | 8.9 | 9.9 | 9.8 | 10.3 | 9.7 | 9.8 | 9.6 | 0.23 | 0.01 | 0.04 | 0.11 | 0.01 | 0.27 | 0.51 | 0.40 |
Day 7 | 7.5 u | 8.0 z | 8.5 u | 8.5 u | 9.1 u | 8.5 u | 8.6 u | 8.1 u | 0.43 | - | - | - | - | - | - | - |
Day 14 | 9.2 z | 9.0 z | 10.6 z | 10.5 z | 11.4 z | 10.8 z | 10.3 z | 10.6 z | 0.35 | - | - | - | - | - | - | - |
Day 21 | 12.8 y | 11.5 y | 13.8 y | 13.7 y | 14.8 y | 13.6 y | 14.1 y | 13.6 y | 0.44 | - | - | - | - | - | - | - |
Day 28 | 15.7 x | 14.5 x | 17.3 x | 17.3 x | 19.2 x | 16.3 x | 16.9 x | 16.8 x | 0.43 | - | - | - | - | - | - | - |
ADFI, kg/d | ||||||||||||||||
Overall | 0.58 | 0.49 | 0.61 | 0.59 | 0.63 | 0.52 | 0.53 | 0.57 | 0.047 | 0.89 | 0.53 | 0.18 | 0.21 | 0.71 | 0.16 | 0.60 |
Day 7 | 0.16 u | 0.18 u | 0.20 u | 0.22 u | 0.23 u | 0.18 u | 0.16 z | 0.23 u | 0.060 | - | - | - | - | - | - | - |
Day 14 | 0.46 z | 0.40 z | 0.44 z | 0.44 z | 0.47 z | 0.43 z | 0.33 y | 0.45 z | 0.059 | - | - | - | - | - | - | - |
Day 21 | 0.75 y | 0.55 y | 0.73 y | 0.72 y | 0.77 y | 0.66 y | 0.75 x | 0.70 y | 0.064 | - | - | - | - | - | - | - |
Day 28 | 0.96 x | 0.84 x | 1.06 x | 0.97 x | 1.03 x | 0.83 x | 0.89 x | 0.91 x | 0.063 | - | - | - | - | - | - | - |
G:F | ||||||||||||||||
Overall | 0.35 | 0.38 | 0.56 | 0.60 | 0.63 | 0.61 | 0.64 | 0.51 | 0.078 | 0.01 | 0.05 | 0.68 | 0.01 | 0.20 | 0.55 | 0.52 |
Day 7 | −0.1 z | 0.19 z | 0.59 x,y | 0.71 x | 0.75 x | 0.63 x,y | 0.73 x | 0.22 y | 0.120 | - | - | - | - | - | - | - |
Day 14 | 0.51 x,y | 0.40 x,y,z | 0.65 x | 0.68 x | 0.72 x | 0.79 x | 0.72 x | 0.84 x | 0.150 | - | - | - | - | - | - | - |
Day 21 | 0.68 x | 0.64 x | 0.67 x | 0.65 x,y | 0.64 x,y | 0.64 x,y | 0.74 x | 0.62 x,y | 0.119 | - | - | - | - | - | - | - |
Day 28 | 0.28 y | 0.29 yz | 0.33 y | 0.37 y | 0.40 y | 0.38 y | 0.37 y | 0.36 y | 0.153 | - | - | - | - | - | - | - |
Measurement | GRA− | GRA+ | p≤ | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ANT− | ANT+ | ANT− | ANT+ | SE | GRA | ANT | SEX | GRA × ANT | GRA × SEX | ANT × SEX | GRA × ANT × SEX | |||||
Gilt | Barrow | Gilt | Barrow | Gilt | Barrow | Gilt | Barrow | |||||||||
ADG, kg/d | ||||||||||||||||
Overall | 0.84 | 0.89 | 0.84 | 0.90 | 0.85 | 0.90 | 0.90 | 0.95 | 0.051 | 0.20 | 0.27 | 0.04 | 0.31 | 0.84 | 0.97 | 0.95 |
Day 49 | 0.58 z | 0.60 z | 0.62 y | 0.66 y | 0.60 z | 0.60 z | 0.65 u | 0.60 u | 0.069 | - | - | - | - | - | - | - |
Day 63 | 0.91 x | 0.74 y,z | 0.99 x | 0.80 y | 0.83 y | 0.76 y,z | 0.83 z | 0.96 z | 0.110 | - | - | - | - | - | - | - |
Day 84 | 0.72 y | 0.82 y | 0.59 y | 0.76 y | 0.78 y | 0.80 y | 0.69 z,u | 0.75 y | 0.063 | - | - | - | - | - | - | - |
Day 105 | 0.93 x | 1.05 x | 0.88 x | 1.04 x | 1.04 x | 1.05 x | 0.98 y | 1.15 x | 0.111 | - | - | - | - | - | - | - |
Day 126 | 1.07 x | 1.26 x | 1.14 x | 1.22 x | 1.03 x | 1.27 x | 1.35 x | 1.27 x | 0.112 | - | - | - | - | - | - | - |
BW, kg | ||||||||||||||||
Overall | 59.5 | 60.6 | 61.6 | 64.1 | 64.4 | 62.2 | 63.0 | 65.1 | 3.33 | 0.01 | 0.04 | 0.33 | 0.23 | 0.3 | 0.1 | 0.41 |
Day 49 | 27.2 w | 28.1 w | 30.9 w | 31.8 w | 32.3 w | 29.6 w | 31.2 v | 30.0 v | 4.52 | - | - | - | - | - | - | - |
Day 63 | 40.2 u | 38.4 u | 44.8 u | 43.0 u | 44.0 u | 40.2 u | 42.7 u | 43.5 u | 4.20 | - | - | - | - | - | - | - |
