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Catalysts 2018, 8(5), 201; https://doi.org/10.3390/catal8050201

Immobilization of the β-fructofuranosidase from Xanthophyllomyces dendrorhous by Entrapment in Polyvinyl Alcohol and Its Application to Neo-Fructooligosaccharides Production

1
Instituto de Catálisis y Petroleoquímica, CSIC, 28049 Madrid, Spain
2
Centro de Biología Molecular Severo Ochoa, CSIC-UAM, 28049 Madrid, Spain
3
Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade Lisboa, 1649-003 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Received: 21 April 2018 / Revised: 7 May 2018 / Accepted: 9 May 2018 / Published: 11 May 2018
(This article belongs to the Special Issue Immobilized Biocatalysts)
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Abstract

The β-fructofuranosidase (Xd-INV) from the basidiomycota yeast Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma) is unique in its ability to synthesize neo- fructooligosaccharides (neo-FOS). In order to facilitate its industrial application, the recombinant enzyme expressed in Pichia pastoris (pXd-INV) was immobilized by entrapment in polyvinyl alcohol (PVA) hydrogels. The encapsulation efficiency exceeded 80%. The PVA lenticular particles of immobilized pXd-INV were stable up to approximately 40 °C. Using 600 g/L sucrose, the immobilized biocatalyst synthesized 18.9% (w/w) FOS (59.1 g/L of neokestose, 30.2 g/L of 1-kestose, 11.6 g/L of neonystose and 12.6 g/L of blastose). The operational stability of PVA-immobilized biocatalyst was assayed in a batch reactor at 30 °C. The enzyme preserved its initial activity during at least 7 cycles of 26 h. View Full-Text
Keywords: glycosidases; fructooligosaccharides; prebiotics; enzyme entrapment; immobilization; bioreactors; hydrogels; neokestose glycosidases; fructooligosaccharides; prebiotics; enzyme entrapment; immobilization; bioreactors; hydrogels; neokestose
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Míguez, N.; Gimeno-Pérez, M.; Fernández-Polo, D.; Cervantes, F.V.; Ballesteros, A.O.; Fernández-Lobato, M.; Ribeiro, M.H.; Plou, F.J. Immobilization of the β-fructofuranosidase from Xanthophyllomyces dendrorhous by Entrapment in Polyvinyl Alcohol and Its Application to Neo-Fructooligosaccharides Production. Catalysts 2018, 8, 201.

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