Tracing the Evolutionary Expansion of a Hyperdiverse Antimicrobial Peptide Gene Family in Mytilus spp.: The MyticalinDB Resource
Abstract
1. Introduction
2. Materials and Methods
2.1. Compilation of Mytilus Genomes
2.2. Identification of Novel Myticalins
2.3. Phylogenetic Analysis
2.4. In Silico Gene Expression Analysis
2.5. Three-Dimensional Structure Prediction
3. Results and Discussion
3.1. MyticalinDB: An Updated Collection of Myticalin Sequences
3.2. Molecular Evolution of Myticalins
3.3. Myticalin Sequence Features
3.3.1. A Subfamily
3.3.2. B Subfamily
3.3.3. C Subfamily
3.3.4. D Subfamily
3.3.5. E Subfamily
3.4. Overview of Myticalin Expression Levels
3.5. Considerations About the Structure of Myticalins
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
AMP | Antimicrobial peptide |
PAV | Presence Absence Variation |
PPRs | Pattern Recognition Receptors |
CDS | Coding Sequence |
PAM | peptidylglycine α-amidating monooxygenase |
TPM | Transcripts per Million |
aa | Amino Acid |
References
- Gerdol, M.; Pallavicini, A. Exploring the Immune Resilience of Mediterranean Mussels: Recent Advances and Future Directions. Fish Shellfish Immunol. 2025, 158, 110147. [Google Scholar] [CrossRef] [PubMed]
- Masanja, F.; Yang, K.; Xu, Y.; He, G.; Liu, X.; Xu, X.; Jiang, X.; Luo, X.; Mkuye, R.; Deng, Y.; et al. Bivalves and Microbes: A Mini-Review of Their Relationship and Potential Implications for Human Health in a Rapidly Warming Ocean. Front. Mar. Sci. 2023, 10, 1182438. [Google Scholar] [CrossRef]
- Gerdol, M.; Gomez-Chiarri, M.; Castillo, M.G.; Figueras, A.; Fiorito, G.; Moreira, R.; Novoa, B.; Pallavicini, A.; Ponte, G.; Roumbedakis, K.; et al. Immunity in Molluscs: Recognition and Effector Mechanisms, with a Focus on Bivalvia. In Advances in Comparative Immunology; Cooper, E.L., Ed.; Springer International Publishing: Cham, Switzerland, 2018; pp. 225–341. ISBN 978-3-319-76768-0. [Google Scholar]
- Allam, B.; Raftos, D. Immune Responses to Infectious Diseases in Bivalves. J. Invertebr. Pathol. 2015, 131, 121–136. [Google Scholar] [CrossRef] [PubMed]
- Canesi, L.; Gallo, G.; Gavioli, M.; Pruzzo, C. Bacteria–Hemocyte Interactions and Phagocytosis in Marine Bivalves. Microsc. Res. Tech. 2002, 57, 469–476. [Google Scholar] [CrossRef]
- Burgos-Aceves, M.A.; Faggio, C. An Approach to the Study of the Immunity Functions of Bivalve Haemocytes: Physiology and Molecular Aspects. Fish Shellfish Immunol. 2017, 67, 513–517. [Google Scholar] [CrossRef]
- Mitta, G.; Hubert, F.; Noël, T.; Roch, P. Myticin, a Novel Cysteine-Rich Antimicrobial Peptide Isolated from Haemocytes and Plasma of the Mussel Mytilus galloprovincialis. Eur. J. Biochem. 1999, 265, 71–78. [Google Scholar] [CrossRef] [PubMed]
- Mitta, G.; Vandenbulcke, F.; Hubert, F.; Salzet, M.; Roch, P. Involvement of Mytilins in Mussel Antimicrobial Defense*. J. Biol. Chem. 2000, 275, 12954–12962. [Google Scholar] [CrossRef]
