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Subcutaneous and Visceral Adipose-Derived Mesenchymal Stem Cells: Commonality and Diversity
Open AccessFeature PaperArticle

Isolation, Characterization, Differentiation and Immunomodulatory Capacity of Mesenchymal Stromal/Stem Cells from Human Perirenal Adipose Tissue

1
Division of Nephrology, Department of Internal Medicine III, University Hospital, Goethe University, 60596 Frankfurt/M., Germany
2
Division of Allergology, Pneumology and Cystic Fibrosis, Department for Children and Adolescents, University Hospital, Goethe University, 60596 Frankfurt/M., Germany
3
Institute of Medical Virology, University Hospital, Goethe University, 60596 Frankfurt/M., Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this paper.
Cells 2019, 8(11), 1346; https://doi.org/10.3390/cells8111346
Received: 12 October 2019 / Revised: 24 October 2019 / Accepted: 29 October 2019 / Published: 29 October 2019
(This article belongs to the Special Issue Adipose-Derived Stromal/Stem Cells)
Mesenchymal stromal/stem cells (MSCs) are immature multipotent cells, which represent a rare population in the perivascular niche within nearly all tissues. The most abundant source to isolate MSCs is adipose tissue. Currently, perirenal adipose tissue is rarely described as the source of MSCs. MSCs were isolated from perirenal adipose tissue (prASCs) from patients undergoing tumor nephrectomies, cultured and characterized by flow cytometry and their differentiation potential into adipocytes, chondrocytes, osteoblasts and epithelial cells. Furthermore, prASCs were stimulated with lipopolysaccharide (LPS), lipoteichoic acid (LTA) or a mixture of cytokines (cytomix). In addition, prASC susceptibility to human cytomegalovirus (HCMV) was investigated. The expression of inflammatory readouts was estimated by qPCR and immunoassay. HCMV infection was analyzed by qPCR and immunostaining. Characterization of cultured prASCs shows the cells meet the criteria of MSCs and prASCs can undergo trilineage differentiation. Cultured prASCs can be induced to differentiate into epithelial cells, shown by cytokeratin 18 expression. Stimulation of prASCs with LPS or cytomix suggests the cells are capable of initiating an inflammation-like response upon stimulation with LPS or cytokines, whereas, LTA did not induce a significant effect on the readouts (ICAM-1, IL-6, TNFα, MCP-1 mRNA and IL-6 protein). HCMV broadly infects prASCs, showing a viral load dependent cytopathological effect (CPE). Our current study summarizes the isolation and culture of prASCs, clearly characterizes the cells, and demonstrates their immunomodulatory potential and high permissiveness for HCMV.
Keywords: mesenchymal stromal/stem cells; perirenal; adipose tissue; fat; characterization; stimulation; lipopolysaccharide; cytokines; cytomegalovirus mesenchymal stromal/stem cells; perirenal; adipose tissue; fat; characterization; stimulation; lipopolysaccharide; cytokines; cytomegalovirus
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MDPI and ACS Style

Baer, P.C.; Koch, B.; Hickmann, E.; Schubert, R.; Cinatl, J., Jr.; Hauser, I.A.; Geiger, H. Isolation, Characterization, Differentiation and Immunomodulatory Capacity of Mesenchymal Stromal/Stem Cells from Human Perirenal Adipose Tissue. Cells 2019, 8, 1346.

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