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Article

Partial Disassembly of the Nuclear Pore Complex Proteins during Semi-Closed Mitosis in Dictyostelium discoideum

Department of Cell Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam-Golm, Germany
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Academic Editors: Symeon Siniossoglou and Wolfram Antonin
Cells 2022, 11(3), 407; https://doi.org/10.3390/cells11030407
Received: 31 December 2021 / Revised: 19 January 2022 / Accepted: 21 January 2022 / Published: 25 January 2022
(This article belongs to the Special Issue Lipid and Protein Dynamics at the Nuclear Envelope)
Dictyostelium cells undergo a semi-closed mitosis, during which the nuclear envelope (NE) persists; however, free diffusion between the cytoplasm and the nucleus takes place. To permit the formation of the mitotic spindle, the nuclear envelope must be permeabilized in order to allow diffusion of tubulin dimers and spindle assembly factors into the nucleus. In Aspergillus, free diffusion of proteins between the cytoplasm and the nucleus is achieved by a partial disassembly of the nuclear pore complexes (NPCs) prior to spindle assembly. In order to determine whether this is also the case in Dictyostelium, we analysed components of the NPC by immunofluorescence microscopy and live cell imaging and studied their behaviour during interphase and mitosis. We observed that the NPCs are absent from the contact area of the nucleoli and that some nucleoporins also localize to the centrosome and the spindle poles. In addition, we could show that, during mitosis, the central FG protein NUP62, two inner ring components and Gle1 depart from the NPCs, while all other tested NUPs remained at the NE. This leads to the conclusion that indeed a partial disassembly of the NPCs takes place, which contributes to permeabilisation of the NE during semi-closed mitosis. View Full-Text
Keywords: nuclear pore complex; nucleoporins; semi-closed mitosis; centrosome; Dictyostelium nuclear pore complex; nucleoporins; semi-closed mitosis; centrosome; Dictyostelium
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MDPI and ACS Style

Mitic, K.; Grafe, M.; Batsios, P.; Meyer, I. Partial Disassembly of the Nuclear Pore Complex Proteins during Semi-Closed Mitosis in Dictyostelium discoideum. Cells 2022, 11, 407. https://doi.org/10.3390/cells11030407

AMA Style

Mitic K, Grafe M, Batsios P, Meyer I. Partial Disassembly of the Nuclear Pore Complex Proteins during Semi-Closed Mitosis in Dictyostelium discoideum. Cells. 2022; 11(3):407. https://doi.org/10.3390/cells11030407

Chicago/Turabian Style

Mitic, Kristina, Marianne Grafe, Petros Batsios, and Irene Meyer. 2022. "Partial Disassembly of the Nuclear Pore Complex Proteins during Semi-Closed Mitosis in Dictyostelium discoideum" Cells 11, no. 3: 407. https://doi.org/10.3390/cells11030407

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