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The Complete Genome of Probiotic Lactobacillus sakei Derived from Plateau Yak Feces

1
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
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Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
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Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
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The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Midlothian EH25 9RG, UK
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Department of Medicine Cholistan, University of Veterinary & Animal Sciences, Bahawalpur 64100, Pakistan
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Department of Pathology, Faculty of Veterinary & Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan
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Laboratory of Detection and Monitoring of Highland Animal Disease, Tibet Agriculture and Animal Husbandry College, Linzhi 860000, China
*
Author to whom correspondence should be addressed.
Genes 2020, 11(12), 1527; https://doi.org/10.3390/genes11121527
Received: 9 November 2020 / Revised: 13 December 2020 / Accepted: 18 December 2020 / Published: 21 December 2020
(This article belongs to the Section Microbial Genetics and Genomics)
Probiotic bacteria are receiving increased attention due to the potential benefits to their hosts. Plateau yaks have resistance against diseases and stress, which is potentially related to their inner probiotics. To uncover the potential functional genes of yak probiotics, we sequenced the whole genome of Lactobacillus sakei (L. sakei). The results showed that the genome length of L. sakei was 1.99 Mbp, with 1943 protein coding genes (21 rRNA, 65 tRNA, and 1 tmRNA). There were three plasmids found in this bacteria, with 88 protein coding genes. EggNOG annotation uncovered that the L. sakei genes were found to belong to J (translation, ribosomal structure, and biogenesis), L (replication, recombination, and repair), G (carbohydrate transport and metabolism), and K (transcription). GO annotation showed that most of the L. sakei genes were related to cellular processes, metabolic processes, biological regulation, localization, response to stimulus, and organization or biogenesis of cellular components. CAZy annotation found that there were 123 CAZys in the L. sakei genome, with glycosyl transferases and glycoside hydrolases. Our results revealed the genome characteristics of L. sakei, which may give insight into the future employment of this probiotic bacterium for its functional benefits. View Full-Text
Keywords: probiotic; yak; Lactobacillus sakei; genome; sequencing probiotic; yak; Lactobacillus sakei; genome; sequencing
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MDPI and ACS Style

Li, K.; Liu, J.; Zeng, Z.; Kulyar, M.F.-e.-A.; Wang, Y.; Li, A.; Bhutta, Z.A.; Aqib, A.I.; Shahzad, M.; Li, J.; Qi, D. The Complete Genome of Probiotic Lactobacillus sakei Derived from Plateau Yak Feces. Genes 2020, 11, 1527. https://doi.org/10.3390/genes11121527

AMA Style

Li K, Liu J, Zeng Z, Kulyar MF-e-A, Wang Y, Li A, Bhutta ZA, Aqib AI, Shahzad M, Li J, Qi D. The Complete Genome of Probiotic Lactobacillus sakei Derived from Plateau Yak Feces. Genes. 2020; 11(12):1527. https://doi.org/10.3390/genes11121527

Chicago/Turabian Style

Li, Kun, Juanjuan Liu, Zhibo Zeng, Muhammad F.-e.-A. Kulyar, Yaping Wang, Aoyun Li, Zeeshan A. Bhutta, Amjad I. Aqib, Muhammad Shahzad, Jiakui Li, and Desheng Qi. 2020. "The Complete Genome of Probiotic Lactobacillus sakei Derived from Plateau Yak Feces" Genes 11, no. 12: 1527. https://doi.org/10.3390/genes11121527

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