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In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress

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Laboratory of Bacterial Pathogenicity, Department of Microbiology, Faculty of Biological Sciences, University of Concepción, Concepción 4070386, Chile
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Laboratorios Pasteur, Concepción 4030000, Chile
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Laboratory of Environmental Microbiology, Department of Microbiology, Faculty of Biological Sciences, University of Concepcion, Concepción 4070386, Chile
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Department of Statistics, Faculty of Physical and Mathematical Sciences, University of Concepción, Concepción 4070386, Chile
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Author to whom correspondence should be addressed.
Pathogens 2020, 9(6), 489; https://doi.org/10.3390/pathogens9060489
Received: 23 April 2020 / Revised: 2 June 2020 / Accepted: 2 June 2020 / Published: 19 June 2020
(This article belongs to the Section Human Pathogens)
Yeasts can adapt to a wide range of pH fluctuations (2 to 10), while Helicobacter pylori, a facultative intracellular bacterium, can adapt to a range from pH 6 to 8. This work analyzed if H. pylori J99 can protect itself from acidic pH by entering into Candida albicans ATCC 90028. Growth curves were determined for H. pylori and C. albicans at pH 3, 4, and 7. Both microorganisms were co-incubated at the same pH values, and the presence of intra-yeast bacteria was evaluated. Intra-yeast bacteria-like bodies were detected using wet mounting, and intra-yeast binding of anti-H. pylori antibodies was detected using immunofluorescence. The presence of the H. pylori rDNA 16S gene in total DNA from yeasts was demonstrated after PCR amplification. H. pylori showed larger death percentages at pH 3 and 4 than at pH 7. On the contrary, the viability of the yeast was not affected by any of the pHs evaluated. H. pylori entered into C. albicans at all the pH values assayed but to a greater extent at unfavorable pH values (pH 3 or 4, p = 0.014 and p = 0.001, respectively). In conclusion, it is possible to suggest that H. pylori can shelter itself within C. albicans under unfavorable pH conditions. View Full-Text
Keywords: Helicobacter pylori; Candida albicans; stress; pH; intracellular bacteria Helicobacter pylori; Candida albicans; stress; pH; intracellular bacteria
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Sánchez-Alonzo, K.; Parra-Sepúlveda, C.; Vega, S.; Bernasconi, H.; Campos, V.L.; Smith, C.T.; Sáez, K.; García-Cancino, A. In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress. Pathogens 2020, 9, 489.

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