Effects of Inactivated Mycobacterium bovis Vaccination on Molokai-Origin Wild Pigs Experimentally Infected with Virulent M. bovis
Abstract
:1. Introduction
2. Results
2.1. Animals
2.2. Gross and Microscopic Lesion Scores
2.3. Culture
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Vaccination
4.3. Challenge
4.4. Necropsy
4.5. Culture
4.6. Statistics
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Tissue (Gross Lesion Scores/Microscopic Lesion Score/M. bovis Culture Outcome: +/-) | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Lung Lobe | Organ | Lymph Node/Lymphoid Tissue | |||||||||||||||||
Treatment Group | Total Gross Lesion Score | Total Microscopic Lesion Score | ID | Caud2,3 | Cran2,4 | Card2,5 | Acc6 | Liver | Spleen | Kidney | Mand2,7 | Parotid2 | Retro-pharyngeal2 | Superf Cerv2,8 | Medias9 | Tracheo-bronch2,10 | Hep11 | Mes12 | Tonsil |
Vaccinates | 18 | 21 | C | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 1,3/2,3/+ | 0/0/- | 1,4/1,3/+ | 0/0/- | 0/0/- | 0/0/+ | 0/0/+ | 0/0/- | 0/0/- |
D | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 4/3/+ | 0/0/+ | 0/0/- | 0/0/- | 0/2/+ | 0/0/+ | 0/0/- | 0/0/- | |||
E | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 1/3/- | 0/1/+ | 0/0/- | 0/0/- | |||
H | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | |||
K | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | |||
L | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | |||
M | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 4/3/+ | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | |||
N | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | |||
Controls | 36 | 33 | A | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- |
B | 0/1/+13 | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 4/3,1/+ | 0/0/- | 0/1/- | 0/0/- | 0/0/- | 0/2/- | 0/0/- | 0/0/- | 0/0/- | |||
F | 0/0/- | 0/0/- | 5/3,3/+ | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/+ | 4,3/3,3/+ | 0/0/- | 0/0/- | 0/0/- | |||
G | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | |||
I | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 4/3/+ | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/2/- | 0/0/- | 0/0/- | 0/0/- | |||
J | 0/0/- | 0/0/- | 5,5/2,3/+ | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 3,3/3/+ | 0/0/- | 0/0/- | 0/0/- | |||
O | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- | 0/0/- |
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Nol, P.; Wehtje, M.E.; Bowen, R.A.; Robbe-Austerman, S.; Thacker, T.C.; Lantz, K.; Rhyan, J.C.; Baeten, L.A.; Juste, R.A.; Sevilla, I.A.; et al. Effects of Inactivated Mycobacterium bovis Vaccination on Molokai-Origin Wild Pigs Experimentally Infected with Virulent M. bovis. Pathogens 2020, 9, 199. https://doi.org/10.3390/pathogens9030199
Nol P, Wehtje ME, Bowen RA, Robbe-Austerman S, Thacker TC, Lantz K, Rhyan JC, Baeten LA, Juste RA, Sevilla IA, et al. Effects of Inactivated Mycobacterium bovis Vaccination on Molokai-Origin Wild Pigs Experimentally Infected with Virulent M. bovis. Pathogens. 2020; 9(3):199. https://doi.org/10.3390/pathogens9030199
Chicago/Turabian StyleNol, Pauline, Morgan E. Wehtje, Richard A. Bowen, Suelee Robbe-Austerman, Tyler C. Thacker, Kristina Lantz, Jack C. Rhyan, Laurie A. Baeten, Ramón A. Juste, Iker A. Sevilla, and et al. 2020. "Effects of Inactivated Mycobacterium bovis Vaccination on Molokai-Origin Wild Pigs Experimentally Infected with Virulent M. bovis" Pathogens 9, no. 3: 199. https://doi.org/10.3390/pathogens9030199