Genetic and Morphological Identification of Spirometra decipiens in Snakes and Domestic Dog Found in Cuba
Abstract
:1. Introduction
2. Materials and Methods
2.1. Samples
2.2. Histopathological Findings
2.3. DNA Extraction
2.4. PCR Assays
2.5. Amplicons Detection
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Spirometra Species Analyzed in This Study | Species-Specific Primers Gene Cob (5′-3′) | Amplicon Detection Gene Cob (bp) | Species-Specific Primers Gene Cox 1 (5′-3′) | Amplicon Detection Gene Cox 1 (bp) |
---|---|---|---|---|
S. decipiens | ID:24145860 Forward Se/Sd-1800F (AGTTATTTTCGGTTGGTGCTGTAG) Reverse Sd-2317R (TCCTCCCCCCACACGACAAAA) | 540 | ID:24145870 Forward Se/Sd-7955F (ACGTGGTTTGTGGTGGCTCATTTT) Reverse Sd-8567R (TTATTAACTTCCTAACCAACTTGATAC) | 644 |
S. erinaceieuropaei | ID: 6446563 Forward Se/Sd-1800F (AGTTATTTTCGGTTGGTGCTGTAG) Reverse Se2018R (CCACAAACCCAATAACAAACTA) | 239 | ID: 6446566 Forward Se/Sd-7955F (ACGTGGTTTGTGGTGGCTCATTTT) Reverse Se-8356R (ATGATAGGGTATAGGTGACCA) | 401 |
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Morales, A.; Laird-Pérez, R.M.; Capó, V.; Iglesias, E.; Fonte, L.; Plascencia-Hernández, A.; Calderón, E.J.; Eom, K.S.; de Armas, Y.; Pérez-Gómez, H.R. Genetic and Morphological Identification of Spirometra decipiens in Snakes and Domestic Dog Found in Cuba. Pathogens 2022, 11, 1468. https://doi.org/10.3390/pathogens11121468
Morales A, Laird-Pérez RM, Capó V, Iglesias E, Fonte L, Plascencia-Hernández A, Calderón EJ, Eom KS, de Armas Y, Pérez-Gómez HR. Genetic and Morphological Identification of Spirometra decipiens in Snakes and Domestic Dog Found in Cuba. Pathogens. 2022; 11(12):1468. https://doi.org/10.3390/pathogens11121468
Chicago/Turabian StyleMorales, Alexander, Rebeca M. Laird-Pérez, Virginia Capó, Enrique Iglesias, Luis Fonte, Arturo Plascencia-Hernández, Enrique J. Calderón, Keeseon S. Eom, Yaxsier de Armas, and Héctor R. Pérez-Gómez. 2022. "Genetic and Morphological Identification of Spirometra decipiens in Snakes and Domestic Dog Found in Cuba" Pathogens 11, no. 12: 1468. https://doi.org/10.3390/pathogens11121468
APA StyleMorales, A., Laird-Pérez, R. M., Capó, V., Iglesias, E., Fonte, L., Plascencia-Hernández, A., Calderón, E. J., Eom, K. S., de Armas, Y., & Pérez-Gómez, H. R. (2022). Genetic and Morphological Identification of Spirometra decipiens in Snakes and Domestic Dog Found in Cuba. Pathogens, 11(12), 1468. https://doi.org/10.3390/pathogens11121468