Simultaneous Quantification of Mixed-Acid Triacylglycerol Positional Isomers and Enantiomers in Palm Oil and Lard by Chiral High-Performance Liquid Chromatography Coupled with Mass Spectrometry
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Reagents
2.2. Preparation of Standard Solutions
2.3. Preparation of Sample Solution
2.4. LC/MS Analysis Using MRM (Multiple Reaction Monitoring) Mode
2.5. Calculation of the Concentration and Recovery of Each TAG Isomer in Palm Oil and Lard
3. Results and Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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sn-OPP | sn-PPO | sn-POP | sn-OPO | sn-OOP | sn-POO | |
---|---|---|---|---|---|---|
Slope (a) | 0.0206 | 0.0227 | 0.0299 | 0.0335 | 0.0339 | 0.0344 |
Intercept (b) | −0.0110 | −0.0100 | −0.0112 | −0.0179 | −0.0126 | −0.0156 |
R2 | 0.9958 | 0.9953 | 0.9956 | 0.9977 | 0.9978 | 0.9976 |
TAG | sn-OPP | sn-PPO | sn-POP | sn-OPO | sn-OOP | sn-POO |
---|---|---|---|---|---|---|
Content (wt%) | 2.1 ± 0.04 | 1.8 ± 0.03 | 19.0 ± 0.36 | 1.2 ± 0.01 | 9.0 ± 0.13 | 6.5 ± 0.03 |
Recovey (%) | 95 | 97 | 113 | 97 | 98 | 106 |
RSD(%) of recovery | 7.1 | 8.0 | 2.2 | 19.8 | 25.0 | 4.0 |
TAG | sn-OPP | sn-PPO | sn-POP | sn-OPO | sn-OOP | sn-POO |
---|---|---|---|---|---|---|
Content (wt%) | 1.2 ± 0.01 | 2.6 ± 0.01 | 1.4 ± 0.00 | 12.8 ± 0.15 | 2.6 ± 0.01 | 4.2 ± 0.02 |
Recovey (%) | 95 | 103 | 102 | 120 | 96 | 102 |
RSD(%) of recovery | 6.4 | 4.4 | 2.9 | 15.3 | 3.4 | 5.6 |
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Nagai, T.; Kinoshita, T.; Kasamatsu, E.; Yoshinaga, K.; Mizobe, H.; Yoshida, A.; Itabashi, Y.; Gotoh, N. Simultaneous Quantification of Mixed-Acid Triacylglycerol Positional Isomers and Enantiomers in Palm Oil and Lard by Chiral High-Performance Liquid Chromatography Coupled with Mass Spectrometry. Symmetry 2020, 12, 1385. https://doi.org/10.3390/sym12091385
Nagai T, Kinoshita T, Kasamatsu E, Yoshinaga K, Mizobe H, Yoshida A, Itabashi Y, Gotoh N. Simultaneous Quantification of Mixed-Acid Triacylglycerol Positional Isomers and Enantiomers in Palm Oil and Lard by Chiral High-Performance Liquid Chromatography Coupled with Mass Spectrometry. Symmetry. 2020; 12(9):1385. https://doi.org/10.3390/sym12091385
Chicago/Turabian StyleNagai, Toshiharu, Tetsuaki Kinoshita, Erika Kasamatsu, Kazuaki Yoshinaga, Hoyo Mizobe, Akihiko Yoshida, Yutaka Itabashi, and Naohiro Gotoh. 2020. "Simultaneous Quantification of Mixed-Acid Triacylglycerol Positional Isomers and Enantiomers in Palm Oil and Lard by Chiral High-Performance Liquid Chromatography Coupled with Mass Spectrometry" Symmetry 12, no. 9: 1385. https://doi.org/10.3390/sym12091385