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Cells 2019, 8(1), 78; https://doi.org/10.3390/cells8010078

Crohn’s Disease Patients in Remission Display an Enhanced Intestinal IgM+ B Cell Count in Concert with a Strong Activation of the Intestinal Complement System

1
Institute of Nutritional Medicine, Molecular Gastroenterology, University Hospital Schleswig-Holstein, Campus Lübeck, D-23538 Lübeck, Germany
2
Department of Medicine B, Gastroenterology and Hepatology, University Hospital Münster, 48149 Münster, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this project.
Received: 20 December 2018 / Revised: 15 January 2019 / Accepted: 17 January 2019 / Published: 21 January 2019
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Abstract

Inflammatory bowel disease (IBD) is an umbrella term that comprises Crohn’s disease (CD) and ulcerative colitis (UC). Both entities are characterized by a disturbed mucosal immune response and an imbalance of intestinal microbiota composition. The complement system (C) plays a critical role in the detection, and clearance of bacteria and dysregulation of single complement components has been linked to IBD. Here, we asked if the C contributes to distinct subtypes of inflammation observed in CD and UC. We performed systematical expression analyses of the intestinal C in IBD patients and controls. Immunohistochemistry or immunoblot experiments were performed to verify qPCR data. Activity of the three activation pathways of C was studied in sera samples. In CD patients a strong upregulation of the C was observed enabling the definition of unique expression patterns being associated either with remission or active disease. These data were reflected by an enhanced C activation in sera and fecal samples. An excessive mucosal presence of immunoglobulin M (IgM) and CR2/CD21 positive B cells in concert with decreased fecal IgA level was identified in CD patients in remission. These findings point to an exacerbated induction of the intestinal C that may potentially be involved in the etiology of CD. View Full-Text
Keywords: intestinal complement system; inflammatory bowel disease; Crohn’s disease; ulcerative colitis; complement receptor 2 (CR2); immunoglobulin M (IgM); B cells intestinal complement system; inflammatory bowel disease; Crohn’s disease; ulcerative colitis; complement receptor 2 (CR2); immunoglobulin M (IgM); B cells
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Preisker, S.; Brethack, A.-K.; Bokemeyer, A.; Bettenworth, D.; Sina, C.; Derer, S. Crohn’s Disease Patients in Remission Display an Enhanced Intestinal IgM+ B Cell Count in Concert with a Strong Activation of the Intestinal Complement System. Cells 2019, 8, 78.

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