- Charlet, M.; Chernysh, S.; Philippe, H.; Hetru, C.; Hoffmann, J.A.; Bulet, P. Innate Immunity: Isolation of Several Cysteine-Rich Antimicrobial Peptides from the Blood of a Mollusc, Mytilus edulis*. J. Biol. Chem. 1996, 271, 21808–21813. [Google Scholar] [CrossRef]
- Balseiro, P.; Falcó, A.; Romero, A.; Dios, S.; Martínez-López, A.; Figueras, A.; Estepa, A.; Novoa, B. Mytilus galloprovincialis Myticin C: A Chemotactic Molecule with Antiviral Activity and Immunoregulatory Properties. PLoS ONE 2011, 6, e23140. [Google Scholar] [CrossRef]
- Rey-Campos, M.; Moreira, R.; Romero, A.; Medina-Gali, R.M.; Novoa, B.; Gasset, M.; Figueras, A. Transcriptomic Analysis Reveals the Wound Healing Activity of Mussel Myticin C. Biomolecules 2020, 10, 133. [Google Scholar] [CrossRef]
- Rey-Campos, M.; Novoa, B.; Pallavicini, A.; Gerdol, M.; Figueras, A. Comparative Genomics Reveals a Significant Sequence Variability of Myticin Genes in Mytilus galloprovincialis. Biomolecules 2020, 10, 943. [Google Scholar] [CrossRef] [PubMed]
- Tarr, D.E.K. Establishing a Reference Array for the CS-αβ Superfamily of Defensive Peptides. BMC Res. Notes 2016, 9, 490. [Google Scholar] [CrossRef] [PubMed]
- Zhou, L.; Meng, G.; Zhu, L.; Ma, L.; Chen, K. Insect Antimicrobial Peptides as Guardians of Immunity and Beyond: A Review. Int. J. Mol. Sci. 2024, 25, 3835. [Google Scholar] [CrossRef] [PubMed]
- Wu, Q.; Patočka, J.; Kuča, K. Insect Antimicrobial Peptides, a Mini Review. Toxins 2018, 10, 461. [Google Scholar] [CrossRef]
- Tossi, A.; Sandri, L.; Giangaspero, A. Amphipathic, α-Helical Antimicrobial Peptides. Pept. Sci. 2000, 55, 4–30. [Google Scholar] [CrossRef]
- Schmitt, P.; Gueguen, Y.; Desmarais, E.; Bachère, E.; de Lorgeril, J. Molecular Diversity of Antimicrobial Effectors in the Oyster Crassostrea Gigas. BMC Evol. Biol. 2010, 10, 23. [Google Scholar] [CrossRef]
- Seo, J.-K.; Lee, M.J.; Nam, B.-H.; Park, N.G. cgMolluscidin, a Novel Dibasic Residue Repeat Rich Antimicrobial Peptide, Purified from the Gill of the Pacific Oyster, Crassostrea Gigas. Fish Shellfish Immunol. 2013, 35, 480–488. [Google Scholar] [CrossRef]
- Hong, S.-Y.; Kim, D.-G.; Kim, Y.-O.; Park, J.Y.; Seo, J.-K.; Nam, B.-H.; Hong, Y.-K. Purification and cDNA Cloning of the Antimicrobial Peptide apMolluscidin from the Pen Shell, Atrina Pectinata. Fish Shellfish Immunol. 2018, 81, 408–415. [Google Scholar] [CrossRef]
- Leoni, G.; De Poli, A.; Mardirossian, M.; Gambato, S.; Florian, F.; Venier, P.; Wilson, D.; Tossi, A.; Pallavicini, A.; Gerdol, M. Myticalins: A Novel Multigenic Family of Linear, Cationic Antimicrobial Peptides from Marine Mussels (Mytilus spp.). Mar. Drugs 2017, 15, 261. [Google Scholar] [CrossRef]