Day 84 | 55.8 z | 55.5 z | 57.1 z | 58.9 z | 60.2 z | 56.9 z | 57.2 z | 59.1 z | 4.47 | - | - | - | - | - | - | - |
Day 105 | 75.8 y | 77.3 y | 75.7 y | 80.7 y | 82.0 y | 78.8 y | 77.6 y | 83.1 y | 4.18 | - | - | - | - | - | - | - |
Day 126 | 98.7 x | 103.6 x | 99.6 x | 106.2 x | 103.5 x | 105.4 x | 106.0 x | 109.7 x | 4.46 | - | - | - | - | - | - | - |
ADFI, kg/d | ||||||||||||||||
Overall | 2.33 | 2.46 | 2.3 | 2.55 | 2.45 | 2.44 | 2.35 | 2.56 | 0.059 | 0.3 | 0.56 | 0.01 | 0.81 | 0.21 | 0.02 | 0.45 |
Day 49 | 1.24 u | 1.28 v | 1.36 u | 1.36 v | 1.41 u | 1.29 v | 1.35 u | 1.38 u | 0.110 | - | - | - | - | - | - | - |
Day 63 | 1.78 z | 1.99 u | 1.89 z | 2.01 u | 2.09 z | 1.81 u | 1.95 z | 1.99 z | 0.120 | - | - | - | - | - | - | - |
Day 84 | 2.32 y | 2.46 z | 2.31 y | 2.57 z | 2.62 y | 2.42 z | 2.48 y | 2.52 y | 0.220 | - | - | - | - | - | - | - |
Day 105 | 2.96 x | 3.08 y | 2.71 y | 3.15 y | 2.98 x,y | 3.08 y | 2.89 x | 3.33 x | 0.210 | - | - | - | - | - | - | - |
Day 126 | 3.37 x | 3.5 x | 3.24 x | 3.67 x | 3.16 x | 3.58 x | 3.09 x | 3.6 x | 0.218 | - | - | - | - | - | - | - |
G:F | ||||||||||||||||
Overall | 0.38 | 0.38 | 0.38 | 0.37 | 0.36 | 0.38 | 0.4 | 0.38 | 0.032 | 0.676 | 0.391 | 0.669 | 0.244 | 0.635 | 0.237 | 0.447 |
Day 49 | 0.46 x | 0.47 x | 0.46 x | 0.49 x | 0.43 x | 0.47 x | 0.49 x | 0.43 x | 0.030 | - | - | - | - | - | - | - |
Day 63 | 0.50 x | 0.38 x,y | 0.52 x | 0.40 x,y | 0.40 x,y | 0.42 x,y | 0.43 x,y | 0.49 x | 0.070 | - | - | - | - | - | - | - |
Day 84 | 0.31 y | 0.33 y | 0.26 y | 0.29 y | 0.30 y | 0.33 y | 0.28 z | 0.30 y | 0.065 | - | - | - | - | - | - | - |
Day 105 | 0.31 y | 0.34 y | 0.33 y | 0.33 y | 0.35 x,y | 0.34 y | 0.34 y,z | 0.34 x,y | 0.073 | - | - | - | - | - | - | - |
Day 126 | 0.32 y | 0.36 y | 0.35 x,y | 0.33 y | 0.32 y | 0.36 y | 0.49 x | 0.35 x | 0.069 | - | - | - | - | - | - | - |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Share and Cite
Wooten, H.; Kim, H.; Rakhshandeh, A.R.; Rakhshandeh, A. The Effects of a Glucocorticoid Receptor Agonist (GRA) on the Immune Function, Nutrient Digestibility, and Wean-to-Finish Growth Performance of Early-Weaned Pigs. Animals 2020, 10, 953. https://doi.org/10.3390/ani10060953
Wooten H, Kim H, Rakhshandeh AR, Rakhshandeh A. The Effects of a Glucocorticoid Receptor Agonist (GRA) on the Immune Function, Nutrient Digestibility, and Wean-to-Finish Growth Performance of Early-Weaned Pigs. Animals. 2020; 10(6):953. https://doi.org/10.3390/ani10060953
Chicago/Turabian StyleWooten, Hailey, Hwanhee Kim, Amanda R. Rakhshandeh, and Anoosh Rakhshandeh. 2020. "The Effects of a Glucocorticoid Receptor Agonist (GRA) on the Immune Function, Nutrient Digestibility, and Wean-to-Finish Growth Performance of Early-Weaned Pigs" Animals 10, no. 6: 953. https://doi.org/10.3390/ani10060953
APA StyleWooten, H., Kim, H., Rakhshandeh, A. R., & Rakhshandeh, A. (2020). The Effects of a Glucocorticoid Receptor Agonist (GRA) on the Immune Function, Nutrient Digestibility, and Wean-to-Finish Growth Performance of Early-Weaned Pigs. Animals, 10(6), 953. https://doi.org/10.3390/ani10060953