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Open AccessArticle

Serological and Molecular Identification of Brucella spp. in Pigs from Cairo and Giza Governorates, Egypt

Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, 07743 Jena, Germany
Institute for Animal Hygiene and Environmental Health, Free University of Berlin, 14163 Berlin, Germany
Department of Pathobiology, College of Veterinary and Animal Sciences (Sub-Campus UVAS-Lahore), Jhang 35200, Pakistan
Veterinary Service Department, Armed Forces Logistics Authority, Egyptian Armed Forces, 11765 Nasr City, Egypt
Veterinary Serum and Vaccine Research Institute, 11517 Abbasaia, Cairo, Egypt
Faculty of Veterinary Medicine, Kafrelsheikh University, 33516 Kafr El-Sheikh, Egypt
Author to whom correspondence should be addressed.
Pathogens 2019, 8(4), 248;
Received: 23 October 2019 / Revised: 14 November 2019 / Accepted: 16 November 2019 / Published: 20 November 2019
(This article belongs to the Section Animal Pathogens)
Brucellosis is considered as endemic disease of animals and humans since thousands of years in Egypt. However, brucellosis in pigs has never been reported in Egypt. Thus, serological and molecular assays were applied to detect anti-Brucella antibodies and DNA in serum samples collected from pigs. In total 331 blood samples collected from male and female pigs at slaughterhouses of Cairo and Giza governorates were investigated using Brucella c- and i-ELISA and Brucella real-time PCR. Anti-Brucella antibodies were detected in 16 (4.83%) and 36 (10.8%) sera by i-ELISA and c-ELISA, respectively. Brucella DNA was detected in 10 (3.02%) seropositive samples and identified as Brucella melitensis (7/10) and Brucella suis (3/10). A higher prevelance was found in boars. This is the first study investigating pig brucellosis in Egypt. The results of this study will raise awareness for brucellosis in these farm animals and will help to develop effective control strategies. View Full-Text
Keywords: brucellosis; swine; Egypt; ELISA; real-time PCR brucellosis; swine; Egypt; ELISA; real-time PCR
MDPI and ACS Style

Khan, A.U.; Melzer, F.; El-Soally, S.A.G.E.; Elschner, M.C.; Mohamed, S.A.; Sayed Ahmed, M.A.; Roesler, U.; Neubauer, H.; El-Adawy, H. Serological and Molecular Identification of Brucella spp. in Pigs from Cairo and Giza Governorates, Egypt. Pathogens 2019, 8, 248.

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