- Pacor, S.; Benincasa, M.; Musso, M.V.; Krce, L.; Aviani, I.; Pallavicini, A.; Scocchi, M.; Gerdol, M.; Mardirossian, M. The Proline-Rich Myticalins from Mytilus galloprovincialis Display a Membrane-Permeabilizing Antimicrobial Mode of Action. Peptides 2021, 143, 170594. [Google Scholar] [CrossRef]
- Okimura, K.; Matsubara, K.; Suzuki, R.; Ito, H.; Sato, A.; Sugimoto, K. Examination of the Antimicrobial Peptide Myticalin A6 Active Site. Biol. Pharm. Bull. 2021, 44, 515–521. [Google Scholar] [CrossRef] [PubMed]
- Naha, A.; Ramaiah, S. Novel Antimicrobial Peptide SAAP Mutant as a Better Adjuvant to Sulbactam-Based Treatments Against Clinical Strains of XDR Acinetobacter Baumannii. Probiotics Antimicrob. Proteins 2024, 16, 459–473. [Google Scholar] [CrossRef] [PubMed]
- Cannuel, R.; Beninger, P.G.; McCombie, H.; Boudry, P. Gill Development and Its Functional and Evolutionary Implications in the Blue Mussel Mytilus edulis (Bivalvia: Mytilidae). Biol. Bull. 2009, 217, 173–188. [Google Scholar] [CrossRef]
- Dufour, S.C.; Beninger, P.G. A Functional Interpretation of Cilia and Mucocyte Distributions on the Abfrontal Surface of Bivalve Gills. Mar. Biol. 2001, 138, 295–309. [Google Scholar] [CrossRef]
- Travers, M.-A.; Boettcher Miller, K.; Roque, A.; Friedman, C.S. Bacterial Diseases in Marine Bivalves. J. Invertebr. Pathol. 2015, 131, 11–31. [Google Scholar] [CrossRef]
- Dando, P.R.; Southward, A.J.; Southward, E.C.; Bone, Q. Chemoautotrophic Symbionts in the Gills of the Bivalve Mollusc Lucinoma Borealis and the Sediment Chemistry of Its Habitat. Proc. R. Soc. Lond. B Biol. Sci. 1986, 227, 227–247. [Google Scholar] [CrossRef]
- Wentrup, C.; Wendeberg, A.; Schimak, M.; Borowski, C.; Dubilier, N. Forever Competent: Deep-Sea Bivalves Are Colonized by Their Chemosynthetic Symbionts throughout Their Lifetime. Environ. Microbiol. 2014, 16, 3699–3713. [Google Scholar] [CrossRef]
- Gros, O.; Darrasse, A.; Durand, P.; Frenkiel, L.; Mouëza, M. Environmental Transmission of a Sulfur-Oxidizing Bacterial Gill Endosymbiont in the Tropical Lucinid Bivalve Codakia Orbicularis. Appl. Environ. Microbiol. 1996, 62, 2324–2330. [Google Scholar] [CrossRef]
- Allam, B.; Pales Espinosa, E. Bivalve Immunity and Response to Infections: Are We Looking at the Right Place? Fish Shellfish Immunol. 2016, 53, 4–12. [Google Scholar] [CrossRef]
- Pales Espinosa, E.; Koller, A.; Allam, B. Proteomic Characterization of Mucosal Secretions in the Eastern Oyster, Crassostrea Virginica. J. Proteomics 2016, 132, 63–76. [Google Scholar] [CrossRef]
- Saco, A.; Rey-Campos, M.; Novoa, B.; Figueras, A. Transcriptomic Response of Mussel Gills After a Vibrio Splendidus Infection Demonstrates Their Role in the Immune Response. Front. Immunol. 2020, 11, 615580. [Google Scholar] [CrossRef] [PubMed]
- Saco, A.; Suárez, H.; Novoa, B.; Figueras, A. A Genomic and Transcriptomic Analysis of the C-Type Lectin Gene Family Reveals Highly Expanded and Diversified Repertoires in Bivalves. Mar. Drugs 2023, 21, 254. [Google Scholar] [CrossRef] [PubMed]
