Next Article in Journal
Evaluation of Humic Fertilizers Applied at Full and Reduced Nitrogen Rates on Kentucky Bluegrass Quality and Soil Health
Next Article in Special Issue
Production of Virus-Free Garlic Plants through Somatic Embryogenesis
Previous Article in Journal
Can Hydromulch Reduce the Emergence of Perennial Weeds?
Previous Article in Special Issue
Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application
Article

Suspension Cell Culture of Dioscorea deltoidea—A Renewable Source of Biomass and Furostanol Glycosides for Food and Pharmaceutical Industry

1
K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276 Moscow, Russia
2
Biology Faculty, M.V. Lomonosov Moscow State University, 119234 Moscow, Russia
3
All-Russian State Research Institute for Control Standardization and Certification of Veterinary Preparations, 123022 Moscow, Russia
4
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 109316 Moscow, Russia
5
Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju 361-763, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Paul Michael Hasegawa
Agronomy 2021, 11(2), 394; https://doi.org/10.3390/agronomy11020394
Received: 19 January 2021 / Revised: 13 February 2021 / Accepted: 18 February 2021 / Published: 23 February 2021
(This article belongs to the Special Issue Role of Plant Tissue Culture in Agricultural Research and Production)
Dioscorea deltoidea is a medicinal plant valued for its high content of steroidal glycosides (SG)—bioactive compounds with cardioprotective and immunomodulation actions, also used to treat reproductive system disorders. To overcome the limitations of natural resources of this species, a suspension cell culture of D. deltoidea was developed as a renewable and ecologically sustainable source of raw biomass and SG. Cell culture demonstrated stable and intensive growth in the laboratory (20 L) and industrial (630 L) bioreactors operated under a semi-continuous regime (specific growth rate 0.11–1.12 day−1, growth index 3.5–3.7). Maximum dry weight accumulation (8.5–8.8 g/L) and SG content (47–57 mg/g DW) were recorded during the stationary phase. Bioreactor-produced cell biomass contained inorganic macro (K, Ca, Mg, Na) and micro (Zn, Mn, Fe, B, Al, Cu, Cr, Se, Co, Ni) elements in concentrations within the safe range of dietary recommendations. Acute toxicity test showed no or insignificant changes in organ weight, hematological panel and blood biochemistry of laboratory animals fed with 2000 and 5000 mg/kg dry biomass. The results suggest that cell culture of D. deltoidea grown in bioreactors has great potential to be used as functional foods and a component of specialized dietary supplements in complex therapy of reproductive system disorders and mineral deficiency. View Full-Text
Keywords: bioreactors; cellular agriculture; mineral composition; plant cell culture; protodioscin; steroidal glycosides bioreactors; cellular agriculture; mineral composition; plant cell culture; protodioscin; steroidal glycosides
Show Figures

Figure 1

MDPI and ACS Style

Titova, M.V.; Popova, E.V.; Konstantinova, S.V.; Kochkin, D.V.; Ivanov, I.M.; Klyushin, A.G.; Titova, E.G.; Nebera, E.A.; Vasilevskaya, E.R.; Tolmacheva, G.S.; Kotenkova, E.A.; Nosov, A.M.; Paek, K.-Y. Suspension Cell Culture of Dioscorea deltoidea—A Renewable Source of Biomass and Furostanol Glycosides for Food and Pharmaceutical Industry. Agronomy 2021, 11, 394. https://doi.org/10.3390/agronomy11020394

AMA Style

Titova MV, Popova EV, Konstantinova SV, Kochkin DV, Ivanov IM, Klyushin AG, Titova EG, Nebera EA, Vasilevskaya ER, Tolmacheva GS, Kotenkova EA, Nosov AM, Paek K-Y. Suspension Cell Culture of Dioscorea deltoidea—A Renewable Source of Biomass and Furostanol Glycosides for Food and Pharmaceutical Industry. Agronomy. 2021; 11(2):394. https://doi.org/10.3390/agronomy11020394

Chicago/Turabian Style

Titova, Maria V., Elena V. Popova, Svetlana V. Konstantinova, Dmitry V. Kochkin, Igor M. Ivanov, Andrey G. Klyushin, Elena G. Titova, Elena A. Nebera, Ekaterina R. Vasilevskaya, Galina S. Tolmacheva, Elena A. Kotenkova, Alexandr M. Nosov, and Kee-Yoeup Paek. 2021. "Suspension Cell Culture of Dioscorea deltoidea—A Renewable Source of Biomass and Furostanol Glycosides for Food and Pharmaceutical Industry" Agronomy 11, no. 2: 394. https://doi.org/10.3390/agronomy11020394

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop