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Article

YY2 in Mouse Preimplantation Embryos and in Embryonic Stem Cells

1
Regenerative Medicine Program, Instituto Aragonés de Ciencias de la Salud, CIBA, Avenida San Juan Bosco 13, 50009 Zaragoza, Spain
2
Departamento de Anatomía, Embriología y Genética Animal, Facultad de Veterinaria, Universidad de Zaragoza, C/Miguel Servet 177, 50013 Zaragoza, Spain
3
Placental Pathophysiology and Fetal Programming Group, Fundación IISA, Avenida San Juan Bosco 13, 50009 Zaragoza, Spain
4
Department of Neonatology, Charité—Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany
5
Fundación “Agencia Aragonesa para la Investigación y el Desarrollo” (ARAID), 50018 Zaragoza, Spain
*
Author to whom correspondence should be addressed.
Current address: Center for Cooperative Research in Biomaterials (CIC biomaGUNE), 20014 Donostia San Sebastián, Spain.
Current address: CerTest Biotec SL, 50840 Zaragoza, Spain.
Academic Editor: Giorgio Malpeli
Cells 2021, 10(5), 1123; https://doi.org/10.3390/cells10051123
Received: 19 March 2021 / Revised: 22 April 2021 / Accepted: 29 April 2021 / Published: 7 May 2021
Yin Yang 2 encodes a mammalian-specific transcription factor (YY2) that shares high homology in the zinc finger region with both YY1 and REX1/ZFP42, encoded by the Yin Yang 1 and Reduced Expression Protein 1/Zinc Finger Protein 42 gene, respectively. In contrast to the well-established roles of the latter two in gene regulation, X chromosome inactivation and binding to specific transposable elements (TEs), much less is known about YY2, and its presence during mouse preimplantation development has not been described. As it has been reported that mouse embryonic stem cells (mESC) cannot be propagated in the absence of Yy2, the mechanistic understanding of how Yy2 contributes to mESC maintenance remains only very partially characterized. We describe Yy2 expression studies using RT-PCR and staining with a high-affinity polyclonal serum in mouse embryos and mESC. Although YY2 is expressed during preimplantation development, its presence appears dispensable for developmental progress in vitro until formation of the blastocyst. Attenuation of Yy2 levels failed to alter either Zscan4 levels in two-cell embryos or IAP and MERVL levels at later preimplantation stages. In contrast to previous claims that constitutively expressed shRNA against Yy2 in mESC prohibited the propagation of mESC in culture, we obtained colonies generated from mESC with attenuated Yy2 levels. Concomitant with a decreased number of undifferentiated colonies, Yy2-depleted mESC expressed higher levels of Zscan4 but no differences in the expression of TEs or other pluripotency markers including Sox2, Oct4, Nanog and Esrrb were observed. These results confirm the contribution of Yy2 to the maintenance of mouse embryonic stem cells and show the preimplantation expression of YY2. These functions are discussed in relation to mammalian-specific functions of YY1 and REX1. View Full-Text
Keywords: Yin Yang 2; YY2; preimplantation development; transposable elements; MERVL; mouse embryonic stem cells; self-renewal; Zscan4; expanded potency Yin Yang 2; YY2; preimplantation development; transposable elements; MERVL; mouse embryonic stem cells; self-renewal; Zscan4; expanded potency
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MDPI and ACS Style

Pérez-Palacios, R.; Climent, M.; Santiago-Arcos, J.; Macías-Redondo, S.; Klar, M.; Muniesa, P.; Schoorlemmer, J. YY2 in Mouse Preimplantation Embryos and in Embryonic Stem Cells. Cells 2021, 10, 1123. https://doi.org/10.3390/cells10051123

AMA Style

Pérez-Palacios R, Climent M, Santiago-Arcos J, Macías-Redondo S, Klar M, Muniesa P, Schoorlemmer J. YY2 in Mouse Preimplantation Embryos and in Embryonic Stem Cells. Cells. 2021; 10(5):1123. https://doi.org/10.3390/cells10051123

Chicago/Turabian Style

Pérez-Palacios, Raquel, María Climent, Javier Santiago-Arcos, Sofía Macías-Redondo, Martin Klar, Pedro Muniesa, and Jon Schoorlemmer. 2021. "YY2 in Mouse Preimplantation Embryos and in Embryonic Stem Cells" Cells 10, no. 5: 1123. https://doi.org/10.3390/cells10051123

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