- Gerdol, M.; Venier, P.; Pallavicini, A. The Genome of the Pacific Oyster Crassostrea Gigas Brings New Insights on the Massive Expansion of the C1q Gene Family in Bivalvia. Dev. Comp. Immunol. 2015, 49, 59–71. [Google Scholar] [CrossRef]
- Gerdol, M.; Fujii, Y.; Hasan, I.; Koike, T.; Shimojo, S.; Spazzali, F.; Yamamoto, K.; Ozeki, Y.; Pallavicini, A.; Fujita, H. The Purplish Bifurcate Mussel Mytilisepta Virgata Gene Expression Atlas Reveals a Remarkable Tissue Functional Specialization. BMC Genomics 2017, 18, 590. [Google Scholar] [CrossRef]
- Ikuta, T.; Tame, A.; Saito, M.; Aoki, Y.; Nagai, Y.; Sugimura, M.; Inoue, K.; Fujikura, K.; Ohishi, K.; Maruyama, T.; et al. Identification of Cells Expressing Two Peptidoglycan Recognition Proteins in the Gill of the Vent Mussel, Bathymodiolus Septemdierum. Fish Shellfish Immunol. 2019, 93, 815–822. [Google Scholar] [CrossRef] [PubMed]
- Gerdol, M.; Moreira, R.; Cruz, F.; Gómez-Garrido, J.; Vlasova, A.; Rosani, U.; Venier, P.; Naranjo-Ortiz, M.A.; Murgarella, M.; Greco, S.; et al. Massive Gene Presence-Absence Variation Shapes an Open Pan-Genome in the Mediterranean Mussel. Genome Biol. 2020, 21, 275. [Google Scholar] [CrossRef]
- Simon, A. Three New Genome Assemblies of Blue Mussel Lineages: North and South European Mytilus edulis and Mediterranean Mytilus galloprovincialis. BioRxiv 2022. [Google Scholar] [CrossRef]
- Han, G.-D.; Ma, D.-D.; Du, L.-N.; Zhao, Z.-J. Chromosomal-Scale Genome Assembly of the Mediterranean Mussel Mytilus galloprovincialis. Sci. Data 2024, 11, 644. [Google Scholar] [CrossRef]
- Murgarella, M.; Puiu, D.; Novoa, B.; Figueras, A.; Posada, D.; Canchaya, C. A First Insight into the Genome of the Filter-Feeder Mussel Mytilus galloprovincialis. PLoS ONE 2016, 11, e0151561. [Google Scholar] [CrossRef]
- Regan, T.; Hori, T.S.; Bean, T.P. A Chromosome-Scale Mytilus edulis Genome Assembly for Aquaculture, Marine Ecology, and Evolution. G3 GenesGenomesGenetics 2024, 14, jkae138. [Google Scholar] [CrossRef]
- Corrochano-Fraile, A.; Davie, A.; Carboni, S.; Bekaert, M. Evidence of Multiple Genome Duplication Events in Mytilus Evolution. BMC Genom. 2022, 23, 340. [Google Scholar] [CrossRef]
- Paggeot, L.X.; DeBiasse, M.B.; Escalona, M.; Fairbairn, C.; Marimuthu, M.P.A.; Nguyen, O.; Sahasrabudhe, R.; Dawson, M.N. Reference Genome for the California Ribbed Mussel, Mytilus Californianus, an Ecosystem Engineer. J. Hered. 2022, 113, 681–688. [Google Scholar] [CrossRef] [PubMed]
- Gallardo-Escárate, C.; Valenzuela-Muñoz, V.; Nuñez-Acuña, G.; Valenzuela-Miranda, D.; Tapia, F.J.; Yévenes, M.; Gajardo, G.; Toro, J.E.; Oyarzún, P.A.; Arriagada, G.; et al. Chromosome-Level Genome Assembly of the Blue Mussel Mytilus Chilensis Reveals Molecular Signatures Facing the Marine Environment. Genes 2023, 14, 876. [Google Scholar] [CrossRef]
