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Open AccessArticle

Cdc42 Couples T Cell Receptor Endocytosis to GRAF1-Mediated Tubular Invaginations of the Plasma Membrane

1
Biotechnology Institute Thurgau (BITg) at the University of Konstanz, 8280 Kreuzlingen, Switzerland
2
Department of Biology, University of Konstanz, 78457 Konstanz, Germany
3
EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney 2052, Australia
*
Author to whom correspondence should be addressed.
Cells 2019, 8(11), 1388; https://doi.org/10.3390/cells8111388 (registering DOI)
Received: 16 October 2019 / Revised: 31 October 2019 / Accepted: 31 October 2019 / Published: 4 November 2019
(This article belongs to the Special Issue Membrane Traffic in Health and Disease)
: T cell activation is immediately followed by internalization of the T cell receptor (TCR). TCR endocytosis is required for T cell activation, but the mechanisms supporting removal of TCR from the cell surface remain incompletely understood. Here we report that TCR endocytosis is linked to the clathrin-independent carrier (CLIC) and GPI-enriched endocytic compartments (GEEC) endocytic pathway. We show that unlike the canonical clathrin cargo transferrin or the adaptor protein Lat, internalized TCR accumulates in tubules shaped by the small GTPase Cdc42 and the Bin/amphiphysin/Rvs (BAR) domain containing protein GRAF1 in T cells. Preventing GRAF1-positive tubules to mature into endocytic vesicles by expressing a constitutively active Cdc42 impairs the endocytosis of TCR, while having no consequence on the uptake of transferrin. Together, our data reveal a link between TCR internalization and the CLIC/GEEC endocytic route supported by Cdc42 and GRAF1.
Keywords: T cell receptor; clathrin-independent endocytosis; CLIC; Cdc42; GRAF1 T cell receptor; clathrin-independent endocytosis; CLIC; Cdc42; GRAF1
MDPI and ACS Style

Rossatti, P.; Ziegler, L.; Schregle, R.; Betzler, V.M.; Ecker, M.; Rossy, J. Cdc42 Couples T Cell Receptor Endocytosis to GRAF1-Mediated Tubular Invaginations of the Plasma Membrane. Cells 2019, 8, 1388.

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    Doi: 10.5281/zenodo.3524892
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