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Article

Encapsulated Bdellovibrio Powder as a Potential Bio-Disinfectant against Whiteleg Shrimp-Pathogenic Vibrios

by
Haipeng Cao
1,*,†,
Huicong Wang
2,†,
Jingjing Yu
1,
Jian An
3 and
Jun Chen
2
1
National Pathogen Collection Center for Aquatic Animals, Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China
2
Department of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong 212400, Jiangsu, China
3
Lianyungang Marine and Fisheries Development Promotion Center, Lianyungang 222000, Jiangsu, China
*
Author to whom correspondence should be addressed.
†
The first two authors contributed equally to this work.
Microorganisms 2019, 7(8), 244; https://doi.org/10.3390/microorganisms7080244
Submission received: 12 May 2019 / Revised: 2 August 2019 / Accepted: 4 August 2019 / Published: 7 August 2019
(This article belongs to the Special Issue Recent Advances in Applied Microbiology)
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Abstract

:
Liquid preparations of bdellovibrios are currently commercialized as water quality improvers to control bacterial pathogens in whiteleg shrimp Penaeus vannamei. However, the efficacy of these liquid preparations is significantly impaired due to a dramatic loss of viable cells during long-term room temperature storage. Thus, new formulations of bdellovibrios are greatly needed for high-stablility room-temperature storage. In the present study, the encapsulated powder of Bdellovibrio sp. strain F16 was prepared using spray drying with 20 g L−1 gelatin as the coating material under a spray flow of 750 L h−1, a feed rate of 12 mL min−1, and an air inlet temperature of 140 °C. It was found to have a cell density of 5.4 × 107 PFU g−1 and to have spherical microparticles with a wrinkled surface and a diameter of 3 μm to 12 μm. In addition, the encapsulated Bdellovibrio powder presented good storage stability with its cell density still remaining at 3.5 × 107 PFU g−1 after 120 days of room-temperature storage; it was safe for freshwater-farmed whiteleg shrimp with an LD50 over 1200 mg L−1, and it exhibited significant antibacterial and protective effects at 0.8 mg L−1 against shrimp-pathogenic vibrios. To our knowledge, this is the first report on a promising Bdellovibrio powder against shrimp vibrios with high stable room-temperature storage.

1. Introduction

The whiteleg shrimp Penaeus vannamei is one of the most important farming shrimp species around the world and is extensively cultivated in Central and South America, USA, East and South East Asia, Middle East, and Africa [1]. However, vibriosis has become a major challenge in shrimp aquaculture because of the lack of effective and safe control agents [2]. For example, infections caused by Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio vulnificus have resulted in significant economic losses in shrimp farming regions [3,4,5]. Thus, new agents to control vibriosis are needed for the sustainable development of the shrimp farming industry.
Predatory bdellovibrios are strongly considered to be an alternative source of antibiotics [6] and have been reported to have the potential to control shrimp pathogens such as Vibrio cholerae [4] and Vibrio parahaemolyticus [7]. Currently, the liquid preparations of bdellovibrios that are used in shrimp aquaculture are commercialized as water quality improvers and are widely available on the market [8]. However, the efficacy of these commercial liquid preparations has been significantly impaired as a result of the massive loss of viable cells during long-term room temperature storage [9]. Hence, effective strategies should be adopted to enhance the stability of viable cells during storage at room temperature.
Spray drying is a most promising encapsulation technique to prolong bacterial survivals in foods or under stress conditions [10,11]. Nowadays, probiotics such as Lactobacillus acidophilus, Lactobacillus rhamnosus, and Bifidobacterium adolescentis have been encapsulated by spray drying to improve the viability of probiotic cells during storage [12,13,14]. The encapsulated bacteriophage powder prepared by spray drying has also been commercialized [15]. However, the encapsulation of bdellovibrios by spray drying has never been reported. In this study, we optimized the production of the encapsulated Bdellovibrio powder by spray drying. The microparticles of the encapsulated Bdellovibrio powder were observed, and room-temperature storage stability, safety, as well as in vitro antibacterial and protective effects against freshwater-cultured whiteleg shrimp-pathogenic vibrios were further evaluated. To our knowledge, this is the first study to develop and characterize a promising Bdellovibrio powder with bactericidal activity against freshwater-cultured whiteleg shrimp-pathogenic vibrios.

