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Genes 2018, 9(12), 621; https://doi.org/10.3390/genes9120621

DNA-Based Super-Resolution Microscopy: DNA-PAINT

1
EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia
2
ARC Center of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, NSW 2052, Australia
3
School of Biotechnology and Biomolecular Science, University of New South Wales, Sydney, NSW 2052, Australia
4
CSIRO Synthetic Biology Future Science Platform, GPO Box 2583, Brisbane, QLD 4001, Australia
*
Author to whom correspondence should be addressed.
Received: 24 October 2018 / Revised: 2 December 2018 / Accepted: 3 December 2018 / Published: 11 December 2018
(This article belongs to the Special Issue DNA Origami and Aptamer Assemblies)
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Abstract

Super-resolution microscopies, such as single molecule localization microscopy (SMLM), allow the visualization of biomolecules at the nanoscale. The requirement to observe molecules multiple times during an acquisition has pushed the field to explore methods that allow the binding of a fluorophore to a target. This binding is then used to build an image via points accumulation for imaging nanoscale topography (PAINT), which relies on the stochastic binding of a fluorescent ligand instead of the stochastic photo-activation of a permanently bound fluorophore. Recently, systems that use DNA to achieve repeated, transient binding for PAINT imaging have become the cutting edge in SMLM. Here, we review the history of PAINT imaging, with a particular focus on the development of DNA-PAINT. We outline the different variations of DNA-PAINT and their applications for imaging of both DNA origamis and cellular proteins via SMLM. Finally, we reflect on the current challenges for DNA-PAINT imaging going forward. View Full-Text
Keywords: DNA origami; DNA PAINT; DNA; super-resolution microscopy; SMLM; fluorescence microscopy DNA origami; DNA PAINT; DNA; super-resolution microscopy; SMLM; fluorescence microscopy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Nieves, D.J.; Gaus, K.; Baker, M.A.B. DNA-Based Super-Resolution Microscopy: DNA-PAINT. Genes 2018, 9, 621.

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