Use of Taxonomic and Trait-Based Approaches to Evaluate the Effect of Bt maize Expressing Cry1Ie Protein on Non-Target Collembola: A Case Study in Northeast China
by
, ,
Bai-Feng Wang
1,†
,
Feng-Ci Wu
1,†,
Jun-Qi Yin
1,
Zhi-Lei Jiang
1,
Xin-Yuan Song
1,* and 
Gadi V. P. Reddy
2
1
Jilin Provincial Key Laboratory of Agricultural Biotechnology, Agro-Biotechnology Research Institute, Jilin Academy of Agriculture Sciences, Changchun 130033, China
2
USDA-ARS-Southern Insect Management Research Unit,141 Experiment Station Rd., P.O. Box, 346 Stoneville, MS 38776, USA
*
Author to whom correspondence should be addressed.
†
These two authors contributed equally to this paper.
Insects 2021, 12(2), 88; https://doi.org/10.3390/insects12020088
Submission received: 5 January 2021
/
Revised: 15 January 2021
/
Accepted: 18 January 2021
/
Published: 21 January 2021
(This article belongs to the Section Insect Ecology, Diversity and Conservation)
Simple Summary
Bt crops have been planted globally since the first commercial Bt maize was cultivated in the United States in 1996. Bt protein from Bt crops can be released to the soil and may potentially affect the non-target soil fauna. Collembola are one of the three most ubiquitous and abundant soil fauna, they have been widely used as indicators of environmental pollution, IE09S034 is a new cry1Ie maize breed independently developed by China, and Northeast China is the most important location for maize production in China. Therefore, this study aimed to clarify whether non-target soil Collembola were influenced by the cultivation of cry1Ie maize in Northeast China. Our results showed that maize variety had no significant effects on collembolan abundance, diversity, and morphological trait, indicating that two years cultivation of cry1Ie maize does not have a bad influence on Collembola in Northeast China.
Abstract
To evaluate the effect of Bt maize expressing Cry1Ie protein on non-target soil Collembola, a two-year field study was conducted in Northeast China. Bt maize line IE09S034 and its near isoline Zong 31 were selected as experimental crops; we investigated the collembolan community using both taxonomic and trait-based approaches, and elucidated the relationship between environmental variables and the collembolan community using redundancy analysis (RDA).The ANOVA results showed that maize variety neither had significant effect on the parameters based on taxonomic approach (abundance, species richness, Shannon–Wiener index, Pielou’s evenness index), nor on the parameters based on trait-based approach (ocelli number, body length, pigmentation level, and furcula development) in either year. The results of RDA also showed that maize variety did not affect collembolan community significantly. These results suggest that two years cultivation of cry1Ie maize does not affect collembolan community in Northeast China.
