Evaluation of Lactiplantibacillus plantarum KAU007 against Low-Pathogenic Avian Influenza Virus (H9N2)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacteria Culture and CFCS Preparation
2.2. Cell Culture, Cytotoxicity, and Antiviral Activity of CFCS of L. plantarum
2.3. Hemagglutination Assay
2.4. Estimation of Cytokines and Antioxidant Activity after CFCS L. plantarum KAU007
2.5. Statistical Analysis
3. Results and Discussion
3.1. To Evaluate the Antiviral Effect of CFCS of L. plantarum against H9N2 in MDCK Cells
3.2. Evaluation of Antiviral Activity of L. plantarum CFCS against H9N2 Using Embryonated Eggs and Hemagglutination Assay
3.3. Effect of L. plantarum CFCS on Proinflammatory Cytokines
3.4. Effect of L. plantarum CFCS on Antioxidant System
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Groups | Treatment | Survival/Total | Survival Percentage |
---|---|---|---|
Con | PBS | 2/2 | 100% |
H9N2 | 107.5 EID50/0.1 mL | 5/9 | 55% |
H9N2 + KAU007—I | CFCS (2.5 mg/mL) | 3/4 | 75% |
H9N2 + KAU007—II | CFCS (5 mg/mL) | 5/6 | 83% |
H9N2 + KAU007—III | CFCS (10 mg/mL) | 8/9 | 88.88% |
Treatment | ||||
---|---|---|---|---|
Hemagglutinating activity | Con | KAU007 CFCS (2.5 mg/mL) | KAU007 CFCS (5 mg/mL) | KAU007 CFCS (10 mg/mL) |
0 | 1:4 | 1:8 | 1:8 |
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Rather, I.A.; Kamli, M.R.; Sabir, J.S.M.; Ali, S. Evaluation of Lactiplantibacillus plantarum KAU007 against Low-Pathogenic Avian Influenza Virus (H9N2). Pathogens 2022, 11, 1246. https://doi.org/10.3390/pathogens11111246
Rather IA, Kamli MR, Sabir JSM, Ali S. Evaluation of Lactiplantibacillus plantarum KAU007 against Low-Pathogenic Avian Influenza Virus (H9N2). Pathogens. 2022; 11(11):1246. https://doi.org/10.3390/pathogens11111246
Chicago/Turabian StyleRather, Irfan A., Majid Rasool Kamli, Jamal S. M. Sabir, and Sajad Ali. 2022. "Evaluation of Lactiplantibacillus plantarum KAU007 against Low-Pathogenic Avian Influenza Virus (H9N2)" Pathogens 11, no. 11: 1246. https://doi.org/10.3390/pathogens11111246