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Serologic and Molecular Diagnosis of Anaplasma platys and Ehrlichia canis Infection in Dogs in an Endemic Region

Department of Biomedical Sciences, Ross University School of Veterinary Medicine, Saint Kitts, KN 0101, West Indies
*
Author to whom correspondence should be addressed.
Present Address: College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA.
Pathogens 2020, 9(6), 488; https://doi.org/10.3390/pathogens9060488
Received: 7 May 2020 / Revised: 15 June 2020 / Accepted: 17 June 2020 / Published: 19 June 2020
(This article belongs to the Special Issue Canine and Feline Infectious Diseases)
Anaplasma platys and Ehrlichia canis are obligate intracellular, tick-borne rickettsial pathogens of dogs that may cause life-threatening diseases. In this study, we assessed the usefulness of PCR and a widely used commercial antibody-based point-of-care (POC) test to diagnose A. platys and E. canis infection and updated the prevalence of these pathogens in dogs inhabiting the Caribbean island of Saint Kitts. We detected A. platys in 62/227 (27%), E. canis in 84/227 (37%), and the presence of both in 43/227 (19%) of the dogs using PCR. POC testing was positive for A. platys in 53/187 (28%), E. canis in 112/187 (60%), and for both in 42/187 (22%) of the samples tested. There was only a slight agreement between A. platys PCR and POC test results and a fair agreement for E. canis PCR and POC test results. Our study suggests that PCR testing may be particularly useful in the early stage of infection when antibody levels are low or undetectable, whereas, POC test is useful when false-negative PCR results occur due to low bacteremia. A combination of PCR and POC tests may increase the ability to diagnose A. platys and E. canis infection and consequently will improve patient management. View Full-Text
Keywords: Anaplasma platys; Ehrlichia canis; diagnosis; point-of-care test; PCR Anaplasma platys; Ehrlichia canis; diagnosis; point-of-care test; PCR
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Lara, B.; Conan, A.; Thrall, M.A.; Ketzis, J.K.; Branford, G.C.; Rajeev, S. Serologic and Molecular Diagnosis of Anaplasma platys and Ehrlichia canis Infection in Dogs in an Endemic Region. Pathogens 2020, 9, 488.

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