2. Materials and Methods

2.1. Microorganisms and Reagents

Bdellovibrio sp. strain F16, previously isolated from the gut of Siberian sturgeon Acipenser baerii and identified by 16S rRNA gene sequencing (deposited in GenBank as accession number HQ225833) [16], was used in this study. Escherichia coli strain DH5α and three freshwater-cultured whiteleg shrimp-pathogenic vibrios (Vibrio cholerae strain BB31, Vibrio parahaemolyticus strain G1, and Vibrio vulnificus strain A2) were obtained from the National Pathogen Collection Center for Aquatic Animals, China. Filtered farm water used in our laboratory was produced by passing 5 m3 of water, which was obtained from Shanghai Yuye Shrimp Farming Co., Ltd., China, successively through 15-denier-size polyester fiber and polyurethane sponge in a water filtration device (Shanghai Haisheng Biotech. Co., Ltd.), with the flow rate of 0.24 m3 min−1 and the circulation rate of 8 times d−1 as recommended by Luo et al. (2008). [17]. Reagents were of analytical grade from the Sinopharm Chemical Reagent Co., Ltd., China.

2.2. Preparation of Bdellovibrio Cells

Prior to the preparation of Bdellovibrio sp. strain F16 cells, E. coli strain DH5α was inoculated into 100 mL of nutrient broth and cultivated under the conditions described by Yu et al. (2010) [18]. Bdellovibrio sp. strain F16 was inoculated into 100 mL of diluted nutrient broth (DNB) [19] containing the prey E. coli, incubated at 30 °C with shaking at 180 rpm for 72 h [20]. The culture filtrate was prepared by a double process of 0.22-μm-pore-size membrane filtration and was carefully examined by transmission electron microscopy [21] and bacteriophage plaque assay [22] to determine that no bacteriophage was present. The cells of Bdellovibrio sp. strain F16 were obtained as described by Cao et al. (2012) [16]. The enumeration of Bdellovibrio sp. strain F16 cells was conducted using the double-layer agar plating method [23] and was recorded as plaque-forming units (PFU) per milliliter.

2.3. Optimization of the Spray Drying Process for Bdellovibrio Cells

Optimization of the spray-drying process for Bdellovibrio sp. strain F16 cells was performed using a single-factor experiment and an orthogonal test as recommended by Wang et al. (2006) [24]. Prior to the spray drying, gelatin was chosen as the coating polymer according to Guergoletto et al. (2017) [25] and was dissolved in distilled water, sterilized at 121 °C for 15 min and maintained at 50 °C according to Gu et al. (2015) [26]. Bdellovibrio sp. strain F16 was assayed for its thermal stability in a water bath at 50 °C for 60 min according to Gao et al. (2016) [27], and enumeration of Bdellovibrio sp. strain F16 was conducted at regular intervals of twenty minutes using the double-layer agar plating method [23] after serial ten-fold dilution [28]. Gelatin concentration, spray flow, feed rate, and air inlet temperature that significantly affect the process for spray drying of probiotic cells [10,29] were selected for further analysis. In the single-factor experiment, three replicates of 200 mL of Bdellovibrio sp. strain F16 (5.0 × 106 PFU mL−1) were incorporated into 800 mL of the coating materials (10, 20, 30, 40, 50 g L−1 of gelatin) at 50 °C and mixed continuously with a magnet mixer at a speed of 100 rpm [29], then the mixtures were, respectively, fed into a laboratory spray dryer (Model SY-6000, Shanghai Shiyuan Bio-engineering Equipment Co. Ltd., China) under conditions of spray flows (650, 700, 750, 800, and 850 L h−1), feed rates (6, 8, 10, 12, and 14 mL min−1), and air inlet temperatures (120, 130, 140, 150, and 160 °C). In the orthogonal test, based on the single-factor analysis, a L9(34) orthogonal design was performed in triplicate to further optimize the spray-drying process. In order to escape from contamination of the spray-dried powder, the compressed air supplied to the spray dryer was filtered using a 0.2 µm-pore-size membrane filter (Source Filter Technology (Hangzhou) Co., Ltd., China) to remove bacteria. The spray-dried samples were collected and stored at a room temperature of 25 °C [30]. The enumeration of Bdellovibrio sp. strain F16 cells in the spray-dried powder was performed using the double-layer agar plating method [23] after serial ten-fold dilution [28] and recorded as PFU per gram.

