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Volume 8
Issue 4
10.3390/pathogens8040183
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Article

Whole Transcriptome Analyses Reveal Differential mRNA and microRNA Expression Profiles in Primary Human Dermal Fibroblasts Infected with Clinical or Vaccine Strains of Varicella Zoster Virus

by
Soo-Jin Oh
1,
Sooyeon Lim
1,
Moon Jung Song
2,
Jin Hyun Ahn
3,
Chan Hee Lee
4 and
Ok Sarah Shin
1,*
1
Bk21 PLUS Program, Department of Biomedical Sciences, College of Medicine, Korea University Guro Hospital, Seoul 08308, Korea
2
Department of Biosystems and Biotechnology, Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea
3
Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 16419, Korea
4
Department of Microbiology, Chungbuk National University, Cheongju 28644, Korea
*
Author to whom correspondence should be addressed.
Pathogens 2019, 8(4), 183; https://doi.org/10.3390/pathogens8040183
Submission received: 16 September 2019 / Revised: 2 October 2019 / Accepted: 8 October 2019 / Published: 10 October 2019
(This article belongs to the Special Issue Human Herpesviruses: Diversity and Disease)
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Abstract

Licensed live attenuated vaccines have been developed to prevent varicella zoster virus (VZV) infection, which causes chickenpox and shingles. The genomic sequences of both clinical- and vaccine-derived VZV strains have been analyzed previously. To further characterize the molecular signatures and complexity of wildtype (clinical) versus attenuated (vaccine-derived) VZV-mediated host cellular responses, we performed high-throughput next generation sequencing to quantify and compare the expression patterns of mRNAs and microRNAs (miRNAs) in primary human dermal fibroblasts (HDFs) infected with wildtype (YC01 low passage) and attenuated (YC01 high passage, SuduVax, and VarilRix) VZV strains. 3D-multidimensional scaling of the differentially expressed genes demonstrated the distinct grouping of wildtype and attenuated strains. In particular, we observed that HDFs infected with attenuated strains had more differentially expressed genes (DEGs) involved in the retinoic-acid inducible gene–I-like receptor and interferon-mediated signaling pathways compared with wildtype strains. Additionally, miRNA expression patterns were profiled following the infection of HDFs with VZV. Small RNA sequencing identified that several miRNAs were upregulated, including miR-146a-5p, which has been associated with other herpesvirus infections, whereas let-7a-3p was downregulated in both wildtype and attenuated VZV-infected cells. This study identified genes and miRNAs that may be essential in VZV pathogenesis.
Keywords: Varicella zoster virus; SuduVax; RNA-seq; miRNA Varicella zoster virus; SuduVax; RNA-seq; miRNA

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MDPI and ACS Style

Oh, S.-J.; Lim, S.; Song, M.J.; Ahn, J.H.; Lee, C.H.; Shin, O.S. Whole Transcriptome Analyses Reveal Differential mRNA and microRNA Expression Profiles in Primary Human Dermal Fibroblasts Infected with Clinical or Vaccine Strains of Varicella Zoster Virus. Pathogens 2019, 8, 183. https://doi.org/10.3390/pathogens8040183

AMA Style

Oh S-J, Lim S, Song MJ, Ahn JH, Lee CH, Shin OS. Whole Transcriptome Analyses Reveal Differential mRNA and microRNA Expression Profiles in Primary Human Dermal Fibroblasts Infected with Clinical or Vaccine Strains of Varicella Zoster Virus. Pathogens. 2019; 8(4):183. https://doi.org/10.3390/pathogens8040183

Chicago/Turabian Style

Oh, Soo-Jin, Sooyeon Lim, Moon Jung Song, Jin Hyun Ahn, Chan Hee Lee, and Ok Sarah Shin. 2019. "Whole Transcriptome Analyses Reveal Differential mRNA and microRNA Expression Profiles in Primary Human Dermal Fibroblasts Infected with Clinical or Vaccine Strains of Varicella Zoster Virus" Pathogens 8, no. 4: 183. https://doi.org/10.3390/pathogens8040183

APA Style

Oh, S.-J., Lim, S., Song, M. J., Ahn, J. H., Lee, C. H., & Shin, O. S. (2019). Whole Transcriptome Analyses Reveal Differential mRNA and microRNA Expression Profiles in Primary Human Dermal Fibroblasts Infected with Clinical or Vaccine Strains of Varicella Zoster Virus. Pathogens, 8(4), 183. https://doi.org/10.3390/pathogens8040183

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