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Genetic Profiling of Aggregatibacter actinomycetemcomitans Serotype B Isolated from Periodontitis Patients Living in Sweden
Open AccessArticle

Differential Cell Lysis Among Periodontal Strains of JP2 and Non-JP2 Genotype of Aggregatibacter actinomycetemcomitans Serotype B Is Not Reflected in Dissimilar Expression and Production of Leukotoxin

1
Section for Pediatric Dentistry, Department of Dentistry and Oral Health, Health, Aarhus University, 8000 Aarhus, Denmark
2
Department of Clinical Microbiology, Aarhus University Hospital Skejby, 8200 Aarhus, Denmark
3
Section of Molecular Periodontology, Department of Odontology, Faculty of Medicine, Umeå University, 901 87 Umeå, Sweden
4
Division of Oral Microbiology, Department of Odontology, Faculty of Medicine, Umeå University, 901 87 Umeå, Sweden
5
Department of Biomedicine, Aarhus University, 8000 Aarhus, Denmark
*
Author to whom correspondence should be addressed.
Pathogens 2019, 8(4), 211; https://doi.org/10.3390/pathogens8040211
Received: 11 October 2019 / Revised: 25 October 2019 / Accepted: 26 October 2019 / Published: 30 October 2019
Leukotoxic potential of Aggregatibacter actinomycetemcomitans strains has been studied by the use of several methods, and results differ depending on the methods used. The aim of the present study was to perform a comprehensive examination of the leukotoxic potential of a collection of A. actinomycetemcomitans strains by use of three quantitative methods, Western blotting, ELISA, and mRNA expression assay and compare these results with previous data obtained by a cell lysis assay. A higher leukotoxic potential among JP2 genotype strains compared to non-JP2 genotype strains of A. actinomycetemcomitans was found by Western blotting, ELISA and mRNA expression assay. Leukotoxicity as determined by cell lysis assay showed a variation among strains examined, not only depending on being part of JP2 genotype vs. non-JP2 genotype group of A. actinomycetemcomitans. The leukotoxicity of A. actinomycetemcomitans strains as determined by cell lysis assay did not correspond to the leukotoxic potential of A. actinomycetemcomitans strains as determined by three quantitative methods. A comparison of the results obtained by ELISA and mRNA expression assay showed a reasonable correlation between these two methods. It seems important to use more than one method to assess the LtxA-related virulence capacity of A. actinomycetemcomitans in order to obtain comprehensive understanding of the leukotoxic potential of A. actinomycetemcomitans strains. View Full-Text
Keywords: mRNA assay; quantitative ELISA; cell lysis assay; leukotoxin; JP2 genotype mRNA assay; quantitative ELISA; cell lysis assay; leukotoxin; JP2 genotype
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Jensen, A.B.; Lund, M.; Nørskov-Lauritsen, N.; Johansson, A.; Claesson, R.; Reinholdt, J.; Haubek, D. Differential Cell Lysis Among Periodontal Strains of JP2 and Non-JP2 Genotype of Aggregatibacter actinomycetemcomitans Serotype B Is Not Reflected in Dissimilar Expression and Production of Leukotoxin. Pathogens 2019, 8, 211.

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