2.4. Microparticle Observation of Bdellovibrio Powder

The microparticles of Bdellovibrio powder were examined by scanning electron microscopy (S-3400, Hitachi, Japan), as recommended by Ann et al. (2007) [31]. Dry powder was fixed on metal stubs with double-sided tape and coated with gold in a high-vacuum evaporator. Images were taken at a reduced pressure of 9.75 × 10−5 Torr and at an accelerating voltage of 10 kV [29]. In addition, Bdellovibrio sp. strain F16 released from the microparticles was examined by transmission electron microscopy. Briefly, Bdellovibrio powder was dispersed in autoclaved deionized water as described by Song et al. (2014) [32], then the mixture was dripped onto a copper net, negatively stained with 0.5% sodium phosphotungstic acid (pH 7.0) for 1 min as recommended by Falk et al. (1997) [33] and observed under transmission electron microscope (HT7800, Hitachi, Japan). Its plaque forming ability was also examined using the double-layer agar plating method [23], as recommended by Wen et al. (2009) [34].

2.5. Storage Stability of Bdellovibrio PowderAssay

The storage stability of Bdellovibrio powder was performed in triplicate and was checked by detecting the survival of Bdellovibrio sp. strain F16 in the spray-dried powder stored at a room temperature of 25 °C as recommended by Li et al. (2009) [35]. During storage for one hundred and twenty days, the enumeration of Bdellovibrio sp. strain F16 in the spray-dried powder stored at room temperature was conducted at regular intervals of fifteen days using the double-layer agar plating method [23] after serial ten-fold dilution [28]. Another commercial liquid preparation of Bdellovibrio sp. strain F16 (5.4 × 107 PFU mL−1), which was obtained from Shanghai Bio-Green Biotechnology Co. Ltd., China, was stored under the same conditions and served as the control.

2.6. The in Vitro Antibacterial Effect of Bdellovibrio Powder against Shrimp Pathogenic Vibrios Assay

Prior to this assay, suspensions of V. cholerae strain BB31, V. parahaemolyticus strain G1, and V. vulnificus strain A2 were, respectively, prepared as described by Lin et al. (2007) [36]. The gamma-irradiation-killed Bdellovibrio sp. strain F16 was obtained according to Altay et al. (2018) [37], and its powder was prepared using the spray-drying process optimized above. The antibacterial effect of Bdellovibrio powder against the whiteleg shrimp-pathogenic vibrios was conducted in triplicate and carried out in glass flasks. In the treatment flasks, the Bdellovibrio powder and a suspension of a pathogenic strain were independently inoculated into 200 mL of autoclaved filtered farm water to final concentrations of 0.4, 0.8 mg L−1, and 5.0 × 106 CFU mL−1. The mixtures were then incubated at 30 °C with shaking at 180 rpm for 5 days. In the positive control flasks, the gamma-irradiation-killed Bdellovibrio powder (with a final concentration of 0.8 mg L−1) and a suspension of a pathogenic strain (with a final concentration of 5.0 × 106 CFU mL−1) were independently inoculated into autoclaved filtered farm water and incubated as mentioned above. In the negative control flasks, only a pathogenic strain was inoculated in autoclaved filtered farm water and incubated as mentioned above. The cell density of the pathogenic strains was measured at intervals of one day by spread-plate counts on thiosulfate-citrate-bile salts-sucrose (TCBS) agar [38].

2.7. Safety of Bdellovibrio Powder Assay

The safety assay of Bdellovibrio powder was performed according to the Ministry of Agriculture of China (2003) [39] and consisted of one control and six treatment groups. Two hundred and ten healthy freshwater-cultured whiteleg shrimp (averaging 0.55 ± 0.13 g in weight) were obtained from a shrimp farm in Lianyungang, Jiangsu province, China and maintained in a twenty-one glass aquaria (76 cm × 50 cm × 48 cm) supplied with the same aerated filtered farm water with an initial pH of 7.90, 6.5 mg L−1 of dissolved oxygen, 0.12 mg L−1 of total ammonia, and 0.01 mg L−1 of nitrite at 28 °C throughout the experiment. Their health status was assessed through a careful examination of external appearance, gut condition, growth situation, physical behavior, and feeding trends, as recommended by the Marine Products Export Development Authority and the Network of Aquaculture Centers in Asia-Pacific (2003) [40]. Each aquarium, containing 100 L of the same farm water without water recirculation, was stocked with 10 healthy shrimp selected at random. In the treatment groups (three aquaria per group), Bdellovibrio powder from the same batch was independently added into the aerated filtered farm water to final concentrations of 200, 400, 600, 800, 1000, and 1200 mg L−1. Another three aquaria of healthy shrimp, which were exposed to the same experimental conditions, served as the control. The mortality and disease signs were observed and recorded every day for four days in the test shrimp without feeding and water change [29]. The mean lethal dose (LD50) value was calculated according to the graphical probit method, as recommended by Ogbuagu and Iwuchukwu (2014) [41].

