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Exploring the Impact of the Biofloc Rearing System and an Oral WSSV Challenge on the Intestinal Bacteriome of Litopenaeus vannamei

1
Laboratory of Immunology Applied to Aquaculture, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil
2
Laboratory of Marine Shrimp, Department of Aquaculture, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil
3
Ifremer, UMR 5244, IHPE Interactions-Hosts-Pathogens-Environment, UPVD, CNRS, Université de Montpellier, 34095 Montpellier, France
*
Author to whom correspondence should be addressed.
Microorganisms 2018, 6(3), 83; https://doi.org/10.3390/microorganisms6030083
Received: 4 July 2018 / Revised: 3 August 2018 / Accepted: 4 August 2018 / Published: 8 August 2018
(This article belongs to the Special Issue Gut Microorganisms of Aquatic Animals)
We provide a global overview of the intestinal bacteriome of Litopenaeus vannamei in two rearing systems and after an oral challenge by the White spot syndrome virus (WSSV). By using a high-throughput 16S rRNA gene sequencing technology, we identified and compared the composition and abundance of bacterial communities from the midgut of shrimp reared in the super-intensive biofloc technology (BFT) and clear seawater system (CWS). The predominant bacterial group belonged to the phylum Proteobacteria, followed by the phyla Bacteroidetes, Actinobacteria, and Firmicutes. Within Proteobacteria, the family Vibrionaceae, which includes opportunistic shrimp pathogens, was more abundant in CWS than in BFT-reared shrimp. Whereas the families Rhodobacteraceae and Enterobacteriaceae accounted for almost 20% of the bacterial communities of shrimp cultured in BFT, they corresponded to less than 3% in CWS-reared animals. Interestingly, the WSSV challenge dramatically changed the bacterial communities in terms of composition and abundance in comparison to its related unchallenged group. Proteobacteria remained the dominant phylum. Vibrionaceae was the most affected in BFT-reared shrimp (from 11.35 to 20.80%). By contrast, in CWS-reared animals the abundance of this family decreased from 68.23 to 23.38%. Our results provide new evidence on the influence of both abiotic and biotic factors on the gut bacteriome of aquatic species of commercial interest. View Full-Text
Keywords: biofloc technology (BFT); penaeid shrimp; gut microbiota; White spot syndrome virus; 16S rRNA gene sequencing biofloc technology (BFT); penaeid shrimp; gut microbiota; White spot syndrome virus; 16S rRNA gene sequencing
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MDPI and ACS Style

Pilotto, M.R.; Goncalves, A.N.A.; Vieira, F.N.; Seifert, W.Q.; Bachère, E.; Rosa, R.D.; Perazzolo, L.M. Exploring the Impact of the Biofloc Rearing System and an Oral WSSV Challenge on the Intestinal Bacteriome of Litopenaeus vannamei. Microorganisms 2018, 6, 83. https://doi.org/10.3390/microorganisms6030083

AMA Style

Pilotto MR, Goncalves ANA, Vieira FN, Seifert WQ, Bachère E, Rosa RD, Perazzolo LM. Exploring the Impact of the Biofloc Rearing System and an Oral WSSV Challenge on the Intestinal Bacteriome of Litopenaeus vannamei. Microorganisms. 2018; 6(3):83. https://doi.org/10.3390/microorganisms6030083

Chicago/Turabian Style

Pilotto, Mariana R., André N.A. Goncalves, Felipe N. Vieira, Walter Q. Seifert, Evelyne Bachère, Rafael D. Rosa, and Luciane M. Perazzolo 2018. "Exploring the Impact of the Biofloc Rearing System and an Oral WSSV Challenge on the Intestinal Bacteriome of Litopenaeus vannamei" Microorganisms 6, no. 3: 83. https://doi.org/10.3390/microorganisms6030083

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