Cellular MicroRNA Expression Profile of Chicken Macrophages Infected with Newcastle Disease Virus Vaccine Strain LaSota
Abstract
:1. Introduction
2. Results and Discussion
2.1. Preliminary Analysis of Small RNA Sequencing
2.2. Differentially Expressed miRNAs in Chicken Macrophages Infected with NDV Vaccine Strain LaSota
2.3. Target Gene Prediction and Functional Annotation Analyses for DE miRNAs after NDV Infection in Chicken Macrophages
3. Material and Methods
3.1. In Vitro Infection of HD11 Cells with LaSota
3.2. RNA Extraction
3.3. Small RNA Library Preparation and Illumina Sequencing
3.4. Quantitative Real-Time RT-PCR (qRT-PCR)
3.5. Sequencing Data Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Number | Id | Sequences (5′–3′) | Regulated | 24 hpi vs. 0 hpi | 48 hpi vs. 0 hpi | ||
---|---|---|---|---|---|---|---|
Log2 FC | p Value | Log2 FC | p Value | ||||
1 | gga-novel-1170-mature | UAUGGGAGGAACUGAAUGACAUG | up | 2.05 | 1.24 × 10−2 | 1.82 | 1.63 × 10−2 |
2 | gga-novel-800-mature | UGGGCGGCUGCGGGAGGG | up | 3.76 | 1.27 × 10−3 | 3.10 | 2.11 × 10−2 |
3 | gga-miR-6606-5p | GAGGAGCGGGAGGAGCGGGA | up | 1.45 | 1.36 × 10−2 | 1.53 | 7.19 × 10−3 |
4 | gga-novel-743-mature | UGGGGGUGCAGGUGGGGGGCU | up | 1.68 | 4.81 × 10−2 | 2.34 | 4.74 × 10−3 |
5 | gga-novel-138-mature | ACGGGACGGGGCGGGACGGCGC | up | 1.36 | 3.31 × 10−2 | 1.20 | 4.87 × 10−2 |
6 | gga-novel-862-mature | GAGCAAGGUACGGGGGGGU | up | 2.32 | 3.27 × 10−4 | 2.09 | 1.00 × 10−3 |
7 | gga-novel-904-mature | AGCAGAGAGAAGGGAUGAGGCU | up | 1.57 | 1.16 × 10−2 | 1.24 | 4.15 × 10−2 |
8 | gga-novel-417-mature | UGCUGGUAGGGGCCGACGACC | up | 2.46 | 9.16 × 10−3 | 2.49 | 1.15 × 10−2 |
9 | gga-novel-951-mature | AUGGAGGCGUGGGUUUUU | up | 3.21 | 9.57 × 10−6 | 3.50 | 3.59 × 10−7 |
10 | gga-novel-200-star | UGGGGAGGCCGCAGUGCAGGGCAA | up | 2.99 | 1.02 × 10−2 | 2.63 | 2.22 × 10−2 |
11 | gga-miR-1584 | CCGGGUGGGGCUGGGCUGGG | up | 2.36 | 9.62 × 10−3 | 3.22 | 1.10 × 10−4 |
12 | gga-novel-672-mature | CCGCGGGGUGGGCGGGGGGCG | up | 2.10 | 7.98 × 10−3 | 2.44 | 6.66 × 10−4 |
13 | gga-novel-880-mature | UGCCGCUGCCCGGUGCUCACACU | down | −3.79 | 2.28 × 10−3 | −2.84 | 1.14 × 10−2 |
14 | gga-novel-226-mature | CGCAGCUCCGUUCCGUCCCCG | down | −1.62 | 2.75 × 10−2 | −1.41 | 4.23 × 10−2 |
15 | gga-novel-91-mature | UCCGCAGCUCCACUCCUGUCAC | down | −1.25 | 4.03 × 10−2 | −2.23 | 3.67 × 10−4 |
16 | gga-novel-20-mature | GCUCCUGCCUGGCUCGCCA | down | −3.64 | 1.71 × 10−8 | −1.31 | 3.19 × 10−2 |
17 | gga-novel-892-mature | UGCCGCUGCCCGGUGCUCACACU | down | −3.79 | 2.28 × 10−3 | −2.84 | 1.14 × 10−2 |
18 | gga-novel-95-mature | CUGCACUGCCACGCCGCGUUCC | down | −1.30 | 2.07 × 10−2 | −1.13 | 4.48 × 10−2 |
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Mu, J.; Liu, X.; Yu, X.; Li, J.; Fei, Y.; Ding, Z.; Yin, R. Cellular MicroRNA Expression Profile of Chicken Macrophages Infected with Newcastle Disease Virus Vaccine Strain LaSota. Pathogens 2019, 8, 123. https://doi.org/10.3390/pathogens8030123
Mu J, Liu X, Yu X, Li J, Fei Y, Ding Z, Yin R. Cellular MicroRNA Expression Profile of Chicken Macrophages Infected with Newcastle Disease Virus Vaccine Strain LaSota. Pathogens. 2019; 8(3):123. https://doi.org/10.3390/pathogens8030123
Chicago/Turabian StyleMu, Jiaqi, Xinxin Liu, Xibing Yu, Junjiao Li, Yidong Fei, Zhuang Ding, and Renfu Yin. 2019. "Cellular MicroRNA Expression Profile of Chicken Macrophages Infected with Newcastle Disease Virus Vaccine Strain LaSota" Pathogens 8, no. 3: 123. https://doi.org/10.3390/pathogens8030123