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Volume 14
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10.3390/microorganisms14040929
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Article

Anti-Hair Loss Activity of Healthy Human Scalp-Derived Staphylococcus capitis KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells

by
Hye-Young Yoo
1,†,
Tae Geun Gil
2,†,
Na-Rin Kim
1,
Hye-Won Lee
1,
Seoyoung Choi
1,
Sung-Jun Choi
3,
Sung-Ha Park
1 and
Byoung-Jun Park
1,*
1
Skin & Natural Products Lab, Kolmar Korea Co., Ltd., Seoul 06800, Republic of Korea
2
Innovative Drug Discovery R&D Institute, BIO-Pharmaceutical Research Center, HK inno.N Co., Ltd., Pangyo 13453, Republic of Korea
3
Division of R&D Strategy, HK inno.N Co., Ltd., Pangyo 13453, Republic of Korea
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Microorganisms 2026, 14(4), 929; https://doi.org/10.3390/microorganisms14040929
Submission received: 26 February 2026 / Revised: 11 April 2026 / Accepted: 17 April 2026 / Published: 20 April 2026
(This article belongs to the Section Medical Microbiology)
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Abstract

Scalp microbes are recognized as contributors to hair loss by influencing scalp homeostasis and hair growth. However, the in vitro anti-hair loss activity of microbial culture media derived from healthy scalps remains unclear. In this study, resident microbes from 20 Korean participants with healthy scalps and hair were isolated, and Staphylococcus capitis was used to produce S. capitis ferment filtrate (SCFF). SCFF anti-hair loss activity was evaluated in human follicle dermal papilla cells (HFDPCs) and human adult low-Calcium High-Temperature (HaCaT) keratinocytes via proliferation assays, qPCR, immunocytochemistry, and SA-β-gal staining at 250–1000 μg/mL. SCFF increased cell density after 48 h in a concentration-dependent manner. In HFDPCs, SCFF controlled growth (KGF, IGF-1, and HGF) and androgen (AR and TGF-β2) factors, regulating key mRNAs for hair growth. SCFF mitigated scalp and hair aging by promoting sirtuins 1 and 7 and collagen type 13, while suppressing p21 and X-Gal staining. In HaCaT cells, SCFF exhibited a scalp barrier-strengthening effect by significantly increasing filaggrin and involucrin levels. It suppressed reactive oxidative stress and exhibited DPPH and ABTS radical scavenging activity. These results suggest that SCFF may modulate key pathways associated with hair loss by promoting scalp and hair anti-aging, barrier strengthening, enhancing antioxidant activity, and supporting hair growth.
Keywords: Staphylococcus capitis; human scalp-derived microbes; anti-hair loss; anti-aging; longevity; scalp barrier; antioxidant Staphylococcus capitis; human scalp-derived microbes; anti-hair loss; anti-aging; longevity; scalp barrier; antioxidant

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MDPI and ACS Style

Yoo, H.-Y.; Gil, T.G.; Kim, N.-R.; Lee, H.-W.; Choi, S.; Choi, S.-J.; Park, S.-H.; Park, B.-J. Anti-Hair Loss Activity of Healthy Human Scalp-Derived Staphylococcus capitis KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells. Microorganisms 2026, 14, 929. https://doi.org/10.3390/microorganisms14040929

AMA Style

Yoo H-Y, Gil TG, Kim N-R, Lee H-W, Choi S, Choi S-J, Park S-H, Park B-J. Anti-Hair Loss Activity of Healthy Human Scalp-Derived Staphylococcus capitis KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells. Microorganisms. 2026; 14(4):929. https://doi.org/10.3390/microorganisms14040929

Chicago/Turabian Style

Yoo, Hye-Young, Tae Geun Gil, Na-Rin Kim, Hye-Won Lee, Seoyoung Choi, Sung-Jun Choi, Sung-Ha Park, and Byoung-Jun Park. 2026. "Anti-Hair Loss Activity of Healthy Human Scalp-Derived Staphylococcus capitis KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells" Microorganisms 14, no. 4: 929. https://doi.org/10.3390/microorganisms14040929

APA Style

Yoo, H.-Y., Gil, T. G., Kim, N.-R., Lee, H.-W., Choi, S., Choi, S.-J., Park, S.-H., & Park, B.-J. (2026). Anti-Hair Loss Activity of Healthy Human Scalp-Derived Staphylococcus capitis KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells. Microorganisms, 14(4), 929. https://doi.org/10.3390/microorganisms14040929

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