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Article

Production of Virus-Free Garlic Plants through Somatic Embryogenesis

1
Department of Plant Breeding, Genetics and Biometrics, University of Zagreb Faculty of Agriculture, Svetošimunska Cesta 25, 10 000 Zagreb, Croatia
2
Department of Experimental Plant Biology, Charles University Faculty of Science, Viničná 5, 128 44 Prague, Czech Republic
3
Institute of Agriculture and Tourism, Karla Huguesa 8, 52440 Poreč, Croatia
4
Centre of Excellence for Biodiversity and Molecular Plant Breeding (CroP-BioDiv), Svetošimunska Cesta 25, 10 000 Zagreb, Croatia
5
Department of Plant Pathology, University of Zagreb Faculty of Agriculture, Svetošimunska Cesta 25, 10 000 Zagreb, Croatia
6
Department of Plant Nutrition, University of Zagreb Faculty of Agriculture, Svetošimunska Cesta 25, 10 000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Academic Editor: Giovanni Iapichino
Agronomy 2021, 11(5), 876; https://doi.org/10.3390/agronomy11050876
Received: 30 March 2021 / Revised: 19 April 2021 / Accepted: 27 April 2021 / Published: 29 April 2021
(This article belongs to the Special Issue Role of Plant Tissue Culture in Agricultural Research and Production)
The present study was conducted to establish a protocol for the regeneration of virus-free garlic plants through somatic embryogenesis of two Croatian garlic ecotypes. Basal parts of cloves from mother plants were cultured on a full Murashige and Skoog (MS) or modified MS medium (¼ of KNO3 and NH4NO3 and 2xMgSO4) containing 0.1 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D) or 1 mg L−1 2,4-D + 0.5 mg L−1 kinetin (Kin) and representing four different treatments. Plants were regenerated in MS medium containing 0.1 mg L−1 2,4-D and rooted in a medium containing 0.05 mg L−1 1-naphthaleneacetic acid (NAA) + 0.005 mg L−1 6-(γ,γ-dimethylallylamino)purine (2iP). The presence of viruses (i.e., sanitary status) of the mother plants and regenerants was checked by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). The mother plants were infected with onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYSV). In addition, the presence of garlic common latent virus (GCLV) was confirmed in four mother plants. Embryogenic callus developed in all four treatments with success ranging from 55% to 81% depending on treatment and ecotype. Plant conversion was significantly higher in somatic embryos developed in media containing 0.1 mg L−1 2,4-D than those developed in media containing 1 mg L−1 2,4-D + 0.5 mg L−1 Kin. Virus elimination success ranged from 13.3% up to 62.5% depending on garlic ecotype and treatment. The overall rate of virus elimination by somatic embryogenesis for both treatments and ecotypes were 20.7%, 22.9%, and 30.5% for OYDV, GCLV, and LYSV, respectively. Based on these results, somatic embryogenesis has been shown to be equally or more successful in eliminating garlic viruses compared to other in vitro methods. View Full-Text
Keywords: Allium sativum; callogenesis; somatic embryos; virus elimination Allium sativum; callogenesis; somatic embryos; virus elimination
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MDPI and ACS Style

Kereša, S.; Kurtović, K.; Ban, S.G.; Vončina, D.; Jerčić, I.H.; Bolarić, S.; Lazarević, B.; Godena, S.; Ban, D.; Mihovilović, A.B. Production of Virus-Free Garlic Plants through Somatic Embryogenesis. Agronomy 2021, 11, 876. https://doi.org/10.3390/agronomy11050876

AMA Style

Kereša S, Kurtović K, Ban SG, Vončina D, Jerčić IH, Bolarić S, Lazarević B, Godena S, Ban D, Mihovilović AB. Production of Virus-Free Garlic Plants through Somatic Embryogenesis. Agronomy. 2021; 11(5):876. https://doi.org/10.3390/agronomy11050876

Chicago/Turabian Style

Kereša, Snježana, Katarina Kurtović, Smiljana G. Ban, Darko Vončina, Ivanka H. Jerčić, Snježana Bolarić, Boris Lazarević, Sara Godena, Dean Ban, and Anita B. Mihovilović. 2021. "Production of Virus-Free Garlic Plants through Somatic Embryogenesis" Agronomy 11, no. 5: 876. https://doi.org/10.3390/agronomy11050876

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