- Li, R.; Zhang, W.; Lu, J.; Zhang, Z.; Mu, C.; Song, W.; Migaud, H.; Wang, C.; Bekaert, M. The Whole-Genome Sequencing and Hybrid Assembly of Mytilus Coruscus. Front. Genet. 2020, 11, 440. [Google Scholar] [CrossRef] [PubMed]
- Yang, J.-L.; Feng, D.-D.; Liu, J.; Xu, J.-K.; Chen, K.; Li, Y.-F.; Zhu, Y.-T.; Liang, X.; Lu, Y. Chromosome-Level Genome Assembly of the Hard-Shelled Mussel Mytilus coruscus, a Widely Distributed Species from the Temperate Areas of East Asia. GigaScience 2021, 10, giab024. [Google Scholar] [CrossRef] [PubMed]
- Liao, Z. Laboratory of Marine Biology Protein Engineering, Marine Science and Technical College, Zhejiang Ocean University, Zhoushan, China. 2025; manuscript in preparation. [Google Scholar]
- Reese, M.G.; Eeckmamn, F.H.; Kulp, D.; Haussler, D. Improved Splice Site Detection in Genie. J. Comput. Biol. 1997, 4, 311–323. [Google Scholar] [CrossRef]
- Edgar, R.C. MUSCLE: Multiple Sequence Alignment with High Accuracy and High Throughput. Nucleic Acids Res. 2004, 32, 1792–1797. [Google Scholar] [CrossRef]
- Trifinopoulos, J.; Nguyen, L.-T.; von Haeseler, A.; Minh, B.Q. W-IQ-TREE: A Fast Online Phylogenetic Tool for Maximum Likelihood Analysis. Nucleic Acids Res. 2016, 44, W232–W235. [Google Scholar] [CrossRef]
- Kalyaanamoorthy, S.; Minh, B.Q.; Wong, T.K.F.; von Haeseler, A.; Jermiin, L.S. ModelFinder: Fast Model Selection for Accurate Phylogenetic Estimates. Nat. Methods 2017, 14, 587–589. [Google Scholar] [CrossRef]
- Rambaut, A. FigTree (v.1.4.4). Available online: https://github.com/rambaut/figtree (accessed on 14 May 2025).
- Gerdol, M.; Saco, A.; Riommi, D.; Greco, S.; Kireta, D.; Edomi, P.; Rey-Campos, M.; Novoa, B.; Figueras, A.; Pallavicini, A. The Mytilin Gene Cluster: Shedding Light on the Enigmatic Origin of Mussel Dispensable Genes. Fish Shellfish Immunol. 2025, 161, 110286. [Google Scholar] [CrossRef]
- Gualandi, N.; Fracarossi, D.; Riommi, D.; Sollitto, M.; Greco, S.; Mardirossian, M.; Pacor, S.; Hori, T.; Pallavicini, A.; Gerdol, M. Unveiling the Impact of Gene Presence/Absence Variation in Driving Inter-Individual Sequence Diversity within the CRP-I Gene Family in Mytilus spp. Genes 2023, 14, 787. [Google Scholar] [CrossRef]
- Wagner, G.P.; Kin, K.; Lynch, V.J. Measurement of mRNA Abundance Using RNA-Seq Data: RPKM Measure Is Inconsistent among Samples. Theory Biosci. 2012, 131, 281–285. [Google Scholar] [CrossRef] [PubMed]
- Yanai, I.; Benjamin, H.; Shmoish, M.; Chalifa-Caspi, V.; Shklar, M.; Ophir, R.; Bar-Even, A.; Horn-Saban, S.; Safran, M.; Domany, E.; et al. Genome-Wide Midrange Transcription Profiles Reveal Expression Level Relationships in Human Tissue Specification. Bioinformatics 2005, 21, 650–659. [Google Scholar] [CrossRef]
