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Fungal Infection and Inflammation in Cystic Fibrosis
Article

Cystic Fibrosis Sputum Impairs the Ability of Neutrophils to Kill Staphylococcus aureus

1
Department of Infectious Diseases, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602, USA
2
Division of Pulmonology, Allergy/Immunology, Cystic Fibrosis and Sleep, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
*
Author to whom correspondence should be addressed.
Senior authors: equal contributions.
Academic Editor: Bryan Heit
Pathogens 2021, 10(6), 703; https://doi.org/10.3390/pathogens10060703
Received: 16 March 2021 / Revised: 26 May 2021 / Accepted: 26 May 2021 / Published: 4 June 2021
(This article belongs to the Special Issue Respiratory Pathogens in Cystic Fibrosis)
Cystic fibrosis (CF) airway disease is characterized by chronic microbial infections and infiltration of inflammatory polymorphonuclear (PMN) granulocytes. Staphylococcus aureus (S. aureus) is a major lung pathogen in CF that persists despite the presence of PMNs and has been associated with CF lung function decline. While PMNs represent the main mechanism of the immune system to kill S. aureus, it remains largely unknown why PMNs fail to eliminate S. aureus in CF. The goal of this study was to observe how the CF airway environment affects S. aureus killing by PMNs. PMNs were isolated from the blood of healthy volunteers and CF patients. Clinical isolates of S. aureus were obtained from the airways of CF patients. The results show that PMNs from healthy volunteers were able to kill all CF isolates and laboratory strains of S. aureus tested in vitro. The extent of killing varied among strains. When PMNs were pretreated with supernatants of CF sputum, S. aureus killing was significantly inhibited suggesting that the CF airway environment compromises PMN antibacterial functions. CF blood PMNs were capable of killing S. aureus. Although bacterial killing was inhibited with CF sputum, PMN binding and phagocytosis of S. aureus was not diminished. The S. aureus-induced respiratory burst and neutrophil extracellular trap release from PMNs also remained uninhibited by CF sputum. In summary, our data demonstrate that the CF airway environment limits killing of S. aureus by PMNs and provides a new in vitro experimental model to study this phenomenon and its mechanism. View Full-Text
Keywords: cystic fibrosis; PMN; Staphylococcus aureus; killing; sputum; respiratory burst; neutrophil extracellular traps cystic fibrosis; PMN; Staphylococcus aureus; killing; sputum; respiratory burst; neutrophil extracellular traps
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MDPI and ACS Style

Fantone, K.; Tucker, S.L.; Miller, A.; Yadav, R.; Bernardy, E.E.; Fricker, R.; Stecenko, A.A.; Goldberg, J.B.; Rada, B. Cystic Fibrosis Sputum Impairs the Ability of Neutrophils to Kill Staphylococcus aureus. Pathogens 2021, 10, 703. https://doi.org/10.3390/pathogens10060703

AMA Style

Fantone K, Tucker SL, Miller A, Yadav R, Bernardy EE, Fricker R, Stecenko AA, Goldberg JB, Rada B. Cystic Fibrosis Sputum Impairs the Ability of Neutrophils to Kill Staphylococcus aureus. Pathogens. 2021; 10(6):703. https://doi.org/10.3390/pathogens10060703

Chicago/Turabian Style

Fantone, Kayla, Samantha L. Tucker, Arthur Miller, Ruchi Yadav, Eryn E. Bernardy, Rachel Fricker, Arlene A. Stecenko, Joanna B. Goldberg, and Balázs Rada. 2021. "Cystic Fibrosis Sputum Impairs the Ability of Neutrophils to Kill Staphylococcus aureus" Pathogens 10, no. 6: 703. https://doi.org/10.3390/pathogens10060703

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