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Volume 8
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10.3390/pathogens8030145
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Article

Recombinant Newcastle Disease Virus (NDV) Expressing Sigma C Protein of Avian Reovirus (ARV) Protects against Both ARV and NDV in Chickens

by
Deep Prakash Saikia
1,
Kalpana Yadav
1,
Dinesh C. Pathak
1,
Narayan Ramamurthy
1,
Ajai Lawrence D’Silva
1,
Asok Kumar Marriappan
2,
Saravanan Ramakrishnan
3,
Vikram N. Vakharia
4,
Madhan Mohan Chellappa
1,* and
Sohini Dey
1,*
1
Recombinant DNA Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, India
2
Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, India
3
Immunology Section, Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, India
4
Institute of Marine & Environmental Technology, University of Maryland Baltimore County, Baltimore, MD 21202, USA
*
Authors to whom correspondence should be addressed.
Pathogens 2019, 8(3), 145; https://doi.org/10.3390/pathogens8030145
Submission received: 31 July 2019 / Revised: 25 August 2019 / Accepted: 6 September 2019 / Published: 10 September 2019
(This article belongs to the Special Issue Newcastle Disease Vaccines: Current Research and Future Trends)
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Abstract

Newcastle disease (ND) and avian reovirus (ARV) infections are a serious threat to the poultry industry, which causes heavy economic losses. The mesogenic NDV strain R2B is commonly used as a booster vaccine in many Asian countries to control the disease. In this seminal work, a recombinant NDV strain R2B expressing the sigma C (σC) gene of ARV (rNDV-R2B-σC) was generated by reverse genetics, characterized in vitro and tested as a bivalent vaccine candidate in chickens. The recombinant rNDV-R2B-σC virus was attenuated as compared to the parent rNDV-R2B virus as revealed by standard pathogenicity assays. The generated vaccine candidate, rNDV-R2B-σC, could induce both humoral and cell mediated immune responses in birds and gave complete protection against virulent NDV and ARV challenges. Post-challenge virus shedding analysis revealed a drastic reduction in NDV shed, as compared to unvaccinated birds.
Keywords: newcastle disease virus; avian reovirus-σC protein; bivalent vaccine candidate; humoral and cell mediated immune responses; protection efficacy newcastle disease virus; avian reovirus-σC protein; bivalent vaccine candidate; humoral and cell mediated immune responses; protection efficacy

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MDPI and ACS Style

Saikia, D.P.; Yadav, K.; Pathak, D.C.; Ramamurthy, N.; D’Silva, A.L.; Marriappan, A.K.; Ramakrishnan, S.; Vakharia, V.N.; Chellappa, M.M.; Dey, S. Recombinant Newcastle Disease Virus (NDV) Expressing Sigma C Protein of Avian Reovirus (ARV) Protects against Both ARV and NDV in Chickens. Pathogens 2019, 8, 145. https://doi.org/10.3390/pathogens8030145

AMA Style

Saikia DP, Yadav K, Pathak DC, Ramamurthy N, D’Silva AL, Marriappan AK, Ramakrishnan S, Vakharia VN, Chellappa MM, Dey S. Recombinant Newcastle Disease Virus (NDV) Expressing Sigma C Protein of Avian Reovirus (ARV) Protects against Both ARV and NDV in Chickens. Pathogens. 2019; 8(3):145. https://doi.org/10.3390/pathogens8030145

Chicago/Turabian Style

Saikia, Deep Prakash, Kalpana Yadav, Dinesh C. Pathak, Narayan Ramamurthy, Ajai Lawrence D’Silva, Asok Kumar Marriappan, Saravanan Ramakrishnan, Vikram N. Vakharia, Madhan Mohan Chellappa, and Sohini Dey. 2019. "Recombinant Newcastle Disease Virus (NDV) Expressing Sigma C Protein of Avian Reovirus (ARV) Protects against Both ARV and NDV in Chickens" Pathogens 8, no. 3: 145. https://doi.org/10.3390/pathogens8030145

APA Style

Saikia, D. P., Yadav, K., Pathak, D. C., Ramamurthy, N., D’Silva, A. L., Marriappan, A. K., Ramakrishnan, S., Vakharia, V. N., Chellappa, M. M., & Dey, S. (2019). Recombinant Newcastle Disease Virus (NDV) Expressing Sigma C Protein of Avian Reovirus (ARV) Protects against Both ARV and NDV in Chickens. Pathogens, 8(3), 145. https://doi.org/10.3390/pathogens8030145

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