- Mirdita, M.; Schütze, K.; Moriwaki, Y.; Heo, L.; Ovchinnikov, S.; Steinegger, M. ColabFold: Making Protein Folding Accessible to All. Nat. Methods 2022, 19, 679–682. [Google Scholar] [CrossRef] [PubMed]
- Steinegger, M.; Söding, J. MMseqs2 Enables Sensitive Protein Sequence Searching for the Analysis of Massive Data Sets. Nat. Biotechnol. 2017, 35, 1026–1028. [Google Scholar] [CrossRef] [PubMed]
- Jumper, J.; Evans, R.; Pritzel, A.; Green, T.; Figurnov, M.; Ronneberger, O.; Tunyasuvunakool, K.; Bates, R.; Žídek, A.; Potapenko, A.; et al. Highly Accurate Protein Structure Prediction with AlphaFold. Nature 2021, 596, 583–589. [Google Scholar] [CrossRef]
- Guo, H.-B.; Perminov, A.; Bekele, S.; Kedziora, G.; Farajollahi, S.; Varaljay, V.; Hinkle, K.; Molinero, V.; Meister, K.; Hung, C.; et al. AlphaFold2 Models Indicate That Protein Sequence Determines Both Structure and Dynamics. Sci. Rep. 2022, 12, 10696. [Google Scholar] [CrossRef]
- Pettersen, E.F.; Goddard, T.D.; Huang, C.C.; Meng, E.C.; Couch, G.S.; Croll, T.I.; Morris, J.H.; Ferrin, T.E. UCSF ChimeraX: Structure Visualization for Researchers, Educators, and Developers. Protein Sci. 2021, 30, 70–82. [Google Scholar] [CrossRef]
- Diz, A.P.; Skibinski, D.O.F. Patterns of Admixture and Introgression in a Mosaic Mytilus galloprovincialis and Mytilus edulis Hybrid Zone in SW England. Mol. Ecol. 2024, 33, e17233. [Google Scholar] [CrossRef]
- Riginos, C.; Cunningham, C.W. Invited Review: Local Adaptation and Species Segregation in Two Mussel (Mytilus edulis × Mytilus trossulus) Hybrid Zones. Mol. Ecol. 2005, 14, 381–400. [Google Scholar] [CrossRef]
- Simon, A.; Arbiol, C.; Nielsen, E.E.; Couteau, J.; Sussarellu, R.; Burgeot, T.; Bernard, I.; Coolen, J.W.P.; Lamy, J.-B.; Robert, S.; et al. Replicated Anthropogenic Hybridisations Reveal Parallel Patterns of Admixture in Marine Mussels. Evol. Appl. 2020, 13, 575–599. [Google Scholar] [CrossRef] [PubMed]
- Pool, M.R. Targeting of Proteins for Translocation at the Endoplasmic Reticulum. Int. J. Mol. Sci. 2022, 23, 3773. [Google Scholar] [CrossRef]
- Gerdol, M.; Schmitt, P.; Venier, P.; Rocha, G.; Rosa, R.D.; Destoumieux-Garzón, D. Functional Insights From the Evolutionary Diversification of Big Defensins. Front. Immunol. 2020, 11, 758. [Google Scholar] [CrossRef] [PubMed]
- Shalev, D.E.; Mor, A.; Kustanovich, I. Structural Consequences of Carboxyamidation of Dermaseptin S3. Biochemistry 2002, 41, 7312–7317. [Google Scholar] [CrossRef] [PubMed]
- Dennison, S.R.; Phoenix, D.A. Influence of C-terminal Amidation on the Efficacy of Modelin-5. Biochemistry 2011, 50, 1514–1523. [Google Scholar] [CrossRef] [PubMed]
- Dennison, S.R.; Morton, L.H.G.; Phoenix, D.A. Effect of Amidation on the Antimicrobial Peptide Aurein 2.5 from Australian Southern Bell Frogs. Protein Pept. Lett. 2012, 19, 586–591. [Google Scholar] [CrossRef]
