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Genetic Profiling of Aggregatibacter actinomycetemcomitans Serotype B Isolated from Periodontitis Patients Living in Sweden

1
Division of Molecular Periodontology, Department of Odontology, Umeå University, 907 00 Umeå, Sweden
2
Division of Oral Microbiology, Department of Odontology, Umeå University, 907 00 Umeå, Sweden
3
Section for Pediatric Dentistry, Department of Dentistry and Oral Health, Aarhus University, 8000 Aarhus, Denmark
*
Author to whom correspondence should be addressed.
Pathogens 2019, 8(3), 153; https://doi.org/10.3390/pathogens8030153
Received: 28 August 2019 / Revised: 10 September 2019 / Accepted: 15 September 2019 / Published: 17 September 2019
The bacterium Aggregatibacter actinomycetemcomitans is associated with aggressive forms of periodontitis and with systemic diseases, such as endocarditis. By assessing a Ghanaian longitudinal adolescent cohort, we earlier recognized the cagE gene as a possible diagnostic marker for a subgroup of JP2 and non-JP2 genotype serotype b A. actinomycetemcomitans strains, associated with high leukotoxicity as determined in a semi-quantitative cell assay. This group of A. actinomycetemcomitans is associated with the progression of attachment loss. In the present work, we used conventional polymerase chain reaction (PCR) and quantitative PCR to perform the cagE genotyping of our collection of 116 selected serotype b A. actinomycetemcomitans strains, collected over a period of 15 years from periodontitis patients living in Sweden. The A. actinomycetemcomitans strains carrying cagE (referred to as cagE+; n = 49) were compared to the cagE-negative strains (n = 67), present at larger proportions in the subgingival plaque samples, and were also much more prevalent in the young (≤35 years) compared to in the old (>35 years) group of patients. Our present results underline the potential use of cagE genotyping in the risk assessment of the development of periodontal attachment loss in Swedish adolescents. View Full-Text
Keywords: Aggregatibacter actinomycetemcomitans; cagE; virB1; virB4; genotype; virulence Aggregatibacter actinomycetemcomitans; cagE; virB1; virB4; genotype; virulence
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Johansson, A.; Claesson, R.; Höglund Åberg, C.; Haubek, D.; Lindholm, M.; Jasim, S.; Oscarsson, J. Genetic Profiling of Aggregatibacter actinomycetemcomitans Serotype B Isolated from Periodontitis Patients Living in Sweden. Pathogens 2019, 8, 153.

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