2.8. Protective Effect of Bdellovibrio Powder Assay

The protective effect assay of Bdellovibrio powder consisted of three control and three treatment groups. One hundred and eighty healthy freshwater-cultured whiteleg shrimp (averaging 0.62 ± 0.11 g in weight) were obtained from Lianyungang, Jiangsu province, China, and maintained in eighteen glass aquaria (76 cm × 50 cm × 48 cm) supplied with the same aerated filtered farm water with an initial pH of 7.64, 6.6 mg L−1 of dissolved oxygen, 0.10 mg L−1 of total ammonia, and 0.01 mg L−1 of nitrite at 28 °C throughout the experiment. Their health status was assessed through a careful examination, as described above. Each aquarium, containing 100 L of the same farm water without water recirculation, was stocked with 10 healthy shrimp selected at random. In the treatment groups (three aquaria per group), Bdellovibrio powder from the same batch was directly added into the 100 L of aerated filtered farm water to a final concentration of 0.8 mg L−1, as determined above. Immediately thereafter, all of the shrimp were challenged by immersion through continuous exposure to the same batch of freshly cultured shrimp-pathogenic strain (V. cholerae strain BB31, V. parahaemolyticus strain G1, V. vulnificus strain A2) at a final concentration of 5 × 106 CFU mL−1, as recommended by Zhang et al. (2009) [42], Saulnier et al. (2000) [43], and Cao et al. (2015) [4]. In the control groups (three aquaria per group), the shrimp were exposed to the same experimental conditions and only challenged by immersion with the pathogenic strain at the final cell density above. The mortality was observed and recorded every day for six days in the test shrimp without feeding and water change [29]. Any dead shrimp were immediately removed and sampled to re-isolate and confirm specific mortality by the challenge strain. Relative percentage survivals were calculated according to Baulny et al. (1996) [44].

2.9. Statistical Analysis

Statistical analysis was carried out using the statistical software SPSS 15.0 (SPSS, Inc.) to observe the difference in each assay. All of the data were presented as the mean ± standard deviation (SD) for the indicated number of each assay. Differences were considered statistically significant at p < 0.05 using analysis of variance according to Duncan’s test.

2.10. Ethics Statements

The experimental protocol strictly followed the guidelines for ethical review of animal welfare and the general requirements for animal experiment, China. The present experiment was approved by the Institutional Animal Ethics Committee (approval date: 18 Feb. 2016) of Shanghai Ocean University with the permission No. 20171025 dated on 7 Feb. 2017.

3. Results

3.1. Optimization of the Spray-Drying Process for Bdellovibrio Cells

Bdellovibrio sp. strain F16 possessed good thermal stability, as shown in Figure S1, indicating that Bdellovibrio sp. strain F16 could survive at 50 °C. In addition, Bdellovibrio sp. strain F16 cells could be well encapsulated by spray drying with 20 g L−1 of gelatin under spray flows of 650 to 750 L h−1, feed rates of 8–12 mL min−1, and an air inlet temperature of 140 °C (Figure 1). On the basis of the data, the orthogonal test was further designed to optimize the spray-drying process for the Bdellovibrio sp. strain F16 cells. The result showed that the cell densities of Bdellovibrio sp. strain F16 were detected to be 1.45 × 106 to 3.02 × 107 PFU g−1 in the spray-dried powder prepared under different spray-drying conditions (Table 1). The ranking of the four factors in the orthogonal tests was B (spray flow) > A (gelatin concentration) > C (feed rate) > D (air inlet temperature), and the individual levels within each factor were ranked as: A: 2 > 3 > 1; B: 3 > 2 > 1; C: 3 > 1 > 2; D: 2 > 3 > 1. The optimal combination for the spray drying of Bdellovibrio sp. strain F16 was A2B3C3D2 according to the analysis of orthogonal design assistant software version 3.1 (Analytical Software, Internet), indicating that Bdellovibrio powder could be spray dried most effectively with 20 g L−1 gelatin as the coating polymer under the spray flow of 750 L h−1, feed rate of 12 mL min−1, and air inlet temperature of 140 °C. The Bdellovibrio powder prepared under the optimal conditions above was finally demonstrated to have the highest cell density of 5.4 × 107 PFU g−1 and was further investigated in this study.

