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Article

Staphylococcus ratti sp. nov. Isolated from a Lab Rat

1
Department of Experimental Biology, Division of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic
2
Department of Experimental Biology, Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
3
Reference Laboratory for Staphylococci, National Institute of Public Health, Šrobárova 48, 100 42 Praha, Czech Republic
4
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7 B, 38124 Braunschweig, Germany
5
Department of Public Health, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Jilei Zhang
Pathogens 2022, 11(1), 51; https://doi.org/10.3390/pathogens11010051
Received: 30 November 2021 / Revised: 27 December 2021 / Accepted: 30 December 2021 / Published: 1 January 2022
(This article belongs to the Special Issue Staphylococcus Infections in Humans and Animals)
Staphylococci from the Staphylococcus intermedius-Staphylococcus hyicus species group include numerous animal pathogens and are an important reservoir of virulence and antimicrobial resistance determinants. Due to their pathogenic potential, they are possible causative agents of zoonoses in humans; therefore, it is important to address the properties of these strains. Here we used a polyphasic taxonomic approach to characterize the coagulase-negative staphylococcal strain NRL/St 03/464T, isolated from the nostrils of a healthy laboratory rat during a microbiological screening of laboratory animals. The 16S rRNA sequence, MALDI-TOF mass spectrometry and positive urea hydrolysis and beta-glucuronidase tests clearly distinguished it from closely related Staphylococcus spp. All analyses have consistently shown that the closest relative is Staphylococcus chromogenes; however, values of digital DNA-DNA hybridization <35.3% and an average nucleotide identity <81.4% confirmed that the analyzed strain is a distinct Staphylococcus species. Whole-genome sequencing and expert annotation of the genome revealed the presence of novel variable genetic elements, including two plasmids named pSR9025A and pSR9025B, prophages, genomic islands and a composite transposon that may confer selective advantages to other bacteria and enhance their survival. Based on phenotypic, phylogenetic and genomic data obtained in this study, the strain NRL/St 03/464T (= CCM 9025T = LMG 31873T = DSM 111348T) represents a novel species with the suggested name Staphylococcus ratti sp. nov. View Full-Text
Keywords: laboratory rat; Staphylococcus; Hyicus-Intermedius species group; taxonomy; whole genome sequencing; variable genetic element; genomic island laboratory rat; Staphylococcus; Hyicus-Intermedius species group; taxonomy; whole genome sequencing; variable genetic element; genomic island
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MDPI and ACS Style

Kovařovic, V.; Sedláček, I.; Petráš, P.; Králová, S.; Mašlaňová, I.; Švec, P.; Neumann-Schaal, M.; Botka, T.; Gelbíčová, T.; Staňková, E.; Doškař, J.; Pantůček, R. Staphylococcus ratti sp. nov. Isolated from a Lab Rat. Pathogens 2022, 11, 51. https://doi.org/10.3390/pathogens11010051

AMA Style

Kovařovic V, Sedláček I, Petráš P, Králová S, Mašlaňová I, Švec P, Neumann-Schaal M, Botka T, Gelbíčová T, Staňková E, Doškař J, Pantůček R. Staphylococcus ratti sp. nov. Isolated from a Lab Rat. Pathogens. 2022; 11(1):51. https://doi.org/10.3390/pathogens11010051

Chicago/Turabian Style

Kovařovic, Vojtěch, Ivo Sedláček, Petr Petráš, Stanislava Králová, Ivana Mašlaňová, Pavel Švec, Meina Neumann-Schaal, Tibor Botka, Tereza Gelbíčová, Eva Staňková, Jiří Doškař, and Roman Pantůček. 2022. "Staphylococcus ratti sp. nov. Isolated from a Lab Rat" Pathogens 11, no. 1: 51. https://doi.org/10.3390/pathogens11010051

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