- Rey-Campos, M.; Novoa, B.; Pallavicini, A.; Gerdol, M.; Figueras, A. Comparative Genomics Reveals 13 Different Isoforms of Mytimycins (A-M) in Mytilus galloprovincialis. Int. J. Mol. Sci. 2021, 22, 3235. [Google Scholar] [CrossRef]
- Umumararungu, T.; Gahamanyi, N.; Mukiza, J.; Habarurema, G.; Katandula, J.; Rugamba, A.; Kagisha, V. Proline, a Unique Amino Acid Whose Polymer, Polyproline II Helix, and Its Analogues Are Involved in Many Biological Processes: A Review. Amino Acids 2024, 56, 50. [Google Scholar] [CrossRef]
- Fahmy, L.; Ali, Y.M.; Seilly, D.; McCoy, R.; Owens, R.M.; Pipan, M.; Christie, G.; Grant, A.J. An Attacin Antimicrobial Peptide, Hill_BB_C10074, from Hermetia Illucens with Anti-Pseudomonas Aeruginosa Activity. BMC Microbiol. 2023, 23, 378. [Google Scholar] [CrossRef]
- Gunne, H.; Hellers, M.; Steiner, H. Structure of Preproattacin and Its Processing in Insect Cells Infected with a Recombinant Baculovirus. Eur. J. Biochem. 1990, 187, 699–703. [Google Scholar] [CrossRef]
- Rosa, R.D.; Alonso, P.; Santini, A.; Vergnes, A.; Bachère, E. High Polymorphism in Big Defensin Gene Expression Reveals Presence–Absence Gene Variability (PAV) in the Oyster Crassostrea Gigas. Dev. Comp. Immunol. 2015, 49, 231–238. [Google Scholar] [CrossRef] [PubMed]
- Olivera, B.M. Conus Peptides: Biodiversity-Based Discovery and Exogenomics*. J. Biol. Chem. 2006, 281, 31173–31177. [Google Scholar] [CrossRef] [PubMed]
- Barghi, N.; Concepcion, G.P.; Olivera, B.M.; Lluisma, A.O. Comparison of the Venom Peptides and Their Expression in Closely Related Conus Species: Insights into Adaptive Post-Speciation Evolution of Conus Exogenomes. Genome Biol. Evol. 2015, 7, 1797–1814. [Google Scholar] [CrossRef] [PubMed]
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Kireta, D.; Decarli, P.; Riommi, D.; Gualandi, N.; Greco, S.; Pallavicini, A.; Gerdol, M. Tracing the Evolutionary Expansion of a Hyperdiverse Antimicrobial Peptide Gene Family in Mytilus spp.: The MyticalinDB Resource. Genes 2025, 16, 816. https://doi.org/10.3390/genes16070816
Kireta D, Decarli P, Riommi D, Gualandi N, Greco S, Pallavicini A, Gerdol M. Tracing the Evolutionary Expansion of a Hyperdiverse Antimicrobial Peptide Gene Family in Mytilus spp.: The MyticalinDB Resource. Genes. 2025; 16(7):816. https://doi.org/10.3390/genes16070816
Chicago/Turabian StyleKireta, Dona, Pietro Decarli, Damiano Riommi, Nicolò Gualandi, Samuele Greco, Alberto Pallavicini, and Marco Gerdol. 2025. "Tracing the Evolutionary Expansion of a Hyperdiverse Antimicrobial Peptide Gene Family in Mytilus spp.: The MyticalinDB Resource" Genes 16, no. 7: 816. https://doi.org/10.3390/genes16070816
APA StyleKireta, D., Decarli, P., Riommi, D., Gualandi, N., Greco, S., Pallavicini, A., & Gerdol, M. (2025). Tracing the Evolutionary Expansion of a Hyperdiverse Antimicrobial Peptide Gene Family in Mytilus spp.: The MyticalinDB Resource. Genes, 16(7), 816. https://doi.org/10.3390/genes16070816