3.2. Microparticle Morphology of Bdellovibrio Powder

The microparticle morphology of Bdellovibrio powder is shown in Figure 2. The microparticles presented spherical shapes with a wrinkled surface and a diameter of 3 to 12 μm, which is a characteristic feature of spray-dried microparticles containing probiotic cells [25]. Free cells were not visualized under scanning electron microscopy, indicating that all of the Bdellovibrio sp. strain F16 cells were entrapped inside the microparticles. Additionally, the vibroid-shaped Bdellovibrio sp. strain F16 released from the microparticle was observed under transmission electron microscope after the addition of water (Figure 3), which showed the typical morphological features of bdellovibrios [45]. Besides, it could form round plaques as described by Williams et al. (1980) [46] on the double-layer agar plate after incubation for 48 h (Figure S2), which differed from bacteriophage plaques that developed within 24 h [47]. These findings further indicated the presence of bdellovibrios and the absence of bacteriophages in the microparticles.

3.3. Storage Stability of Bdellovibrio Powder

The survival of Bdellovibrio sp. strain F16 in the spray-dried powder and liquid preparation during room temperature storage is shown in Figure 4. The result showed that the survival of Bdellovibrio sp. strain F16 was greatly improved in the encapsulated powder, compared with that in the liquid preparation, with 3.5 × 107 PFU g−1 still alive after 120 days of room-temperature storage. However, a dramatic decline in the cell density of Bdellovibrio sp. strain F16 was observed in the liquid preparation, showing only 4.4 × 103 PFU mL−1 of Bdellovibrio sp. strain F16 alive after 120 days of room-temperature storage. This indicates that the encapsulated Bdellovibrio powder presents better storage stability than the liquid preparation.

3.4. The in Vitro Antibacterial Effect of Bdellovibrio Powder against Shrimp-Pathogenic Vibrios

The in vitro antibacterial effect of Bdellovibrio powder against shrimp-pathogenic vibrios is shown in Figure 5. The result demonstrated that Bdellovibrio powder at 0.8 mg L−1 clearly inhibited the growth of the shrimp-pathogenic vibrios better than Bdellovibrio powder at 0.4 mg L−1 did (p < 0.05). The growth of the pathogenic V. cholerae, V. parahaemolyticus, and V. vulnificus treated with 0.4 and 0.8 mg L−1 of Bdellovibrio powder was significantly inhibited, respectively, showing a reduction of 53.28% (p < 0.05) and 98.80% (p < 0.05), 95.32% (p < 0.05) and 99.97% (p < 0.05), 98.62% (p < 0.05) and 99.91% (p < 0.05) in the cell density after treatment for five days as compared with the negative control. However, a slight increase was observed in the cell densities of the pathogenic V. cholerae, V. parahaemolyticus, and V. vulnificus treated with the gamma-irradiation-killed Bdellovibrio powder after treatment for five days as compared with the negative control. In addition, Bdellovibrio powder at 0.8 mg L−1 had a stronger antibacterial effect against the pathogenic vibrios, and the cell densities of the pathogenic V. cholerae, V. parahaemolyticus, and V. vulnificus were significantly reduced by 58.2% (p < 0.05), 97.6% (p < 0.05), and 89.8% (p < 0.05) after five days of treatment as compared with the initial cell densities. Thus, Bdellovibrio powder was recommended for use at 0.8 mg L−1 to control the pathogenic vibrios.

3.5. Safety of Bdellovibrio Powder

No acute mortality or any visible disease signs were observed in the test whiteleg shrimp treated with 200 to 1200 mg L−1 of Bdellovibrio powder (data not shown). It is concluded that the LD50 value of Bdellovibrio powder is estimated to exceed 1200 mg L−1.

3.6. Protective Effect of Bdellovibrio Powder

The protective effect of Bdellovibrio powder against the challenge of vibriosis in shrimp is shown in Figure 6. The result showed that Bdellovibrio powder at 0.8 mg L−1 could confer significant protection against Vibrio infections in freshwater-farmed P. vannamei. The cumulative mortality of shrimp treated with 0.8 mg L−1 of Bdellovibrio powder was 43.3% (p < 0.05), 70.0% (p < 0.05), and 53.4% (p < 0.05) lower than that in the control after the challenge with the pathogenic V. cholerae, V. parahaemolyticus, and V. vulnificus. The relative percentage survivals of 61.9%, 80.8%, and 69.6% were obtained against the challenge with the V. cholerae, V. parahaemolyticus, and V. vulnificus strains in shrimp for six days. The death of all of the test shrimp was caused by the challenge strains, as determined by bacterial isolation and identification (data not shown).

