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Article

Structural Characterization and Antioxidant Potential of Chitosan by γ-Irradiation from the Carapace of Horseshoe Crab

1
Horseshoe Crab Research Unit, Department of Bioscience & Biotechnology, Fakir Mohan University, Balasore 756089, Odisha, India
2
Institute of Tropical Biodiversity and Sustainable Development, University Malaysia Terengganu, Kuala Nerus 21030, Terengganu, Malaysia
3
Research Divisions, Association for Biodiversity Conservation and Research, Devine Colony, Balasore 756001, Odisha, India
4
Aquamarina Research Foundation, Dona Paula, Panaji 403004, Goa, India
5
Centre of Excellence (CoE) for Bioresource Management and Energy Conservation Material Development, Fakir Mohan University, Balasore 756089, Odisha, India
6
Department of Food Technology and Biochemical Engineering, Faculty of Engineering and Technology, Jadavpur University, Jadavpur, Kolkata 700032, West Bengal, India
7
Malda Polytechnic, West Bengal State Council of Technical Education, Govt. of West Bengal, Malda 732102, West Bengal, India
8
School of Marine and Environmental Sciences, University Malaysia Terengganu, Kuala Nerus 21030, Terengganu, Malaysia
9
Forensic Science Programme, School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian 16150, Kelantan, Malaysia
10
School of Biotechnology, GIET University, Gunupur 765022, Odisha, India
*
Authors to whom correspondence should be addressed.
Polymers 2020, 12(10), 2361; https://doi.org/10.3390/polym12102361
Received: 4 August 2020 / Revised: 13 September 2020 / Accepted: 15 September 2020 / Published: 15 October 2020
(This article belongs to the Special Issue Biopolymers for Medicinal, Macromolecules, and Food Applications)
Natural product extraction is ingenuity that permits the mass manufacturing of specific products in a cost-effective manner. With the aim of obtaining an alternative chitosan supply, the carapace of dead horseshoe crabs seemed feasible. This sparked an investigation of the structural changes and antioxidant capacity of horseshoe crab chitosan (HCH) by γ-irradiation using 60Co source. Chitosan was extracted from the horseshoe crab (Tachypleus gigas; Müller) carapace using heterogeneous chemical N-deacetylation of chitin, followed by the irradiation of HCH using 60Co at a dose-dependent rate of 10 kGy/hour. The average molecular weight was determined by the viscosimetric method. Regarding the chemical properties, the crystal-like structures obtained from γ-irradiated chitosan powders were determined using Fourier transfer infrared (FTIR) spectroscopy and X-ray diffraction (XRD) analyses. The change in chitosan structure was evident with dose-dependent rates between 10 and 20 kGy/hour. The antioxidant properties of horseshoe crab-derived chitosan were evaluated in vitro. The 20 kGy γ-irradiation applied to chitosan changed the structure and reduced the molecular weight, providing sufficient degradation for an increase in antioxidant activity. Our findings indicate that horseshoe crab chitosan can be employed for both scald-wound healing and long-term food preservation due to its buffer-like and radical ion scavenging ability. View Full-Text
Keywords: biopolymer; antioxidant; preservative; chitin; scavenging; natural product biopolymer; antioxidant; preservative; chitin; scavenging; natural product
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MDPI and ACS Style

Pati, S.; Chatterji, A.; Dash, B.P.; Raveen Nelson, B.; Sarkar, T.; Shahimi, S.; Atan Edinur, H.; Binti Abd Manan, T.S.; Jena, P.; Mohanta, Y.K.; Acharya, D. Structural Characterization and Antioxidant Potential of Chitosan by γ-Irradiation from the Carapace of Horseshoe Crab. Polymers 2020, 12, 2361. https://doi.org/10.3390/polym12102361

AMA Style

Pati S, Chatterji A, Dash BP, Raveen Nelson B, Sarkar T, Shahimi S, Atan Edinur H, Binti Abd Manan TS, Jena P, Mohanta YK, Acharya D. Structural Characterization and Antioxidant Potential of Chitosan by γ-Irradiation from the Carapace of Horseshoe Crab. Polymers. 2020; 12(10):2361. https://doi.org/10.3390/polym12102361

Chicago/Turabian Style

Pati, Siddhartha, Anil Chatterji, Bisnu P. Dash, Bryan Raveen Nelson, Tanmay Sarkar, Salwa Shahimi, Hisham Atan Edinur, Teh S. Binti Abd Manan, Paramananda Jena, Yugal K. Mohanta, and Diptikanta Acharya. 2020. "Structural Characterization and Antioxidant Potential of Chitosan by γ-Irradiation from the Carapace of Horseshoe Crab" Polymers 12, no. 10: 2361. https://doi.org/10.3390/polym12102361

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