Next Article in Journal
Telenomus remus, a Candidate Parasitoid for the Biological Control of Spodoptera frugiperda in Africa, is already Present on the Continent
Previous Article in Journal
Insights into the Maternal Ancestry of Côte d’Ivoire Honeybees Using the Intergenic Region COI-COII
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle

Effects of Different Pretreatments of DNA Extraction from Dried Specimens of Ladybird Beetles (Coleoptera: Coccinellidae)

1
Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Department of Forest Protection, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510640, China
2
Key laboratory of Bio-pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol, Department of Entomology, South China Agricultural University, Guangzhou 510640, China
3
Guangdong Agriculture Industry Business Polytechnic College, Guangzhou 510507, China
*
Author to whom correspondence should be addressed.
Insects 2019, 10(4), 91; https://doi.org/10.3390/insects10040091
Received: 23 December 2018 / Revised: 20 March 2019 / Accepted: 26 March 2019 / Published: 29 March 2019
  |  
PDF [2192 KB, uploaded 29 March 2019]
  |  

Abstract

Obtaining genetic information from museum specimens is a fundamental component of many fields of research, including DNA barcoding, population genetics, conservation genetics, and phylogenetic analysis. However, acquiring genetic information from museum specimens is challenging because of the difficulty in amplifying the target sequences due to DNA damage and degradation. Different pretreatments can significantly impact the purity and concentration of genomic DNA from museum specimens. Here, we assessed four pretreatment methods—use of 0.9% NaCl buffer, phosphate-buffered saline (PBS), Saline Tris-EDTA (STE) buffer, and sterile water—to determine which pretreatment is most suitable for DNA extraction from dried specimens of ladybird beetles. We completed a comprehensive phylogenetic analysis to test whether the sequences obtained from dried specimens enable proper phylogenetic inference. Our results showed that pretreatment can improve the quality of DNA from dried specimens. The pretreatment effects of 0.9% NaCl buffer and STE buffer were better than those of PBS buffer and sterile water. The phylogenetic analyses results showed that museum specimens can be used to generate cogent phylogenetic inferences. We report the optimum pretreatment methods for DNA extraction from dried ladybird beetles specimens as well as provide evidence for accurately determining phylogenetic relationships for museum specimens. View Full-Text
Keywords: pretreatment; DNA extraction; dry specimens; phylogenetic analysis; Coccinellidae pretreatment; DNA extraction; dry specimens; phylogenetic analysis; Coccinellidae
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Huang, W.; Xie, X.; Liang, X.; Wang, X.; Chen, X. Effects of Different Pretreatments of DNA Extraction from Dried Specimens of Ladybird Beetles (Coleoptera: Coccinellidae). Insects 2019, 10, 91.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Insects EISSN 2075-4450 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top