4. Discussion

Currently, liquid preparations of bdellovibrios have been successfully developed and widely applied in aquaculture [48,49]. However, the spray-dried Bdellovibrio powder has been seldom documented. In the present study, we are the first to report a promising spray-dried Bdellovibrio powder with bactericidal activity against shrimp-pathogenic vibrios.
Various parameters are reported to potentially affect the process for spray drying of probiotic cells [29]. Hence, the optimization of the spray-drying process is critical for the preparation of encapsulated probiotic powder. During spray drying, the coating material, spray flow, feed rate, and air inlet temperature are considered to be the most important factors that influence the encapsulation of probiotic cells [10,29]. Gelatin is known as a safe coating material with no cytotoxicity [50], which has good ability to reduce the heat transfer to the viable cells during spray drying [51]. Therefore, in the present study, gelatin was employed as the coating material, as recommended by Guergoletto et al. (2017) [25]. Furthermore, gelatin concentration, spray flow, feed rate, and air inlet temperature were selected to optimize the spray-drying process by orthogonal design on the basis of the single-factor test, as recommended by Wang et al. (2006) [24]. Using the encapsulated cell density as a key indicator for evaluation of the quality of encapsulated powders [52], the optimized spray-drying process for bdellovibrios was acceptable, as indicated by the high density of encapsulated cells. This makes spray drying an alternative technique for the preparation of encapsulated Bdellovibrio powder.
The spray-dried encapsulated microparticles containing Bifidobacterium and Lactobacillus reuteri cells have been documented to possess characteristic morphological features, i.e., spherical with a wrinkled surface [10,25]. In our study, the encapsulated Bdellovibrio microparticles were also found to be spherical with a wrinkled surface, in accordance with that observed by O’Riordan et al. (2001) [10] and Guergoletto et al. (2017) [25]. This is probably attributed to the inherent characteristics of coating polymers [10], as well as the high temperature used in the drying chamber and the atomized droplets size [53]. In addition, encapsulation of probiotics by spray drying has been confirmed as an effective approach for prolonging cell stability during storage [10]. In our study, a better survival of Bdellovibrio sp. strain F16 was also found in the encapsulated powder than the liquid preparation during room temperature storage. This may be due to the fact that encapsulation can significantly reduce the environmental stress-induced cell death [54].
In order to make its application safe, the candidate probiotic product has to be evaluated for its safety [8,55]. Many studies have reported that bdellovibrios are considered as good probiotics for food and environmental safety [8], which have no cytotoxicity to fish cells [56]; exhibit no hemolytic activity [16]; show no virulence for fish, shrimp, and mice [16,42,57]; and reduce ammonia, nitrite, sulphide, and population of bacterial pathogens in aquaculture water [58,59]. In particular, bdellovibrios are present and abundant only in healthy humans [60], which are positively correlated with gut microbiome diversity [61]. Food with bdellovibrios can act as drivers of gut microbial diversity with no pathogenicity and toxicity to humans [61,62,63], which can restore gut microbiomes and prevent dysbiosis to improve human health [61]. In the present study, the LD50 value of the encapsulated Bdellovibrio powder to whiteleg shrimp exceeded 1200 mg L−1. According to the toxicity rating criteria, as suggested by Yoshimura and Endoh (2005) [64], the encapsulated Bdellovibrio powder can be categorized into a practically nontoxic class (LD50 > 100 mg L−1), indicating that the encapsulated Bdellovibrio powder is a potential safe candidate for use in shrimp aquaculture.
In addition, to consider the encapsulated Bdellovibrio powder as a biodisinfectant against shrimp-pathogenic vibrios, it is essential to obtain data on its antibacterial and protective effects against shrimp-pathogenic vibrios. In our study, the encapsulated Bdellovibrio powder at 0.8 mg L−1 was found to significantly reduce the cell density of the pathogenic V. cholerae, V. parahaemolyticus, and V. vulnificus by 58.2% (p < 0.05), 97.6% (p < 0.05), and 89.8% (p < 0.05) after five days of treatment as compared with the initial cell density. However, the cell density of the pathogenic vibrios did not constantly decline when treated with 0.8 mg L−1 of the Bdellovibrio powder. In view of the fact that gelatin could contribute to the bacterial growth [65], the reduction of the shrimp-pathogenic vibrios is presumably due to live bdellovibrios and gamma-irradiation-susceptible predatory enzymes or other biomolecules. In addition, relative percentage survivals of 61.9%, 80.8%, and 69.6% were also obtained at 0.8 mg L−1 of the encapsulated Bdellovibrio powder against the challenge with the pathogenic V. cholerae, V. parahaemolyticus, and V. vulnificus in shrimp for six days. This is probably due to a primitive immune response in the shrimp induced by bdellovibrios and other immunogenic substances from the powder, which could immediately prime the shrimp to resist bacterial infections. According to the criteria for assessing the effect of probiotic preparations used in aquaculture [39], the encapsulated Bdellovibrio powder can be considered a significant effective biodisinfectant against the shrimp-pathogenic vibrios.
In conclusion, the spray-drying process was optimized in our study through single-factor experiment and orthogonal test to prepare the encapsulated Bdellovibrio powder. The safety, significant antibacterial, and protective effects towards whiteleg shrimp-pathogenic vibrios demonstrated that the encapsulated Bdellovibrio powder could be used as a potential biodisinfectant in freshwater-farmed whiteleg shrimp.

