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Open AccessArticle

Detection of Antibodies against Mycobacterium bovis in Oral Fluid from Eurasian Wild Boar

1
VISAVET Health Surveillance Centre and Animal Health Department, Faculty of Veterinary, Complutense University of Madrid, 28040 Madrid, Spain
2
SaBio. Instituto de Investigación en Recursos Cinegéticos (IREC; CSIC-UCLM), Ronda de Toledo s/n, 13003 Ciudad Real, Spain
*
Author to whom correspondence should be addressed.
These authors have contributed equally to this work.
Pathogens 2020, 9(4), 242; https://doi.org/10.3390/pathogens9040242
Received: 21 February 2020 / Revised: 22 March 2020 / Accepted: 23 March 2020 / Published: 25 March 2020
(This article belongs to the Special Issue Tuberculosis Epidemiology and Control in Multi-Host Systems)
The presence of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex (MTC) is a main concern in wildlife populations such as the Eurasian wild boar (Sus scrofa). Tests detecting antibodies against the MTC are valuable for tuberculosis (TB) monitoring and control and particularly useful in suids. The development of accurate, efficient, and non-invasive new tools to detect exposure to MTC would be highly beneficial for improving disease surveillance. This study aimed to determine if antibodies against MTC could be detected in oral fluid (OF) samples by a new ELISA test (IgG detection) from naturally TB-infected wild boar. For this, individual, paired serum and OF samples were collected from 148 live wild boar in two TB-status areas from Spain and quantitatively used to validate the new ELISA test. Antibodies against MTC were widely detected in OF samples, for which a significant positive correlation (r = 0.83) was found with the validated serology test. OF ELISA sensitivity and specificity were 67.3% and 100%, respectively. The results of this work suggest that OF samples have the potential to be used for MTC diagnosis as a further step in TB surveillance and control in suid populations. Based on our results, further research is warranted and could be performed using non-invasive new tools directly in field conditions to detect exposure to MTC. View Full-Text
Keywords: animal tuberculosis; antibody detection; ELISA test; disease surveillance; oral fluid; Sus scrofa animal tuberculosis; antibody detection; ELISA test; disease surveillance; oral fluid; Sus scrofa
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Barasona, J.A.; Barroso-Arévalo, S.; Rivera, B.; Gortázar, C.; Sánchez-Vizcaíno, J.M. Detection of Antibodies against Mycobacterium bovis in Oral Fluid from Eurasian Wild Boar. Pathogens 2020, 9, 242.

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