Supplementary Materials

The following are available online at https://www.mdpi.com/2076-2607/7/8/244/s1, Figure S1: Survival of Bdellovibrio sp. strain F16 in a water bath at 50 °C. Figure S2: Plaques of Bdellovibrio sp. strain F16.

Author Contributions

H.C. designed the experimental studies and wrote the manuscript; H.W. and J.Y. conducted the experimental studies; J.A. and J.C. performed the data analysis. All authors read and approved the final manuscript.

Funding

This work has been financially supported by the Fishery Sci-Tech Innovation and Popularization Project of Jiangsu Province (No. Y2017-6 and Y2018-8), the Earmarked Fund for China Modern Shrimp Industry Technology Research (No. CARS-48) and Qingpu District Sci-Tech Development Program, Shanghai China (No. QSD2018-11).

Acknowledgments

The authors thank J.S. Li for providing help with gamma-irradiation of bdellovibrios. Also, we thank the anonymous reviewers and academic editor for their insightful comments on the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

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Microorganisms 07 00244 g001 550
Figure 1. Effects of gelatin concentration (A), spray flow (B), feed rate (C), and air inlet temperature (D) on cell density of Bdellovibrio sp. strain F16 in the spray-dried powder. Data presented as the mean of triplicate spray-drying trials and standard deviations (SD) are indicated by the vertical bars. Bars with different lowercase letters are statistically different (p < 0.05).
Figure 1. Effects of gelatin concentration (A), spray flow (B), feed rate (C), and air inlet temperature (D) on cell density of Bdellovibrio sp. strain F16 in the spray-dried powder. Data presented as the mean of triplicate spray-drying trials and standard deviations (SD) are indicated by the vertical bars. Bars with different lowercase letters are statistically different (p < 0.05).
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Microorganisms 07 00244 g002 550
Figure 2. Microparticles of Bdellovibrio powder. Arrows show the spherical microparticles with a wrinkled surface.
Figure 2. Microparticles of Bdellovibrio powder. Arrows show the spherical microparticles with a wrinkled surface.
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Microorganisms 07 00244 g003 550
Figure 3. Transmission electron microscopy image of Bdellovibrio sp. strain F16 released from the microparticles after the addition of water. White arrow shows the vibroid-shaped Bdellovibrio cell, black arrow shows the amorphous gelatin gel particles around the cell.
Figure 3. Transmission electron microscopy image of Bdellovibrio sp. strain F16 released from the microparticles after the addition of water. White arrow shows the vibroid-shaped Bdellovibrio cell, black arrow shows the amorphous gelatin gel particles around the cell.
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Microorganisms 07 00244 g004 550
Figure 4. Survival of Bdellovibrio sp. strain F16 in the spray-dried powder and liquid preparation during room temperature storage. Data are presented as the mean ± SD.
Figure 4. Survival of Bdellovibrio sp. strain F16 in the spray-dried powder and liquid preparation during room temperature storage. Data are presented as the mean ± SD.
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Microorganisms 07 00244 g005 550
Figure 5. Antibacterial effect of Bdellovibrio powder against the shrimp-pathogenic V. cholerae (A), V. parahaemolyticus (B), V. vulnificus (C); negative control: 0 mg L−1 Bdellovibrio powder; treatment 1: 0.4 mg L−1 Bdellovibrio powder; treatment 2: 0.8 mg L−1 Bdellovibrio powder; positive control: 0.8 mg L−1 gamma-irradiation-killed Bdellovibrio powder. Data are presented as the mean ± SD. Any differences observed are considered statistically significant at p < 0.05 according to Duncan’s test.
Figure 5. Antibacterial effect of Bdellovibrio powder against the shrimp-pathogenic V. cholerae (A), V. parahaemolyticus (B), V. vulnificus (C); negative control: 0 mg L−1 Bdellovibrio powder; treatment 1: 0.4 mg L−1 Bdellovibrio powder; treatment 2: 0.8 mg L−1 Bdellovibrio powder; positive control: 0.8 mg L−1 gamma-irradiation-killed Bdellovibrio powder. Data are presented as the mean ± SD. Any differences observed are considered statistically significant at p < 0.05 according to Duncan’s test.
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Microorganisms 07 00244 g006 550
Figure 6. Protection of whiteleg shrimp by Bdellovibrio powder against the shrimp-pathogenic V. cholerae (A), V. parahaemolyticus (B), and V. vulnificus (C); control: 0 mg L−1 Bdellovibrio powder; treatment: 0.8 mg L−1 Bdellovibrio powder. Data are presented as the mean ± SD. Any differences observed are considered statistically significant at p < 0.05 according to Duncan’s test.
Figure 6. Protection of whiteleg shrimp by Bdellovibrio powder against the shrimp-pathogenic V. cholerae (A), V. parahaemolyticus (B), and V. vulnificus (C); control: 0 mg L−1 Bdellovibrio powder; treatment: 0.8 mg L−1 Bdellovibrio powder. Data are presented as the mean ± SD. Any differences observed are considered statistically significant at p < 0.05 according to Duncan’s test.
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Table
Table 1. L9 (34) orthogonal design to investigate the effect of experimental factors on cell densities of Bdellovibrio sp. strain F16 in the spray-dried powder.
Table 1. L9 (34) orthogonal design to investigate the effect of experimental factors on cell densities of Bdellovibrio sp. strain F16 in the spray-dried powder.
Test No.A (g L−1)B (L h−1)C (mL min−1)D (°C)Cell Density (log PFU g−1)
11565081356.16 ± 0.15 e
215700101406.91 ± 0.13 bcd
315750121457.12 ± 0.09 b
420650101456.74 ± 0.16 d
520700121357.48 ± 0.06 a
62075081407.63 ± 0.01 a
725650121406.77 ± 0.27 cd
82570081456.91 ± 0.02 bcd
925750101357.02 ± 0.11 bc
K16.7306.5576.9006.887
K27.2837.1006.8907.103
K36.9007.2577.1236.923
R0.5530.7000.2330.216
A, gelatin concentration; B, spray flow; C, feed rate; D, air inlet temperature. K1, K2 and K3 are the average scores of level 1, level 2 and level 3 for each factor. R is the range among the average scores for each factor. Data are presented as the mean ± deviations (SD). Values with different superscript letters in the column indicate statistically significant difference (p < 0.05).

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Cao, H.; Wang, H.; Yu, J.; An, J.; Chen, J. Encapsulated Bdellovibrio Powder as a Potential Bio-Disinfectant against Whiteleg Shrimp-Pathogenic Vibrios. Microorganisms 2019, 7, 244. https://doi.org/10.3390/microorganisms7080244

AMA Style

Cao H, Wang H, Yu J, An J, Chen J. Encapsulated Bdellovibrio Powder as a Potential Bio-Disinfectant against Whiteleg Shrimp-Pathogenic Vibrios. Microorganisms. 2019; 7(8):244. https://doi.org/10.3390/microorganisms7080244

Chicago/Turabian Style

Cao, Haipeng, Huicong Wang, Jingjing Yu, Jian An, and Jun Chen. 2019. "Encapsulated Bdellovibrio Powder as a Potential Bio-Disinfectant against Whiteleg Shrimp-Pathogenic Vibrios" Microorganisms 7, no. 8: 244. https://doi.org/10.3390/microorganisms7080244

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